Mycoplasma and Ureaplasma Quiz
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Questions and Answers

What is a characteristic of Mycoplasma pneumoniae that distinguishes it from other Mycoplasmataceae species?

  • It replicates exclusively by binary fission.
  • It is the only anaerobic organism in its family.
  • It requires no sterols for growth.
  • It is capable of gliding motility. (correct)

Which organism is primarily associated with the cause of primary atypical pneumonia?

  • Mycoplasma hominis
  • Mycoplasma pneumoniae (correct)
  • Ureaplasma urealyticum
  • Mycoplasma fermentans

What is the expected appearance of Mycoplasma colonies when cultured?

  • Fried egg appearance (correct)
  • Rod-shaped clusters
  • Fuzzy and irregular margins
  • Circular and transparent

In which situation can Mycoplasma hominis be transmitted to infants?

<p>During birth (B)</p> Signup and view all the answers

What distinguishes Ureaplasma parvum from Ureaplasma urealyticum?

<p>Biovar classification (A)</p> Signup and view all the answers

What is the primary purpose of the manganese chloride urea test?

<p>To rapidly identify Ureaplasma urealyticum (C)</p> Signup and view all the answers

Which culture method is specifically required for the growth of Mycoplasma pneumoniae?

<p>Biphasic culture system (A)</p> Signup and view all the answers

What characteristic is typical of colonies formed by Mycoplasma hominis on A7 agar?

<p>Pinpoint colonies resembling fried eggs (D)</p> Signup and view all the answers

Which of the following methods is NOT typically used for the diagnosis of Mycoplasma pneumoniae?

<p>Bacterial culture on blood agar plates (B)</p> Signup and view all the answers

What is the main reason why blood culture is not performed in the identification of Ureaplasma urealyticum?

<p>Growth is inhibited by soliven polyanthy sulfonate (C)</p> Signup and view all the answers

Flashcards

Mycoplasma characteristics

Small, cell wall-less bacteria requiring sterols (like cholesterol) for growth, and often parasites of the genital tract.

Mycoplasma pneumoniae role

Causes atypical pneumonia (walking pneumonia), is aerobic, and attaches strongly to respiratory cells.

Mycoplasma hominis infections

A genital mycoplasma species linked to newborn infections (sepsis, meningitis, pneumonia) and other conditions like pelvic inflammatory disease.

Ureaplasma urealyticum infections

Another genital mycoplasma causing non-gonococcal urethritis and chorioamnionitis.

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Mycoplasma diagnostic methods

Cannot be seen using Gram staining, but can be identified via special stains like Dienes Method and culture methods.

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Mycoplasma Culture Media

Specialized media like biphasic pleuropneumonia-like organism broth/agar, A8 agar, Modified New York City Medium, and anaerobic BAP are used to grow Mycoplasma.

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Mycoplasma pneumoniae Culture

Needs a biphasic culture system; incubation up to 3 weeks with 5-10% CO2 is essential.

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Ureaplasma urealyticum Identification

Manganese chloride urea test is used. (+) result shows a dark brown precipitate around colonies. Shepard 10B arginine broth is one type of transport media which needs supplements like arginine and the growth needs to be monitored for color changes (red).

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Mycoplasma spp. Growth Pattern

Mycoplasma species are known for their lack of cell walls. They grow slowly compared to bacteria with cell walls, are pinpoint colonies on certain agar, and often form 'fried egg' colonies on A7.

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Spirillum Characteristics

Spiral-shaped bacteria, possibly anaerobic, often require special microscopy/staining methods like dark-field, immunofluorescence, silver stains, which can view the axial filaments/periplasmic flagella, to be observed.

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