10 Questions
Interference of staining: Fixation may cause hindrance of staining of ______.
enzymes
Formaldehyde inactivates 80% of ______ enzyme.
ribonuclease
It has been noted that osmium tetroxide inhibits ______ and eosin staining.
haematoxylin
Changes of optical density by fixation: The fixation may cause the change of optical density of the ______, and the nuclei may look like condensed and hyperchromatic.
nuclei
The fixative can be classified on the basis of the following criteria: A.Nature of fixation Types of Fixation 1-______ fixation
Immersion
2-______ fixation
Coating
3-______ fixation
Vapour
4-______ fixation
Perfusion
5-______ fixation
Freeze-drying
This is the commonest way of fixation in the laboratories.In this technique the whole specimen is immersed in the liquid fixative such as tissue samples are immersed in 10% neutral buffered formalin or cytology smear in 95% ethyl alcohol.Pathology & Cytology 2- ______ fixation
Coating
Learn about the steps involved in pathology and cytology, starting with rapid freezing of thin tissue sections at -160°C through the process of quenching. Discover the different fluids used in the quenching bath and the removal of ice within tissues during preparation.
Make Your Own Quizzes and Flashcards
Convert your notes into interactive study material.
Get started for free
