Nucleic Acid Extraction Methods in Molecular Biology

What is the purpose of nucleic acid extraction?

To isolate DNA and RNA from the cell for use in subsequent procedures

Which method involves the isolation of mitochondrial DNA?

Inorganic Isolation Methods

In which methods should the target nucleic acid ideally be free of contamination with protein, carbohydrate, lipids, or other nucleic acid?

All of the above

What is used to determine the quantity and quality of DNA and RNA preparations?

All of the above Signup and view all the answers

Which method involves the isolation of polyA (messenger) RNA?

Total RNA Extraction Signup and view all the answers

What is the main purpose of comparing and contrasting different isolation methods?

To understand the advantages and disadvantages of each technique Signup and view all the answers

What was used by Meselson and Stahl in 1958 to demonstrate semiconservative replication of DNA?

Density gradient centrifugation Signup and view all the answers

Which method is used to isolate white blood cells (WBCs) from blood or bone marrow specimens?

Differential density gradient centrifugation Signup and view all the answers

What is the purpose of using Ficoll when isolating white blood cells (WBCs)?

To remove RBCs from the sample Signup and view all the answers

What is the main purpose of alkaline lysis procedures?

To precipitate large chromosomal DNA with proteins Signup and view all the answers

What is the initial step in nucleic acid isolation dependent on?

The nature of the starting material Signup and view all the answers

What does Ficoll settle into during differential density gradient centrifugation of whole blood or bone marrow mixed with isotonic saline?

A layer containing the mononuclear WBCs Signup and view all the answers

What is the purpose of incubating whole blood or bone marrow in hypotonic buffer?

To take advantage of the differences in the osmotic fragility of RBCs and WBCs Signup and view all the answers

What was first isolated from human cells by Miescher in 1869?

Chromosomal DNA Signup and view all the answers

'Large chromosomal DNA and proteins cannot renature properly when neutralized in acetate at low pH after alkaline treatment'. What does this lead to?

'Large aggregates' with proteins Signup and view all the answers

'Ficoll is a highly branched sucrose polymer that does not penetrate biological membranes'. What is this characteristic utilized for?

'Mononuclear' WBCs to settle into a layer below plasma components Signup and view all the answers