Podcast
Questions and Answers
Which type of restriction enzyme is non-ATP-dependent and cuts within the recognition sequence?
Which type of restriction enzyme is non-ATP-dependent and cuts within the recognition sequence?
- Type IV
- Type I
- Type III
- Type II (correct)
What is the purpose of agarose gel electrophoresis in molecular biology?
What is the purpose of agarose gel electrophoresis in molecular biology?
- To visualize different sized DNA fragments (correct)
- To identify restriction enzymes
- To clone DNA fragments
- To cut foreign DNA
What is the purpose of the multiple cloning site in an expression vector?
What is the purpose of the multiple cloning site in an expression vector?
- To replicate the vector in the host cell
- To insert DNA fragments with multiple recognition sites (correct)
- To purify the recombinant protein
- To induce expression of the target gene
Which technique is used to amplify specific DNA sequences in the laboratory?
Which technique is used to amplify specific DNA sequences in the laboratory?
Which field of biotechnology focuses on the manipulation of microorganisms?
Which field of biotechnology focuses on the manipulation of microorganisms?
During which period did the manipulation of genetic material become prominent in biotechnology?
During which period did the manipulation of genetic material become prominent in biotechnology?
What is the main focus of agricultural biotechnology?
What is the main focus of agricultural biotechnology?
Which field of biotechnology involves the analysis of genomes using bioinformatics?
Which field of biotechnology involves the analysis of genomes using bioinformatics?
What is the focus of pharmaceutical sciences in biotechnology?
What is the focus of pharmaceutical sciences in biotechnology?
Which approach is used to screen a greater number of strain variants in Adaptive Laboratory Evolution?
Which approach is used to screen a greater number of strain variants in Adaptive Laboratory Evolution?
What is the purpose of protein engineering?
What is the purpose of protein engineering?
Which technique allows screening of protein-protein binding interactions and enriching mutational variants?
Which technique allows screening of protein-protein binding interactions and enriching mutational variants?
What is the purpose of directed evolution in the context of peptides and proteins?
What is the purpose of directed evolution in the context of peptides and proteins?
What is the purpose of non-natural amino acids in peptide/protein engineering?
What is the purpose of non-natural amino acids in peptide/protein engineering?
Which of the following is a downside of tag-and-modify peptide modification?
Which of the following is a downside of tag-and-modify peptide modification?
What is the primary advantage of click reactions in peptide modification?
What is the primary advantage of click reactions in peptide modification?
What is the purpose of antibody-drug conjugates (ADCs)?
What is the purpose of antibody-drug conjugates (ADCs)?
What is the main difference between biochemistry and chemical biology?
What is the main difference between biochemistry and chemical biology?
What is the future direction of biochemical engineering?
What is the future direction of biochemical engineering?
Which of the following is a characteristic of primary cell culture?
Which of the following is a characteristic of primary cell culture?
Which expression system is best for producing large proteins (>100kD)?
Which expression system is best for producing large proteins (>100kD)?
What is the main advantage of using continuous cell lines for cell culture?
What is the main advantage of using continuous cell lines for cell culture?
What is the purpose of cell culture transfection?
What is the purpose of cell culture transfection?
What is the main difference between traditional breeding and genetic engineering?
What is the main difference between traditional breeding and genetic engineering?
Which method of vaccine production involves making recombinant gene to produce recombinant protein as a vaccine?
Which method of vaccine production involves making recombinant gene to produce recombinant protein as a vaccine?
Which type of vaccines allow transport through cell membranes and protect RNA from being broken down?
Which type of vaccines allow transport through cell membranes and protect RNA from being broken down?
Which organisms are commonly used as eukaryotic model organisms for genome editing?
Which organisms are commonly used as eukaryotic model organisms for genome editing?
What is the function of RNAi or post-translational gene silencing?
What is the function of RNAi or post-translational gene silencing?
What is the main function of functional food?
What is the main function of functional food?
Which field of biotechnology focuses on the manipulation of microorganisms?
Which field of biotechnology focuses on the manipulation of microorganisms?
What is the main focus of agricultural biotechnology?
What is the main focus of agricultural biotechnology?
What is the purpose of directed evolution in the context of peptides and proteins?
What is the purpose of directed evolution in the context of peptides and proteins?
