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Questions and Answers
Which method is a colorimetric method for detecting all sugars?
Which method is a colorimetric method for detecting all sugars?
What is the wavelength at which the concentrations are measured by a spectrophotometer in the Anthrone method?
What is the wavelength at which the concentrations are measured by a spectrophotometer in the Anthrone method?
Which solution is prepared by dissolving 173 gm of Rochelle salt and 50 gm of sodium hydroxide in water?
Which solution is prepared by dissolving 173 gm of Rochelle salt and 50 gm of sodium hydroxide in water?
What is the basis of the SCORLE method for estimating reducing sugars?
What is the basis of the SCORLE method for estimating reducing sugars?
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What is the purpose of adding potassium iodide to an acidic medium in the SCORLE method?
What is the purpose of adding potassium iodide to an acidic medium in the SCORLE method?
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Study Notes
Methods for Detecting Sugars
- The Anthrone method is a colorimetric method for detecting all sugars.
Anthrone Method
- The concentrations are measured by a spectrophotometer at a wavelength of 630 nm.
Reagent Preparation
- The Fehling's solution is prepared by dissolving 173 gm of Rochelle salt and 50 gm of sodium hydroxide in water.
SCORLE Method
- The SCORLE method is based on the oxidation of reducing sugars by potassium ferricyanide, resulting in the formation of potassium ferrocyanide.
- The purpose of adding potassium iodide to an acidic medium in the SCORLE method is to oxidize the potassium ferrocyanide back to potassium ferricyanide, releasing iodine in the process.
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Description
Test your knowledge of the quantitation of carbohydrates using the Anthrone method with this quiz. Explore the steps to convert it into a quantitative method and understand the experimental conditions involved in the process.
