Plant Cell Culture and Totipotency
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Questions and Answers

What is totipotency in the context of plant cell culture?

  • The ability of a plant cell to regenerate into a whole plant on a defined artificial nutrient medium (correct)
  • The capacity of a single cell to both grow and divide
  • The ability of a plant cell to grow and divide in a self regulatory fashion
  • The ability of a plant cell to resist artificial nutrient medium
  • Who first attempted to cultivate mechanically isolated plant leaf cells on a simple nutrient medium?

  • Schwann
  • Later researchers
  • Gottlieb Haberlandt (correct)
  • Schleiden
  • What concept was proposed by Gottlieb Haberlandt in plant tissue culture?

  • The concept of growth hormones
  • The use of embryo sac fluids
  • The cultivation of artificial embryos from somatic cells
  • All of the above (correct)
  • During which period were attempts made to culture the isolated plant organs such as roots and shoot apices?

    <p>1902 - 1930s</p> Signup and view all the answers

    Who first drew attention to the fact that a single cell has the capacity to both grow and divide in a self regulatory fashion?

    <p>Schwann</p> Signup and view all the answers

    What was stated in the 'cell theory' proposed by Schleiden and Schwann?

    <p>Totipotency of plant cells</p> Signup and view all the answers

    What is the basic technique of plant tissue culture?

    <p>Inoculating the explants onto suitable nutrient medium under sterile conditions</p> Signup and view all the answers

    What is a key component of the plant tissue culture nutrient medium?

    <p>Sucrose</p> Signup and view all the answers

    What conditions are typically provided in the growth chamber or plant tissue culture room?

    <p>Artificial light, appropriate temperature, and relative humidity</p> Signup and view all the answers

    Which era involved the introduction of foreign genes into crop plants using cell and tissue culture systems?

    <p>Second green revolution</p> Signup and view all the answers

    What is responsible for the regeneration of complete plants from cultured tissues and cells?

    <p>In vitro morphogenesis</p> Signup and view all the answers

    What are the suitable explants used in plant tissue culture?

    <p>Shoot tip, leaf, cotyledon, and hypocotyls</p> Signup and view all the answers

    What is used for surface sterilization of the explants in plant tissue culture?

    <p>Disinfectants like bleach</p> Signup and view all the answers

    What is the purpose of transferring plants to the transgenic greenhouse or field conditions?

    <p>To acclimatize the plants to outdoor conditions</p> Signup and view all the answers

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