What is the purpose of protein engineering?
What is the purpose of protein engineering?
What is the focus of pharmaceutical sciences in biotechnology?
What is the focus of pharmaceutical sciences in biotechnology?
Which technique is commonly used to isolate the fittest strain in Adaptive Laboratory Evolution?
Which technique is commonly used to isolate the fittest strain in Adaptive Laboratory Evolution?
Which field of biotechnology involves the modification of peptides and proteins for enhancement of existing or endowment of novel features?
Which field of biotechnology involves the modification of peptides and proteins for enhancement of existing or endowment of novel features?
Which of the following is a purpose of sequence optimization in peptide engineering?
Which of the following is a purpose of sequence optimization in peptide engineering?
Which technique allows screening of protein-protein binding interactions and enriching mutational variants?
Which technique allows screening of protein-protein binding interactions and enriching mutational variants?
What is the purpose of introducing non-natural amino acids in peptide/protein engineering?
What is the purpose of introducing non-natural amino acids in peptide/protein engineering?
Which type of enzyme is essential in cutting foreign DNA and cuts unmethylated DNA sequences that are palindromic in character along an approximately 6 bp recognition site?
Which type of enzyme is essential in cutting foreign DNA and cuts unmethylated DNA sequences that are palindromic in character along an approximately 6 bp recognition site?
What is the purpose of agarose gel electrophoresis in molecular biology?
What is the purpose of agarose gel electrophoresis in molecular biology?
What is the purpose of the multiple cloning site in an expression vector?
What is the purpose of the multiple cloning site in an expression vector?
What is the primary advantage of recombinant insulin compared to insulin derived from pancreas glands of pigs?
What is the primary advantage of recombinant insulin compared to insulin derived from pancreas glands of pigs?
What is the purpose of strain optimization in metabolic engineering?
What is the purpose of strain optimization in metabolic engineering?
Which method of vaccine production involves making recombinant gene to produce recombinant protein as a vaccine?
Which method of vaccine production involves making recombinant gene to produce recombinant protein as a vaccine?
Which type of vaccines allow transport through cell membranes and protect RNA from being broken down?
Which type of vaccines allow transport through cell membranes and protect RNA from being broken down?
Which organisms are commonly used as eukaryotic model organisms for genome editing?
Which organisms are commonly used as eukaryotic model organisms for genome editing?
What is the main function of functional food?
What is the main function of functional food?
What is the purpose of agarose gel electrophoresis in molecular biology?
What is the purpose of agarose gel electrophoresis in molecular biology?
Which of the following is a disadvantage of using bacterial expression systems for producing large proteins?
Which of the following is a disadvantage of using bacterial expression systems for producing large proteins?
What is the main advantage of using viral expression systems for protein production?
What is the main advantage of using viral expression systems for protein production?
What is the purpose of click chemistry in peptide modification?
What is the purpose of click chemistry in peptide modification?
What is the main focus of chemical biology?
What is the main focus of chemical biology?
What is the main function of antibody-drug conjugates (ADCs)?
What is the main function of antibody-drug conjugates (ADCs)?
Which type of cell culture has a finite lifespan and closely represents tissue characteristics?
Which type of cell culture has a finite lifespan and closely represents tissue characteristics?
What is the main advantage of using continuous cell lines for cell culture?
What is the main advantage of using continuous cell lines for cell culture?
Which method is commonly used for transfection in cell culture?
Which method is commonly used for transfection in cell culture?
What is the main focus of genetically modified organisms (GMOs)?
What is the main focus of genetically modified organisms (GMOs)?
What is the main difference between traditional breeding and genetic engineering?
What is the main difference between traditional breeding and genetic engineering?
Which of the following is a characteristic of primary cell culture?
Which of the following is a characteristic of primary cell culture?
What is the purpose of cell culture transfection?
What is the purpose of cell culture transfection?
What is the main advantage of using continuous cell lines for cell culture?
What is the main advantage of using continuous cell lines for cell culture?
What is the main difference between traditional breeding and genetic engineering?
What is the main difference between traditional breeding and genetic engineering?
Which organisms are commonly used as eukaryotic model organisms for genome editing?
Which organisms are commonly used as eukaryotic model organisms for genome editing?
