Podcast
Questions and Answers
What is the purpose of the polymerase chain reaction (PCR)?
What is the purpose of the polymerase chain reaction (PCR)?
- To rapidly analyze ancient samples of DNA
- To amplify the size of DNA
- To identify infectious agents
- To rapidly make millions to billions of copies of a specific DNA sample (correct)
At what temperature is the denaturation of dsDNA template performed in PCR?
At what temperature is the denaturation of dsDNA template performed in PCR?
- 80–85°C
- 50–70°C
- 92–95°C (correct)
- 72°C
What is the function of Deoxynucleoside triphosphates (dNTPs) in PCR?
What is the function of Deoxynucleoside triphosphates (dNTPs) in PCR?
- To act as primers for DNA replication
- To serve as energy sources for DNA synthesis (correct)
- To regulate the pH of the buffer
- To stabilize the DNA polymerase
Which chemical component of PCR is responsible for reducing the energy barrier during DNA synthesis?
Which chemical component of PCR is responsible for reducing the energy barrier during DNA synthesis?
What is the main function of thermostable DNA polymerase in PCR?
What is the main function of thermostable DNA polymerase in PCR?
What is the purpose of PCR primers in the polymerase chain reaction (PCR)?
What is the purpose of PCR primers in the polymerase chain reaction (PCR)?
What is the purpose of the denaturation step in PCR?
What is the purpose of the denaturation step in PCR?
What is the function of Multiplex PCR?
What is the function of Multiplex PCR?
What is the purpose of the extension/elongation step in PCR?
What is the purpose of the extension/elongation step in PCR?
What is the significance of the initialization step in PCR?
What is the significance of the initialization step in PCR?
PCR is a method used to make millions to billions of copies of a specific RNA sample.
PCR is a method used to make millions to billions of copies of a specific RNA sample.
PCR is not commonly used in medical laboratory research for applications in biomedical research and criminal forensics.
PCR is not commonly used in medical laboratory research for applications in biomedical research and criminal forensics.
The denaturation of dsDNA template in PCR is performed at approximately 100°C.
The denaturation of dsDNA template in PCR is performed at approximately 100°C.
PCR does not involve amplifying copies of very small amounts of DNA sequences in a series of cycles of temperature changes.
PCR does not involve amplifying copies of very small amounts of DNA sequences in a series of cycles of temperature changes.
PCR primers are not one of the chemical components of PCR.
PCR primers are not one of the chemical components of PCR.
During the denaturation step in PCR, hydrogen bonds between complementary bases are strengthened, leading to the formation of double-stranded DNA molecules.
During the denaturation step in PCR, hydrogen bonds between complementary bases are strengthened, leading to the formation of double-stranded DNA molecules.
Hot-start PCR is initiated at a temperature of 94–96 °C to activate extremely thermostable polymerases.
Hot-start PCR is initiated at a temperature of 94–96 °C to activate extremely thermostable polymerases.
Multiplex PCR involves amplifying only a single DNA sequence at a time to produce amplicons of varying sizes.
Multiplex PCR involves amplifying only a single DNA sequence at a time to produce amplicons of varying sizes.
The annealing step in PCR involves lowering the reaction temperature to 70–75 °C for 30–40 seconds.
The annealing step in PCR involves lowering the reaction temperature to 70–75 °C for 30–40 seconds.
Touchdown PCR is a type of PCR that initially has a high annealing temperature which is gradually decreased in subsequent cycles.
Touchdown PCR is a type of PCR that initially has a high annealing temperature which is gradually decreased in subsequent cycles.
