10 Questions
What is the role of Taq polymerase in the PCR process?
Extending DNA strands during PCR
How many copies of the target DNA are produced after 20 PCR cycles?
$2^{10}$
Which step of PCR involves the separation of DNA strands?
Denaturation
What is the purpose of annealing in the PCR process?
Binding primers to the template DNA
Why is it important to know the size of the PCR product?
To confirm the correct amplification of the target sequence
In which step of PCR does Taq DNA polymerase add nucleotides to extend DNA strands?
Extension
What does PCR stand for?
Polymerase Chain Reaction
How does Taq polymerase differ from other polymerases?
It is heat-stable
What could happen if the denaturation step is skipped in a PCR cycle?
The DNA strands will not separate
What happens if there are errors in primer design for a PCR reaction?
The target sequence won't be amplified correctly
Test your knowledge on nucleic acid extraction and amplification processes, including isolation, purification, and amplification of specific target sequences through PCR. Explore different methods used for target detection in the final stage.
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