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Questions and Answers
What is the defining characteristic of mycoplasmas?
What is the defining characteristic of mycoplasmas?
What type of bacteria are mycoplasmas classified as?
What type of bacteria are mycoplasmas classified as?
What type of environment are mycoplasmas sensitive to?
What type of environment are mycoplasmas sensitive to?
Which antibiotics are mycoplasmas resistant to?
Which antibiotics are mycoplasmas resistant to?
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What type of existence do mycoplasmas exhibit due to limited biosynthetic capabilities?
What type of existence do mycoplasmas exhibit due to limited biosynthetic capabilities?
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What type of organisms are mycoplasmas in terms of oxygen requirements?
What type of organisms are mycoplasmas in terms of oxygen requirements?
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How many species of Ureaplasma are considered to be of pathological significance?
How many species of Ureaplasma are considered to be of pathological significance?
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What type of growth medium do mycoplasmas require?
What type of growth medium do mycoplasmas require?
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Where is Ureaplasma mainly present?
Where is Ureaplasma mainly present?
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Which of these is not a species of Ureaplasma considered to be of pathological significance?
Which of these is not a species of Ureaplasma considered to be of pathological significance?
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In which type of growth medium are mycoplasmas cultured?
In which type of growth medium are mycoplasmas cultured?
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Which diagnostic method is used for testing mycoplasma by inoculating a cell line?
Which diagnostic method is used for testing mycoplasma by inoculating a cell line?
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What type of disease is not caused by Mycoplasma genitalium?
What type of disease is not caused by Mycoplasma genitalium?
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Which of the following is not one of the diseases caused by Mycoplasma organism?
Which of the following is not one of the diseases caused by Mycoplasma organism?
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Which mycoplasma species is linked to nongonococcal urethritis?
Which mycoplasma species is linked to nongonococcal urethritis?
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What type of specimen is used for testing mycoplasma by direct culture on solid or liquid media?
What type of specimen is used for testing mycoplasma by direct culture on solid or liquid media?
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(Urine samples) are taken in men and women to test for mycoplasma.
(Urine samples) are taken in men and women to test for mycoplasma.
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Which mycoplasma species is associated with pelvic inflammatory disease?
Which mycoplasma species is associated with pelvic inflammatory disease?
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What is the main selective agent in the semi-solid enriched medium for isolating Mycoplasma?
What is the main selective agent in the semi-solid enriched medium for isolating Mycoplasma?
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What is the incubation period required for isolating Mycoplasma using the described culture method?
What is the incubation period required for isolating Mycoplasma using the described culture method?
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Which class of antibiotics is inherently resistant to by all mycoplasmas due to their lack of cell wall?
Which class of antibiotics is inherently resistant to by all mycoplasmas due to their lack of cell wall?
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Which antibiotic class is recommended for treating Mycoplasma pneumoniae infections in older children and adults?
Which antibiotic class is recommended for treating Mycoplasma pneumoniae infections in older children and adults?
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Which of the following supplements the semi-solid enriched medium as a source of cholesterol and other lipids?
Which of the following supplements the semi-solid enriched medium as a source of cholesterol and other lipids?
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What staining method can be used to visualize Mycoplasma colonies in the described culture method?
What staining method can be used to visualize Mycoplasma colonies in the described culture method?
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Study Notes
Characteristics of Mycoplasmas
- Mycoplasmas are defined by their lack of a cell wall.
- They are classified as a type of bacteria.
- They are sensitive to dry environments and require a humid environment to survive.
Resistance to Antibiotics
- Mycoplasmas are resistant to beta-lactam antibiotics due to their lack of cell walls.
Metabolic Capabilities
- Mycoplasmas have limited biosynthetic capabilities and require a rich medium for growth.
- They exhibit a parasitic or saprophytic existence.
Oxygen Requirements
- Mycoplasmas are facultative anaerobes, meaning they can grow in the presence or absence of oxygen.
Ureaplasma
- There are 14 species of Ureaplasma, of which 2 are considered to be of pathological significance.
- Ureaplasma is mainly present in the urogenital tract.
Growth Medium
- Mycoplasmas require a rich, cholesterol-enriched medium for growth.
Culture and Diagnosis
- Mycoplasmas are cultured in a semi-solid enriched medium.
- The diagnostic method used for testing mycoplasma involves inoculating a cell line.
- The incubation period required for isolating Mycoplasma is 2-4 weeks.
Diseases Caused by Mycoplasma
- Mycoplasma genitalium is linked to nongonococcal urethritis.
- Mycoplasma hominis is associated with pelvic inflammatory disease.
- Mycoplasma pneumoniae is not associated with genitourinary disease.
Specimen Collection
- Urine samples are taken from men and women to test for mycoplasma.
Treatment
- Macrolide antibiotics are recommended for treating Mycoplasma pneumoniae infections in older children and adults.
Culture Method
- The semi-solid enriched medium is supplemented with horse serum as a source of cholesterol and other lipids.
- The Dienes staining method can be used to visualize Mycoplasma colonies.
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Description
Test your knowledge about mycoplasmas, a class of bacteria commonly referred to as the smallest free-living organisms known for their lack of cell wall and limited biosynthetic capabilities. Learn about their sensitivity to environmental conditions and antibiotics such as tetracycline and erythromycin.
