Podcast
Questions and Answers
What is the ideal diameter of the rootstock for side-stub grafting?
What is the ideal diameter of the rootstock for side-stub grafting?
How many scions should be inserted into a stock of 10 cm diameter?
How many scions should be inserted into a stock of 10 cm diameter?
What is the angle of the oblique cut made into the rootstock for side-stub grafting?
What is the angle of the oblique cut made into the rootstock for side-stub grafting?
What is the principle behind micropropagation?
What is the principle behind micropropagation?
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What is the benefit of micropropagation in plants where vegetative propagation is difficult?
What is the benefit of micropropagation in plants where vegetative propagation is difficult?
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What is the advantage of micropropagation in terms of space and time?
What is the advantage of micropropagation in terms of space and time?
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Which fruit crop was the first to be commercially propagated using micropropagation?
Which fruit crop was the first to be commercially propagated using micropropagation?
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What is the purpose of waxing the cut surfaces after grafting?
What is the purpose of waxing the cut surfaces after grafting?
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When is the best time to perform cleft grafting?
When is the best time to perform cleft grafting?
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What is the length of the scion in side-stub grafting?
What is the length of the scion in side-stub grafting?
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Study Notes
Micropropagation
- Micropropagation is a method of producing plants from small plant parts, tissue, or cells under aseptic conditions, based on the principle of totipotency.
- Strawberry was the first fruit crop to be commercially propagated using micropropagation technique.
Advantages of Micropropagation
- Large-scale multiplication of virus-free planting materials in limited time and space.
- Year-round production possible.
- Beneficial for plants where vegetative propagation is not easy, such as papaya.
- Production of secondary metabolites.
- Helps in long-term storage in lesser space.
Methods of Micropropagation
- Meristem tip culture: eliminates virus from infected plants, consists of 1 or 2 pairs of leaf primordia.
- Callus culture: unorganised mass of parenchymatous cells, undergoes organogenesis in two steps.
- Anther culture: produces homozygous plants, first reported in Datura by Guha and Maheshawari (1959).
- Cell suspension culture: involves homogenizing a piece of callus into liquid medium and shaking until medium becomes cloudy with suspended cells.
- Ovule culture: unfertilized or just fertilized ovule excised and cultured.
Root Cuttings
- Used for plants that produce suckers freely, such as blackberry and raspberry.
- Preparation of root cuttings involves cutting roots into pieces, 1 cm thick and 10-15 cm long, in late winter or early spring.
- Correct polarity is important, with the proximal end having a straight cut and the distal end having a slanting cut.
Budding
- Involves attaching a scion with only one bud to a stock.
- Scion is prepared by trimming the base to a wedge shape, exactly the same size and shape as the V-wedge on the stock.
- Waxing is essential to prevent drying out of the cut surfaces.
Grafting
- Detached scion grafting: also known as side graftage, useful for trees that are too large for whip or tongue grafting but not large enough for cleft or wedge grafting.
- Side-stub grafting: involves making an oblique cut into the rootstock at an angle of about 20-30°, with a scion that is about 7.5 cm long and relatively thin.
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Description
This quiz covers various methods of plant biotechnology, including micropropagation, embryo rescue, and breeding cycle shortening. It also explores the uses of meristem tip culture and callus culture.
