Mycoplasma and Ureaplasma Bacteria Quiz

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Questions and Answers

What is the defining characteristic of mycoplasmas?

They are the largest free-living organisms known

They lack a cell wall (correct)

They are resistant to tetracycline and erythromycin

They have a cell wall made of peptidoglycan

What type of bacteria are mycoplasmas classified as?

Mollicutes (correct)

Cyanobacteria

Spirochetes

Gram-positive bacteria

What type of environment are mycoplasmas sensitive to?

High pH

Environmental conditions (correct)

Low humidity

High temperature

Which antibiotics are mycoplasmas resistant to?

Penicillin and amoxicillin Signup and view all the answers

What type of existence do mycoplasmas exhibit due to limited biosynthetic capabilities?

Parasitic or saprophytic Signup and view all the answers

What type of organisms are mycoplasmas in terms of oxygen requirements?

Strict aerobes Signup and view all the answers

How many species of Ureaplasma are considered to be of pathological significance?

Six Signup and view all the answers

What type of growth medium do mycoplasmas require?

Enriched growth medium with nucleic acid precursors, fatty acids, and amino acids Signup and view all the answers

Where is Ureaplasma mainly present?

Urinary and genital tract Signup and view all the answers

Which of these is not a species of Ureaplasma considered to be of pathological significance?

Ureaplasma hominis Signup and view all the answers

In which type of growth medium are mycoplasmas cultured?

Medium containing serum, yeast extract, and penicillin Signup and view all the answers

Which diagnostic method is used for testing mycoplasma by inoculating a cell line?

Inoculation of cell line (vero cell line) Signup and view all the answers

What type of disease is not caused by Mycoplasma genitalium?

Upper respiratory tract disease Signup and view all the answers

Which of the following is not one of the diseases caused by Mycoplasma organism?

Urinary tract infections Signup and view all the answers

Which mycoplasma species is linked to nongonococcal urethritis?

Mycoplasma genitalium Signup and view all the answers

What type of specimen is used for testing mycoplasma by direct culture on solid or liquid media?

Mucous membrane swabbings Signup and view all the answers

(Urine samples) are taken in men and women to test for mycoplasma.

True Signup and view all the answers

Which mycoplasma species is associated with pelvic inflammatory disease?

Mycoplasma hominis Signup and view all the answers

What is the main selective agent in the semi-solid enriched medium for isolating Mycoplasma?

Yeast extract Signup and view all the answers

What is the incubation period required for isolating Mycoplasma using the described culture method?

7-12 days Signup and view all the answers

Which class of antibiotics is inherently resistant to by all mycoplasmas due to their lack of cell wall?

Beta-lactam antibiotics Signup and view all the answers

Which antibiotic class is recommended for treating Mycoplasma pneumoniae infections in older children and adults?

Tetracyclines Signup and view all the answers

Which of the following supplements the semi-solid enriched medium as a source of cholesterol and other lipids?

Human serum Signup and view all the answers

What staining method can be used to visualize Mycoplasma colonies in the described culture method?

Dienes staining Signup and view all the answers

Study Notes

Characteristics of Mycoplasmas

Mycoplasmas are defined by their lack of a cell wall.
They are classified as a type of bacteria.
They are sensitive to dry environments and require a humid environment to survive.

Resistance to Antibiotics

Mycoplasmas are resistant to beta-lactam antibiotics due to their lack of cell walls.

Metabolic Capabilities

Mycoplasmas have limited biosynthetic capabilities and require a rich medium for growth.
They exhibit a parasitic or saprophytic existence.

Oxygen Requirements

Mycoplasmas are facultative anaerobes, meaning they can grow in the presence or absence of oxygen.

Ureaplasma

There are 14 species of Ureaplasma, of which 2 are considered to be of pathological significance.
Ureaplasma is mainly present in the urogenital tract.

Growth Medium

Mycoplasmas require a rich, cholesterol-enriched medium for growth.

Culture and Diagnosis

Mycoplasmas are cultured in a semi-solid enriched medium.
The diagnostic method used for testing mycoplasma involves inoculating a cell line.
The incubation period required for isolating Mycoplasma is 2-4 weeks.

Diseases Caused by Mycoplasma

Mycoplasma genitalium is linked to nongonococcal urethritis.
Mycoplasma hominis is associated with pelvic inflammatory disease.
Mycoplasma pneumoniae is not associated with genitourinary disease.

Specimen Collection

Urine samples are taken from men and women to test for mycoplasma.

Treatment

Macrolide antibiotics are recommended for treating Mycoplasma pneumoniae infections in older children and adults.

Culture Method

The semi-solid enriched medium is supplemented with horse serum as a source of cholesterol and other lipids.
The Dienes staining method can be used to visualize Mycoplasma colonies.

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Description

Test your knowledge about mycoplasmas, a class of bacteria commonly referred to as the smallest free-living organisms known for their lack of cell wall and limited biosynthetic capabilities. Learn about their sensitivity to environmental conditions and antibiotics such as tetracycline and erythromycin.