Plant Biotechnology and Tissue Culture
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Plant Biotechnology and Tissue Culture

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Questions and Answers

Which type of explant is typically used for direct or indirect organogenesis?

  • Juvenile shoot tips
  • Mature seeds
  • Young leaves (correct)
  • Fully differentiated roots
  • What is a common reason to avoid using in vivo explants other than seeds, shoot tips, or nodal segments?

  • They can be highly differentiated (correct)
  • They are more prone to disease
  • Their availability is limited
  • They do not support meristem culture
  • Which of the following is classified under organized structure culture?

  • Suspension culture
  • Embryo culture (correct)
  • Protoplast culture
  • Callus culture
  • Which type of culture involves the growth and maintenance of unorganized cell masses?

    <p>Callus culture</p> Signup and view all the answers

    What is the primary content of an isolated root culture?

    <p>Unattached root systems</p> Signup and view all the answers

    Which of the following is NOT considered a type of unorganized structure culture?

    <p>Meristem culture</p> Signup and view all the answers

    Which explants are typically avoided for culture establishment due to physiological stress concerns?

    <p>Fully differentiated tissue</p> Signup and view all the answers

    In which type of culture are fertilized or unfertilized zygotic embryos used?

    <p>Embryo culture</p> Signup and view all the answers

    What principle did Haberlandt propose that is foundational to plant tissue culture?

    <p>Totipotency</p> Signup and view all the answers

    Which hormone was discovered to play a crucial role in plant growth according to the content?

    <p>Indole acetic acid</p> Signup and view all the answers

    Who optimized the growth medium for plants under in vitro conditions?

    <p>Murashige and Skoog</p> Signup and view all the answers

    What do protoplast fusion techniques involve?

    <p>Merging isolated plant protoplasts</p> Signup and view all the answers

    What technique is used to preserve plant cells at ultra-low temperatures?

    <p>Cryopreservation</p> Signup and view all the answers

    Which process involves the production of haploid plants through specific plant culture methods?

    <p>Anther and pollen culture</p> Signup and view all the answers

    Which type of plant materials can be used to create synthetic seeds?

    <p>Embryos</p> Signup and view all the answers

    What is a significant outcome of somaclonal variations in tissue culture?

    <p>Enhanced disease resistance</p> Signup and view all the answers

    Who is credited with discovering that cultured root and stem tips can produce buds?

    <p>Vöchting</p> Signup and view all the answers

    What is the primary purpose of using callus culture in plant tissue culture?

    <p>To generate a mass of undifferentiated cells</p> Signup and view all the answers

    What is the primary goal of anther culture in plant tissue culture?

    <p>To obtain haploid plants through somatic embryo formation</p> Signup and view all the answers

    Which of the following is NOT an advantage of plant tissue culture?

    <p>Production of genetically diverse plants</p> Signup and view all the answers

    What challenge is associated with genetic uniformity in tissue-cultured plants?

    <p>Higher susceptibility to new pests and diseases</p> Signup and view all the answers

    What does somaclonal variation refer to in tissue-cultured plants?

    <p>Genetic variations leading to unexpected traits</p> Signup and view all the answers

    What is a key benefit of using plant tissue culture for rare and endangered species?

    <p>Preservation and propagation of these species</p> Signup and view all the answers

    Which of the following statements about the disadvantages of plant tissue culture is true?

    <p>High technical expertise required can make it expensive.</p> Signup and view all the answers

    How does tissue culture facilitate the production of secondary metabolites?

    <p>By cultivating specific plant cells under controlled conditions</p> Signup and view all the answers

    Why can plant tissue culture be performed year-round?

    <p>It is independent of weather and seasonal changes.</p> Signup and view all the answers

    What is the main focus of plant biotechnology?

    <p>The alteration of living and nonliving materials for agricultural benefits</p> Signup and view all the answers

    What does the term 'totipotency' refer to in plant tissue culture?

    <p>The ability of any plant cell to form a whole plant under proper nourishment</p> Signup and view all the answers

    Which of the following statements about plant tissue culture is true?

    <p>Explants are isolated parts from healthy and intact plants.</p> Signup and view all the answers

    What is the concept of 'plasticity' in the context of plant tissues?

    <p>The ability of an organism to alter its phenotype based on environmental conditions</p> Signup and view all the answers

    What was a major advancement in plant biotechnology compared to traditional methods?

    <p>The application of tissue culture techniques</p> Signup and view all the answers

    Under what conditions are most plant tissue cultures maintained?

    <p>Under aseptic conditions</p> Signup and view all the answers

    Which of the following is an application of plant tissue culture?

