Plant Tissue Culture: Micronutrients and Nutritional Requirements
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Questions and Answers

What is the ideal pH range for adjusting the culture media during the preparation of ½ MS medium?

  • 5.4 or 5.5
  • 5.8 or 5.9
  • 6.0 or 6.1
  • 5.6 or 5.7 (correct)

    • What is the primary objective of Stage 0 in the process of seed explant selection?

  • To induce root formation using auxins
  • To select the appropriate seed explant for further processing (correct)
  • To establish mother stock culture
  • To multiply shoots using cytokinins

    • What is the recommended method to collect seeds for their culture?

  • Gather fruit when it is dried
  • Gather fruit when it is ripe (correct)
  • Gather fruit when it is rotten
  • Gather fruit when it is unripe

    • What is the primary purpose of using cytokinins in Stage II?

    <p>To multiply shoots</p> Signup and view all the answers

    What is the recommended tool to use when dividing small seeds into groups?

    <p>Magnifying lens</p> Signup and view all the answers

    What is the purpose of taking a photo of the collected seeds?

    <p>To record the size of the seeds</p> Signup and view all the answers

    What is the quantity of MS medium required to prepare 1 L of ½ MS medium?

    <p>2.2 g</p> Signup and view all the answers

    What is the primary function of auxins in Stage III?

    <p>To induce root formation</p> Signup and view all the answers

    What is the recommended way to divide the collected seeds?

    <p>Into 5 groups</p> Signup and view all the answers

    What is the purpose of Stage IV in the process of seed explant selection?

    <p>To acclimatize or harden the plantlets</p> Signup and view all the answers

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