Pathology & Cytology Steps: Tissue Freezing and Preparation
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Pathology & Cytology Steps: Tissue Freezing and Preparation

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Questions and Answers

Interference of staining: Fixation may cause hindrance of staining of ______.

enzymes

Formaldehyde inactivates 80% of ______ enzyme.

ribonuclease

It has been noted that osmium tetroxide inhibits ______ and eosin staining.

haematoxylin

Changes of optical density by fixation: The fixation may cause the change of optical density of the ______, and the nuclei may look like condensed and hyperchromatic.

<p>nuclei</p> Signup and view all the answers

The fixative can be classified on the basis of the following criteria: A.Nature of fixation Types of Fixation 1-______ fixation

<p>Immersion</p> Signup and view all the answers

2-______ fixation

<p>Coating</p> Signup and view all the answers

3-______ fixation

<p>Vapour</p> Signup and view all the answers

4-______ fixation

<p>Perfusion</p> Signup and view all the answers

5-______ fixation

<p>Freeze-drying</p> Signup and view all the answers

This is the commonest way of fixation in the laboratories.In this technique the whole specimen is immersed in the liquid fixative such as tissue samples are immersed in 10% neutral buffered formalin or cytology smear in 95% ethyl alcohol.Pathology & Cytology 2- ______ fixation

<p>Coating</p> Signup and view all the answers

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