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Questions and Answers
What is Western blotting used for?
What is Western blotting used for?
- Detecting the presence of DNA in a sample
- Detecting specific proteins in a sample (correct)
- Detecting the presence of carbohydrates in a sample
- Detecting the presence of RNA in a sample
Which of the following is not a method of detection used in Western blotting?
Which of the following is not a method of detection used in Western blotting?
- Radioactive
- Magnetic (correct)
- Colorimetric
- Fluorescent
Which diseases can Western blotting be used to detect proteins in?
Which diseases can Western blotting be used to detect proteins in?
- Arthritis, osteoporosis, and cancer
- Influenza, chickenpox, and measles
- Diabetes, hypertension, and asthma
- HIV, Lyme disease, and hepatitis B infection (correct)
What are probes used for in detection techniques?
What are probes used for in detection techniques?
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What are TaqMan probes and molecular beacons examples of?
What are TaqMan probes and molecular beacons examples of?
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How do TaqMan probes emit fluorescence?
How do TaqMan probes emit fluorescence?
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Which detection method is considered one of the best for quantification but is less sensitive than chemiluminescence?
Which detection method is considered one of the best for quantification but is less sensitive than chemiluminescence?
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Study Notes
- Western blotting is a technique used to detect specific proteins in a sample.
- There are different methods of detection, including colorimetric, radioactive, and fluorescent.
- Western blotting is used for detecting proteins in various diseases, including HIV, Lyme disease, and hepatitis B infection.
- Probes are single-stranded DNA or RNA sequences used to detect complementary sequences in a sample.
- DNA probes are used to detect the presence of DNA or a gene in a sample.
- TaqMan probes and molecular beacons are examples of DNA probes used for gene quantification and expression studies.
- TaqMan probes use the exonuclease activity of Taq DNA polymerase to emit fluorescence.
- Molecular beacons contain a fluorophore and quencher molecule to increase specificity in quantification assays.
- Western blotting is the most sensitive and specific test for determining the size and amount of protein present in any material.
- Fluorescence is considered one of the best methods for quantification, but it is less sensitive than chemiluminescence.
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