Ninhydrin and Biuret Tests for Amino Acids and Proteins

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42 Questions

What is the main functional group responsible for the reduction of metal ions in reducing sugars?

Enediol

Which test is used to differentiate between reducing and non-reducing sugars?

Benedict's Test

What is the characteristic structure of an osazone when viewed under a microscope?

Crystalline

What is the result of the reaction between a reducing sugar and copper (II) ions in Benedict's Test?

Reduction of copper (II) ions to copper (I) ions

Which of the following sugars does not have a free carbonyl group?

Cellulose

What is the anionic form of enediol that carries a negative charge?

Enediolate

Which of the following sugars is a non-reducing sugar?

Sucrose

What is the primary component of starch in plants?

Glucose

Which test is specific for fructose in the presence of glucose?

Indole-3-acetic acid test

What is the purpose of Fehling's Test?

To differentiate between reducing and non-reducing sugars

Which of the following sugars is not a reducing sugar?

Sucrose

What is the temperature range required for the indole-3-acetic acid test?

40-50°C

What is the primary purpose of the Biuret test?

To detect the presence of amino acids or proteins

What is the positive indication of the Sakaguchi's test?

Red color

Which test is used to detect the presence of aromatic amino acids?

Ehlrich's test

What is the result of the Xanthoproteic test on casein?

Positive

What is the effect of heat on proteins?

Denaturation

Which reagents are used in the Hopkins-Cole reaction?

Lead acetate and glyoxylic acid

What is the result of the Ninhydrin test on arginine?

Positive

What is the result of the Lead Acetate test on cysteine?

Black precipitate

What is the result of the Nitroprusside reaction on cysteine?

Positive

Which test is used to detect the presence of peptides/proteins?

Pauly's test

Which of the following tests is used to differentiate aldoses and ketoses?

Seliwanoff test

What is the purpose of the Molisch test?

To detect the presence of carbohydrates

What is the color produced by the Molisch test in the presence of carbohydrates?

Purple or red-violet

What is the purpose of the Anthrone test?

To detect the presence of carbohydrates

Which of the following compounds produces a cherry red color in the Seliwanoff test?

Fructose

What is the purpose of Bial's test?

To differentiate pentoses and hexoses

What is the result of the Molisch test with starch?

Negative, no color change

What is the result of the Anthrone test with glucose?

Positive, purple-red color

What is the purpose of the Tollen's phloroglucinol test?

To detect the presence of pentoses and other compounds that can yield furfural derivatives

What is the result of the Seliwanoff test with arabinose?

Positive, green color

What is the primary purpose of the Mucic Acid Test?

To detect the presence of galactose

What is the characteristic of soap that allows it to act as a surfactant?

Its hydrophilic and hydrophobic contents

What is the purpose of the Rosenheim's Test for Choline?

To detect the presence of choline, phospholipids, cerebrosides, and sphingomyelins

What is the result of the Saponification reaction?

The formation of soap and glycerol

What is the purpose of the Solubility Test?

To determine the solubility of fats and oils

What is the characteristic of an insoluble soap?

It is water insoluble and oil soluble

What is the purpose of the Lieberman-Burchard Test?

To detect the presence of cholesterol

What is the result of the Salting Out reaction?

The formation of a precipitate

What is the purpose of the Iodine Test?

To distinguish between saturated and unsaturated fatty acids

What is the characteristic of a sample that yields a positive result in the Mucic Acid Test?

It contains galactose

This quiz covers the principles and results of Ninhydrin and Biuret tests, which are used to detect the presence of amino acids and proteins. Learn about the ingredients, indications, and results of these tests. Practice identifying the tests' positive and negative results.

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