Quantitation of Nucleic Acids

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Questions and Answers

Which of the following is one of the main approaches used to quantitate nucleic acids in a solution?

  • Gas chromatography
  • Spectrophotometric analysis (correct)
  • Electrophoresis
  • Mass spectrometry

What is the purpose of quantitating nucleic acids in molecular biology?

  • To identify the specific sequence of nucleic acids
  • To establish the purity of nucleic acids
  • To determine the average concentrations of proteins
  • To optimize the performance of nucleic acid reactions (correct)

What is the function of a spectrophotometer in spectrophotometric analysis?

  • To determine the average concentrations of nucleic acids
  • To tag nucleic acids with a DNA dye
  • To measure the absorbance of ultraviolet light (correct)
  • To establish the purity of nucleic acids

Which type of light do nucleic acids absorb in spectrophotometric analysis?

<p>Ultraviolet light (C)</p> Signup and view all the answers

What is the purpose of UV fluorescence tagging in the quantitation of nucleic acids?

<p>To enhance the visibility of nucleic acids under UV light (D)</p> Signup and view all the answers

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