    <p>Cultivating genetically identical plants for research</p> Signup and view all the answers

    What can be considered a disadvantage of plant tissue culture?

    <p>It can be expensive and requires technical expertise.</p> Signup and view all the answers

    Study Notes

    Plant Biotechnology

    • Plant biotechnology involves using science and technology to alter plants or their parts for knowledge, goods, and services
    • It encompasses techniques for screening and genetic manipulation of plants
    • Traditional breeding techniques were used in the past: selection, hybridization (crossing)
    • Plant tissue culture techniques are frequently used today

    Plant Tissue Culture

    • Uses isolated parts of plants called explants
    • Explants are cultured on a nutrient medium, which replaces the environment they were originally in
    • Cultures are usually maintained in aseptic conditions. Exceptions include experiments on phytopathology and co-culture studies
    • The concept of plant tissue culture is based on totipotency and plasticity
      • Totipotency: any plant cell can become a whole plant with nourishment
      • Plasticity: the ability of an organism to change its phenotype in response to different environments

    Brief History of Plant Tissue Culture

    • 1756: Henri Louis observed that cells form at wound sites in elm plants
    • 1878: Vöchting observed that the upper part of a shoot produces buds and the lower part produces callus and roots
    • 1902: Gottlieb Haberlandt proposed the principle of totipotency
    • 1922: Kotte and Robbins cultured root and stem tips
    • 1926: Fritz Went discovered the first plant growth hormone, indole acetic acid
    • 1939: Gautheret and Nobecourt worked independently on callus cultures
    • 1946: Ernest Ball produced plants from shoot tip culture
    • 1962: Murashige and Skoog optimized a medium for plant growth in vitro
    • 1964: Guha and Maheshwari produced haploid plants from anther culture
    • 1970: Power et. al. fused isolated plant protoplasts

    Importance of Plant Tissue Culture

    • Rapid Clonal Propagation: Produces many genetically identical plants from a single parent plant. Useful for valuable or rare species
    • Year-Round Propagation: Tissue culture is not affected by seasons, unlike traditional methods
    • Disease-Free Plants: Starts with sterile explants, which are free from diseases, pests, and pathogens
    • Preservation of Rare or Endangered Species: Helps conserve and grow threatened species
    • Genetic Modification: Introduces desired traits such as pest and disease resistance, environmental stress tolerance
    • Production of Secondary Metabolites: Produces pharmaceuticals, essential oils, and other valuable plant compounds
    • Storage and Banking of Genetic Material: Long-term storage of seeds, embryos, and meristems to preserve genetic diversity

    Disadvantages of Plant Tissue Culture

    • Labor-Intensive: Requires technical expertise and labor, making it expensive
    • Genetic Uniformity: Can lead to lack of diversity, making plants susceptible to new pests and diseases
    • Risk of Somaclonal Variation: Genetic variations that can occur in tissue-cultured plants, resulting in unexpected traits or mutations

    Rules for Explant Selection

    • Select superior-quality, disease-free plants and explant types

    • Young leaves (in vitro leaves) are used for direct or indirect organogenesis or somatic embryogenesis

    • Other explants, except seeds, shoot tips, or nodal segments, are avoided, as the tissue is highly differentiated. Forcing it to dedifferentiate and redifferentiate can cause physiological stress.

    Types of Plant Tissue Cultures

    • Organized Structure Culture:
      • Meristem Culture: Excised shoot apices with apical meristematic dome, with or without leaf primordia
      • Shoot Tip Culture: Excised shoot tips or buds larger than shoot apices. Used to establish meristem cultures, typically with several leaf primordia
      • Node Culture: Separate lateral buds with a small piece of stem tissue. Can have single or multiple nodes
      • Isolated Root Culture: Growth of roots unconnected to shoots. Might obtain a branched root system
      • Embryo Culture: Fertilized or unfertilized zygotic (seed) embryos are dissected out of seeds or fruits and cultured until they become seedlings
    • Unorganized Structure Culture
      • Callus Culture: Growth of unorganized cell masses from plant organs, tissues, or previously cultured cells
      • Suspension Cell Culture: Populations of plant cells and small cell clumps dispersed in an agitated, aerated, liquid medium
      • Protoplast Culture: Cultures of plant cells without a cell wall
      • Anther Culture: Cultures of complete anthers containing immature pollen microspores. Aim is to obtain haploid plants through somatic embryo formation

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    Description

    Explore the fascinating world of plant biotechnology, focusing on the techniques and history of plant tissue culture. Learn about the critical concepts of totipotency and plasticity that are fundamental to the manipulation of plant cells. This quiz will challenge your understanding of how science shapes our interaction with plants.

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