Protein and DNA Structure Quiz
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Questions and Answers

Which bond ensures the primary structure of proteins:

  • Glycosidic bond
  • Hydrophobic bond
  • Hydrogen bond
  • Polypeptide bond (correct)
  • Phosphodiester bond

Which bond ensures the secondary structure of protein:

  • Hydrophobic bond
  • Glycosidic bond
  • Polypeptide bond
  • Hydrogen bond (correct)
  • Phosphodiester bond

Which bonds participate in formation of the quaternary structure of proteins:

  • All mentioned above (correct)
  • Hydrophobic bond
  • Hydrogen bond
  • Van der Waals attraction
  • Ionic bond

What is the name of the primary structure of protein?

<p>Polypeptide chain of amino acids (B)</p> Signup and view all the answers

What is the name of the secondary structure of protein?

<p>Alpha helix, Beta sheets (A)</p> Signup and view all the answers

What is an example of quaternary structure of proteins?

<p>Hemoglobin (C)</p> Signup and view all the answers

What kind of bond is responsible for primary structure of DNA?

<p>Phosphodiester bond (B)</p> Signup and view all the answers

Which bonds are responsible for secondary structure of DNA?

<p>Hydrogen bond (B)</p> Signup and view all the answers

Flashcards

Primary Structure of Proteins

The linear sequence of amino acids in a protein chain, determined by the polypeptide bonds between them.

Secondary Structure of Proteins

The local folding patterns of the polypeptide chain, formed by hydrogen bonding between amino acids. Common structures include alpha helixes and beta sheets.

Quaternary Structure of Proteins

The arrangement of multiple polypeptide chains (subunits) in a protein complex, stabilized by various noncovalent interactions like hydrogen bonds, ionic bonds, hydrophobic interactions, and van der Waals forces.

Polypeptide Chain

The primary structure of a protein, consisting of a linear chain of amino acids linked by peptide bonds.

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Alpha Helix & Beta Sheets

Common secondary structures of proteins formed by hydrogen bonding between amino acids. Alpha helixes are spiral-shaped, while beta sheets are flat, sheet-like structures.

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Hemoglobin

A protein composed of four polypeptide subunits that transports oxygen in the blood, demonstrating quaternary structure.

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Primary Structure of DNA

The linear sequence of nucleotides in a DNA molecule, determined by the phosphodiester bonds between them.

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Secondary Structure of DNA

The double helix structure of DNA, formed by hydrogen bonding between complementary bases on two antiparallel strands.

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Polynucleotide Chain

The primary structure of DNA, consisting of a linear chain of nucleotides linked by phosphodiester bonds.

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DNA Double Helix

The secondary structure of DNA, consisting of two antiparallel strands of nucleotides held together by hydrogen bonds.

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Chromosome

A highly organized structure of DNA, protein, and RNA, found in the nucleus of eukaryotic cells, responsible for carrying genetic information.

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Ribosome

A complex cellular structure composed of ribosomal RNA (rRNA) and proteins, responsible for protein synthesis.

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DNA Packaging Levels

The progressive compaction of DNA from a linear chain to a highly condensed chromosome, involving multiple levels of organization with different mechanisms.

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Nucleosome

The basic unit of DNA packaging, consisting of a DNA segment wrapped around a histone octamer.

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Solenoid

A higher level of DNA packing where nucleosomes are further condensed into a helical structure.

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MAR (Matrix Attachment Region)

Specific DNA sequences that attach to the nuclear matrix, contributing to higher-order DNA organization.

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Thymine

One of the four nitrogenous bases found in DNA, forming two hydrogen bonds with adenine.

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Guanine & Cytosine

Two nitrogenous bases found in DNA, forming three hydrogen bonds between them.

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Uracil

One of the four nitrogenous bases found in RNA, replacing thymine in DNA.

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Phosphodiester Bond

The covalent bond that links nucleotides together in a polynucleotide chain.

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Hydrogen Bond

A weak noncovalent interaction between a hydrogen atom and an electronegative atom, like oxygen or nitrogen, important for the structure of DNA and proteins.

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rER (Rough Endoplasmic Reticulum)

A network of interconnected membranes studded with ribosomes, involved in protein synthesis and modification.

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sER (Smooth Endoplasmic Reticulum)

A network of interconnected membranes lacking ribosomes, involved in lipid synthesis, detoxification, and other metabolic processes.

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GA (Golgi Apparatus)

A stack of flattened membrane-bound sacs involved in further processing, sorting, and packaging of proteins and lipids.

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Lysosome

A membrane-bound organelle containing hydrolytic enzymes, responsible for intracellular digestion and recycling.

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Acid Lipase

An enzyme found in lysosomes that breaks down lipids.

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Insulin Receptor

A protein found on the cell surface that binds insulin, triggering cellular responses to glucose regulation.

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Tubulin

A protein that assembles into microtubules, involved in cell shape, movement, and intracellular transport.

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Mitochondria

A double-membrane-bound organelle responsible for ATP production through cellular respiration.

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Cardiolipin

A special lipid found in the inner membrane of mitochondria, important for mitochondrial function.

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Active Transport

The movement of molecules across a cell membrane against their concentration gradient, requiring energy input.

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Simple Diffusion

The passive movement of molecules across a cell membrane from a region of higher concentration to a region of lower concentration, driven by the concentration gradient.

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Na-K Pump

A transmembrane protein involved in active transport, pumping sodium ions out of the cell and potassium ions into the cell, maintaining the electrochemical gradient.

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Study Notes

Protein Structure

  • Primary Structure: Polypeptide chain of amino acids linked by peptide bonds.
  • Secondary Structure: Alpha helix or Beta sheets, stabilized by hydrogen bonds between amino acid backbones.
  • Tertiary Structure: Complex 3D folding of the polypeptide chain, stabilized by various interactions (hydrogen bonds, hydrophobic interactions, disulfide bridges, ionic bonds).
  • Quaternary Structure: Interaction of multiple polypeptide chains to form a functional protein complex, held together by various bonds (hydrogen bonds, ionic bonds, hydrophobic interactions).

DNA Structure

  • Primary Structure:  Polynucleotide chain composed of nucleotides linked by phosphodiester bonds.
  • Secondary Structure: Double helix formed by two polynucleotide strands held together by hydrogen bonds between complementary base pairs (adenine with thymine, guanine with cytosine).

Cellular Processes

  • Ribosome Function: Synthesizing proteins, either free or bound in the endoplasmic reticulum.
  • Golgi Apparatus (GA) Function: Modifying, sorting, and packaging proteins.
  • Endoplasmic Reticulum (ER) Function: Modifying and transporting proteins made by ribosomes.
  • Lipid Synthesis: Endoplasmic reticulum, for lipids and proteins
  • Cellular Transport: Facilitated diffusion, active transport as mechanism across cell membranes.
  • Detoxification: Endoplasmic Reticulum for detoxification.
  • Nucleosome Level: DNA is packed around histone proteins.
  • Solenoid Level: Nucleosomes are organized into a tightly wound solenoid structure.
  • Third Level (Loop): DNA interacts with scaffolding proteins.

Molecular Biology Test - Specific Questions

  • DNA monomer: dNTP
  • Acidic pH (pH=5): Lysosome

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Description

Test your knowledge on the structures of proteins and DNA, including their primary, secondary, tertiary, and quaternary arrangements. Explore the complexities of ribosome function and cellular processes related to these essential biomolecules.

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