Nucleic Acid Extraction Techniques

<p>Polymerase Chain Reaction (A)</p> Signup and view all the answers

<p>Pre-PCR steps (A)</p> Signup and view all the answers

<p>Only white blood cells (C)</p> Signup and view all the answers

<p>White blood cells (C)</p> Signup and view all the answers

<p>They lack nuclei. (B)</p> Signup and view all the answers

<p>It includes white blood cells that contain DNA. (A)</p> Signup and view all the answers

<p>They lack nuclei. (B)</p> Signup and view all the answers

<p>As a medium for isolating white blood cells (C)</p> Signup and view all the answers

<p>Environmental studies (B)</p> Signup and view all the answers

<p>It creates a density gradient for cell isolation (A)</p> Signup and view all the answers

<p>They can be used in various scientific applications (A)</p> Signup and view all the answers

<p>White Blood Cell (C)</p> Signup and view all the answers

<p>To keep DNA stable and minimize degradation (A)</p> Signup and view all the answers

<p>It maintains a stable pH and protects DNA (A)</p> Signup and view all the answers

<p>To ensure the integrity and reliability of the DNA (A)</p> Signup and view all the answers

<p>Tris acts as a buffering agent (D)</p> Signup and view all the answers

<p>During DNA extraction processes (A)</p> Signup and view all the answers

<p>It is much more expensive than previous methods. (D)</p> Signup and view all the answers

<p>Intensive Care Unit (A)</p> Signup and view all the answers

<p>5 minutes (C)</p> Signup and view all the answers

<p>It has better quality results. (A)</p> Signup and view all the answers

<p>It provides high-level medical care for critical patients. (C)</p> Signup and view all the answers

<p>To break down the cell wall and membranes (D)</p> Signup and view all the answers

<p>Precipitation of proteins from the DNA sample (B)</p> Signup and view all the answers

<p>Detergents and enzymes that disrupt cellular components (D)</p> Signup and view all the answers

<p>To break down lipids and proteins in cell membranes (C)</p> Signup and view all the answers

<p>To ensure DNA is free from contaminants (A)</p> Signup and view all the answers

The most advanced and expensive method of manufacturing, taking only 5 minutes to complete.

A specialized medical unit that provides intensive care for critically ill patients.

The amount of DNA present in a given volume of solution.

The process of separating DNA fragments based on their size and charge.

The steps taken to analyze and evaluate DNA before the PCR process.

A laboratory technique used to amplify specific DNA sequences.

The range of DNA concentration typically used in PCR reactions, from 10 nanograms to 10 micrograms per microliter.

A substance used to create a density gradient in a centrifuge, allowing separation of different cell types based on their density.

A sample of blood collected for analysis, containing white blood cells, red blood cells, plasma, and other components.

White blood cells are a type of blood cell that play a crucial role in the immune system, defending the body against infections and diseases.

A technique that uses centrifugal force to separate substances based on their density. Heavier components sediment to the bottom, while lighter components remain at the top.

Genetic material found in the nucleus of cells, containing instructions for building and maintaining an organism.

A type of biological sample commonly used in DNA analysis. It contains both white blood cells (WBCs) and red blood cells (RBCs).

Red blood cells, also known as erythrocytes, are responsible for carrying oxygen throughout the body.

A type of blood sample that contains both white blood cells (WBCs) and red blood cells (RBCs).

While both white blood cells (WBCs) and red blood cells (RBCs) are found in blood, only WBCs contain DNA because RBCs lack nuclei.

A solution used in DNA extraction to break down cell walls and membranes, releasing DNA.

A step in DNA extraction that removes proteins from the sample, leaving behind pure DNA.

A key component of lysis buffer, these chemicals break down cell structures, allowing DNA to be released.

Enzymes present in lysis buffer that help degrade specific cell components, allowing for the release of DNA.

The process of separating and isolating DNA from a sample, often involving steps like lysis, protein precipitation, and purification.

Tris-EDTA is a type of buffer solution, which helps maintain a stable pH (acidity level). It also helps to keep DNA stable and protects it from breaking down.

Tris-EDTA contains two main components: Tris (tris(hydroxymethyl)aminomethane) and EDTA (ethylenediaminetetraacetic acid). Tris is a base that helps maintain pH, while EDTA binds to metal ions that could damage DNA.

Tris-EDTA helps preserve DNA during extraction by preventing degradation from enzymes like DNases. It also helps to protect DNA from contamination by other substances that might affect its integrity.

Tris-EDTA's pH buffering ability is important for maintaining an optimal environment for DNA. This ensures that the structure and function of DNA remain stable.

Tris-EDTA is frequently used in molecular biology research, specifically for procedures involving DNA. This includes gene cloning, PCR (polymerase chain reaction), and sequencing.