Plant Cell Culture and Totipotency
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Questions and Answers

What is totipotency in the context of plant cell culture?

  • The ability of a plant cell to regenerate into a whole plant on a defined artificial nutrient medium (correct)
  • The capacity of a single cell to both grow and divide
  • The ability of a plant cell to grow and divide in a self regulatory fashion
  • The ability of a plant cell to resist artificial nutrient medium

Who first attempted to cultivate mechanically isolated plant leaf cells on a simple nutrient medium?

  • Schwann
  • Later researchers
  • Gottlieb Haberlandt (correct)
  • Schleiden

What concept was proposed by Gottlieb Haberlandt in plant tissue culture?

  • The concept of growth hormones
  • The use of embryo sac fluids
  • The cultivation of artificial embryos from somatic cells
  • All of the above (correct)

During which period were attempts made to culture the isolated plant organs such as roots and shoot apices?

<p>1902 - 1930s (C)</p> Signup and view all the answers

Who first drew attention to the fact that a single cell has the capacity to both grow and divide in a self regulatory fashion?

<p>Schwann (D)</p> Signup and view all the answers

What was stated in the 'cell theory' proposed by Schleiden and Schwann?

<p>Totipotency of plant cells (B)</p> Signup and view all the answers

What is the basic technique of plant tissue culture?

<p>Inoculating the explants onto suitable nutrient medium under sterile conditions (B)</p> Signup and view all the answers

What is a key component of the plant tissue culture nutrient medium?

<p>Sucrose (B)</p> Signup and view all the answers

What conditions are typically provided in the growth chamber or plant tissue culture room?

<p>Artificial light, appropriate temperature, and relative humidity (B)</p> Signup and view all the answers

Which era involved the introduction of foreign genes into crop plants using cell and tissue culture systems?

<p>Second green revolution (C)</p> Signup and view all the answers

What is responsible for the regeneration of complete plants from cultured tissues and cells?

<p>In vitro morphogenesis (B)</p> Signup and view all the answers

What are the suitable explants used in plant tissue culture?

<p>Shoot tip, leaf, cotyledon, and hypocotyls (A)</p> Signup and view all the answers

What is used for surface sterilization of the explants in plant tissue culture?

<p>Disinfectants like bleach (A)</p> Signup and view all the answers

What is the purpose of transferring plants to the transgenic greenhouse or field conditions?

<p>To acclimatize the plants to outdoor conditions (C)</p> Signup and view all the answers

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