Podcast
Questions and Answers
What is a primary reason these organisms are not commonly cultured in clinical laboratories?
What is a primary reason these organisms are not commonly cultured in clinical laboratories?
- They require specialized equipment that is not available.
- They have fastidious growth requirements. (correct)
- They are highly pathogenic and considered dangerous.
- They are too expensive to maintain.
Which statement best describes the nature of the organisms mentioned?
Which statement best describes the nature of the organisms mentioned?
- They require specific growth conditions that are difficult to replicate. (correct)
- They are resistant to antibiotics and can grow in any medium.
- They thrive in a wide range of environments.
- They can be easily cultured using standard laboratory techniques.
What does the term 'fastidious' imply regarding the growth of these organisms?
What does the term 'fastidious' imply regarding the growth of these organisms?
- They do not have any specific growth requirements.
- They can adapt to a variety of growth mediums.
- They need precise environmental conditions for optimal growth. (correct)
- They grow rapidly and require minimal resources.
Which of the following might be a consequence of the fastidious growth requirements of these organisms?
Which of the following might be a consequence of the fastidious growth requirements of these organisms?
Why might specialized laboratories be necessary for culturing these organisms?
Why might specialized laboratories be necessary for culturing these organisms?
Which of the following treatments is ineffective against M. pneumoniae?
Which of the following treatments is ineffective against M. pneumoniae?
Which of the following Mycoplasma species is NOT a genital type?
Which of the following Mycoplasma species is NOT a genital type?
Which method is primarily used for detecting specific antibodies?
Which method is primarily used for detecting specific antibodies?
What is the correct list of treatments for M. pneumoniae?
What is the correct list of treatments for M. pneumoniae?
Which of the following options correctly identifies the three genital Mycoplasma species?
Which of the following options correctly identifies the three genital Mycoplasma species?
Flashcards
Fastidious Organisms
Fastidious Organisms
These organisms have very specific and demanding growth conditions.
Why not grow them in the lab?
Why not grow them in the lab?
Fastidious organisms are not commonly grown in clinical labs.
Clinical Laboratory
Clinical Laboratory
Clinical labs are the places where patient samples are tested and analyzed.
Difficult to culture
Difficult to culture
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Why are fastidious organisms not routinely cultured?
Why are fastidious organisms not routinely cultured?
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ELISA
ELISA
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Mycoplasma pneumoniae
Mycoplasma pneumoniae
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Treatment for Mycoplasma pneumoniae
Treatment for Mycoplasma pneumoniae
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Mycoplasma
Mycoplasma
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Genital Mycoplasma Species
Genital Mycoplasma Species
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Study Notes
Mycoplasma and Ureaplasma
- Mycoplasmas are small organisms (0.2-0.3 µm)
- Lack cell walls (no peptidoglycan)
- Pleomorphic shape (neither rod nor coccus)
- Not visible with Gram stain
- Cell membranes contain sterols
- Insensitive to antibiotics that inhibit cell division (e.g., penicillin)
- Species commonly found in the mouth and genitourinary (GU) tracts, some cause disease.
Mycoplasma pneumoniae
- Causes atypical pneumonia (20% of cases)
- Transmitted via respiratory droplets
- Symptoms differ from typical lobar pneumonia
- Can also cause bronchitis, pharyngitis, and otitis media
- Worldwide distribution, increased incidence in late summer/early fall
- Adheres to host cells via a protein (P1) that binds sialic acid
- Inhibits ciliary action, leading to inflammation and tissue damage
Genital Mycoplasmas
- Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma urealyticum are urogenital pathogens
- Often associated with sexually transmitted infections (STIs)
- Can cause urethritis, pelvic inflammatory disease (PID)
- M. hominis degrades arginine
- U. urealyticum hydrolyzes urea
- Postpartum or post-abortion fever is a major clinical condition associated with M. hominis
Diagnosis and Treatment
- Difficult to isolate Mycoplasma species due to slow growth.
- Nucleic acid amplification tests are more common.
- Treatment options for M. pneumoniae include doxycycline, azithromycin, or levofloxacin.
- Azithromycin is often prescribed for M. genitalium, but macrolide and quinolone resistance is a concern.
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