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COPY: Plant Cell Culture and Applications: Introduction

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COPY: Plant Cell Culture and Applications: Introduction

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Questions and Answers

What is the basic technique of plant tissue culture?

Growing the cultures in the growth chamber or plant tissue culture room

Transfer of plants to the transgenic green-house or field conditions

Selection of suitable explants and surface sterilization of the explants (correct)

Regeneration of shoots from cultured plant tissues and their elongation

What is included in the typical plant tissue culture nutrient medium?

Carbonated beverages, vitamins, proteins, and growth regulators

Inorganic salts, sugars, fats, and growth hormones

Inorganic salts, vitamins, amino acids, and growth regulators (correct)

Inorganic substances, vitamins, nucleic acids, and growth promoters

What physical conditions are required for growing cultures in plant tissue culture?

Natural light (8 h photoperiod), temperature (~18oC), and relative humidity (80-90%)

Artificial light (16 h photoperiod), temperature (~26oC), and relative humidity (50-60%) (correct)

Artificial light (24 h photoperiod), temperature (~30oC), and relative humidity (40-50%)

Natural light (12 h photoperiod), temperature (~22oC), and relative humidity (60-70%)

What is responsible for the 'second green revolution' in plant cell culture techniques?

Introduction of foreign genes into crop plants using cell and tissue culture systems (A) Signup and view all the answers

What are the suitable explants for plant tissue culture?

Shoot tip, leaf, cotyledon, and hypocotyls (B) Signup and view all the answers

What is totipotency in the context of plant cell culture?

The ability of a plant cell to regenerate into a whole plant on a defined artificial nutrient medium under suitable conditions (C) Signup and view all the answers

Who is regarded as the 'Father of Plant Tissue Culture'?

Gottlieb Haberlandt (A) Signup and view all the answers

What did Gottlieb Haberlandt predict in the field of plant tissue culture?

All of the above (D) Signup and view all the answers

During which period were attempts made to culture isolated plant organs such as roots and shoot apices?

1902 - 1930s (A) Signup and view all the answers

What unique property of a plant cell is the plant cell culture based on?

Totipotency (D) Signup and view all the answers