Questions and Answers
Which method is a colorimetric method for detecting all sugars?
Anthrone method
What is the wavelength at which the concentrations are measured by a spectrophotometer in the Anthrone method?
620nm
Which solution is prepared by dissolving 173 gm of Rochelle salt and 50 gm of sodium hydroxide in water?
Fehling’s solution (B)
What is the basis of the SCORLE method for estimating reducing sugars?
Signup and view all the answers
What is the purpose of adding potassium iodide to an acidic medium in the SCORLE method?
Signup and view all the answers
Study Notes
Methods for Detecting Sugars
- The Anthrone method is a colorimetric method for detecting all sugars.
Anthrone Method
- The concentrations are measured by a spectrophotometer at a wavelength of 630 nm.
Reagent Preparation
- The Fehling's solution is prepared by dissolving 173 gm of Rochelle salt and 50 gm of sodium hydroxide in water.
SCORLE Method
- The SCORLE method is based on the oxidation of reducing sugars by potassium ferricyanide, resulting in the formation of potassium ferrocyanide.
- The purpose of adding potassium iodide to an acidic medium in the SCORLE method is to oxidize the potassium ferrocyanide back to potassium ferricyanide, releasing iodine in the process.
Studying That Suits You
Use AI to generate personalized quizzes and flashcards to suit your learning preferences.
