Oxidation-Fermentation Tests in Microbiology

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18 Questions

Bacteria positive for both MR and VP tests are common.

False

Citrate Utilization Test is used to identify organisms that can use citrate as the sole nitrogen source.

False

Bacteria that can grow in Simmons Citrate Agar medium produce the enzyme citrate-permease.

True

In the Citrate Utilization Test, a color change from green to blue indicates a negative reaction.

False

Christensen's citrate medium uses bromothymol blue as the pH indicator.

False

Bromothymol blue indicator turns from yellow to pink in an acidic pH.

False

In Kligler iron agar (KIA), the medium is identical to TSI except for the presence of glucose as a carbohydrate component.

False

A yellow color indicates an alkaline environment in the oxidation-fermentation (O/F) test using Hugh and Leifson O/F basal medium.

False

The mineral oil overlay in the O/F test tubes creates an aerobic environment.

False

Ortho-nitrophenyl-beta-D-galactopyranoside (ONPG) is structurally similar to lactose and requires lactose permease for entry into the cell membrane.

False

The indole test is used to identify organisms that produce the enzyme tryptophanase, which converts tryptophan into indole, pyruvic acid, and ammonia.

True

IMViC tests include Indole, Methyl Red, Voges-Proskauer, and Urease reactions.

False

In Lysine iron agar (LIA) slant, the presence of black precipitate indicates a negative H2S production result.

False

In TSI agar, a red slant and yellow butt indicate that the organism is capable of both oxidation and fermentation.

False

A positive result for citrate utilization in the Oxidation-Fermentation Test is indicated by a blue color change.

True

If an organism is urease positive, it will show a yellow color change after adding urea.

False

In Nitrate & Nitrite Reduction test, a red coloration indicates that the organism has further reduced nitrite to nitrogen gas.

False

In the IMViC tests, a negative result for indole production is indicated by a purple color change after adding Kovac's reagent.

True

Test your knowledge of oxidation-fermentation (O/F) patterns in bacterial identification using Hugh and Leifson O/F basal medium. Learn to interpret pH indicator changes in the medium, such as yellow for acidic and blue for alkaline environments.

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