14 Questions
What is totipotency in the context of plant cell culture?
The ability of a plant cell to regenerate into a whole plant on a defined artificial nutrient medium
Who first attempted to cultivate mechanically isolated plant leaf cells on a simple nutrient medium?
Gottlieb Haberlandt
What concept was proposed by Gottlieb Haberlandt in plant tissue culture?
All of the above
During which period were attempts made to culture the isolated plant organs such as roots and shoot apices?
1902 - 1930s
Who first drew attention to the fact that a single cell has the capacity to both grow and divide in a self regulatory fashion?
Schwann
What was stated in the 'cell theory' proposed by Schleiden and Schwann?
Totipotency of plant cells
What is the basic technique of plant tissue culture?
Inoculating the explants onto suitable nutrient medium under sterile conditions
What is a key component of the plant tissue culture nutrient medium?
Sucrose
What conditions are typically provided in the growth chamber or plant tissue culture room?
Artificial light, appropriate temperature, and relative humidity
Which era involved the introduction of foreign genes into crop plants using cell and tissue culture systems?
Second green revolution
What is responsible for the regeneration of complete plants from cultured tissues and cells?
In vitro morphogenesis
What are the suitable explants used in plant tissue culture?
Shoot tip, leaf, cotyledon, and hypocotyls
What is used for surface sterilization of the explants in plant tissue culture?
Disinfectants like bleach
What is the purpose of transferring plants to the transgenic greenhouse or field conditions?
To acclimatize the plants to outdoor conditions
Test your knowledge of plant cell culture and the concept of totipotency, which refers to the ability of a plant cell to regenerate into a whole plant. Explore the unique properties of plant cells and their applications in laboratory conditions.
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