Mycoplasma Serology Methods
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Questions and Answers

What appearance do colonies of mycoplasmas typically have on agar?

  • Star-shaped
  • Fried egg (correct)
  • Circular
  • Irregular
  • Which method is NOT commonly used for detecting antibodies in mycoplasma infections?

  • Indirect immunofluorescence
  • Western blotting (correct)
  • Complement fixation (CF) tests
  • Hemagglutination inhibition (HI) tests
  • What is the primary treatment option for mycoplasmal pneumonia?

  • Penicillin
  • Tetracycline (correct)
  • Cephalosporin
  • Vancomycin
  • Which age group is most commonly affected by M pneumoniae pneumonia?

    <p>Persons aged 5–20 years</p> Signup and view all the answers

    What is a common clinical symptom of mycoplasmal pneumonia during its onset?

    <p>Lassitude</p> Signup and view all the answers

    Which laboratory finding is typical of M pneumoniae pneumonia?

    <p>Slightly elevated white cell count</p> Signup and view all the answers

    What is a frequent characteristic of the cough associated with M pneumoniae infection?

    <p>Initially nonproductive</p> Signup and view all the answers

    What is a significant risk associated with ureaplasmas when treating with tetracycline?

    <p>Resistance to the antibiotic</p> Signup and view all the answers

    Which species of Mycoplasma is primarily associated with pneumonia?

    <p>Mycoplasma pneumoniae</p> Signup and view all the answers

    What is the primary type of specimen used for diagnosing mycoplasma infections?

    <p>Throat swabs</p> Signup and view all the answers

    Which environment is necessary for culturing mycoplasmas in the laboratory?

    <p>Microaerophilic conditions with 5% CO2</p> Signup and view all the answers

    What type of growth do mycoplasma colonies exhibit on solid media?

    <p>They appear as protoplasmic masses of indefinite shape.</p> Signup and view all the answers

    Mycoplasma genitalium is primarily associated with which type of infections?

    <p>Urethral infections</p> Signup and view all the answers

    Which of the following conditions is Ureaplasma urealyticum known to cause?

    <p>Lung disease in premature infants</p> Signup and view all the answers

    What is the morphological size range of mycoplasma structures?

    <p>50 to 300 nm</p> Signup and view all the answers

    What type of media is necessary for the effective culture of mycoplasmas?

    <p>Media with serum or ascites fluid</p> Signup and view all the answers

    What is the significance of a cold hemagglutinin titer of 1:64 or more in untreated patients?

    <p>It supports the diagnosis of mycoplasma infection.</p> Signup and view all the answers

    Which laboratory testing method is generally performed only in reference laboratories for diagnosing mycoplasma infections?

    <p>Polymerase chain reaction (PCR) assay</p> Signup and view all the answers

    What treatment options are commonly used for clinical improvement in mycoplasma infections?

    <p>Tetracyclines or erythromycins</p> Signup and view all the answers

    What percentage of women with salpingitis have M. hominis isolated from their uterine tubes?

    <p>10%</p> Signup and view all the answers

    Which organism is primarily associated with causing nongonococcal urethritis in men?

    <p>Ureaplasma urealyticum</p> Signup and view all the answers

    In which clinical condition can M. hominis be cultured from the upper urinary tract?

    <p>Pyelonephritis</p> Signup and view all the answers

    What is the characteristic requirement for the growth of Ureaplasma urealyticum?

    <p>10% urea</p> Signup and view all the answers

    What is a common diagnostic method that detects specific antibodies to M. pneumoniae?

    <p>Complement fixation tests</p> Signup and view all the answers

    Study Notes

    Mycoplasmas Overview

    • Over 150 species of cell wall-free bacteria exist, with four being of primary importance in humans.
    • Mycoplasma pneumoniae is linked to pneumonia, joint issues, and can manifest in individuals aged 5-20 years.
    • Mycoplasma hominis is involved in postpartum fever and uterine tube infections.
    • Ureaplasma urealyticum is associated with nongonococcal urethritis and lung disease in low birth weight infants.
    • Mycoplasma genitalium relates to urethral and other infections.

    Colony Appearance

    • Cultures may exhibit a distinctive "fried egg" appearance on agar, with colonies measuring 20-500 μm.

    Serological Diagnostics

    • Antibodies can be detected in infected humans using various methods, including:
      • Complement fixation (CF) tests with glycolipid antigens from cultured mycoplasmas.
      • Hemagglutination inhibition (HI) tests using tanned red cells.
      • Indirect immunofluorescence techniques.
      • Specific tests measuring growth inhibition by antibodies.

    Treatment Approaches

    • Mycoplasmas show resistance to penicillins, cephalosporins, and vancomycin.
    • Tetracyclines and erythromycins are effective both in vitro and in vivo for treating mycoplasmal pneumonia.
    • Some strains of ureaplasmas may be resistant to tetracyclines.

    Mycoplasma pneumoniae and Atypical Pneumonias

    • Typically presents as mild disease with a clinical spectrum from asymptomatic to serious pneumonitis, possibly affecting other systems (neurological, hematologic).
    • Symptoms include insidious onset with fever, headache, sore throat, and cough that may progress to blood-streaked sputum.
    • Incubation period lasts from 1 to 3 weeks.

    Laboratory Tests for Diagnosis

    • Diagnosis relies heavily on clinical recognition; laboratory tests provide secondary support.
    • Slightly elevated white cell count may appear; sputum Gram stain helps rule out other pathogens (e.g., Streptococcus pneumoniae).
    • Mycoplasmas are rarely cultured due to the need for specialized tests.
    • Cold agglutinins appear in about 50% of untreated cases, peaking in the third or fourth week of illness.
    • Specific antibodies can be detected through CF tests with acute and convalescent phase sera.

    Mycoplasma hominis Specifics

    • Associated with a range of diseases, particularly in the urinary tract.
    • Found in 10% of pyelonephritis patients and linked to infections such as salpingitis and tubo-ovarian abscesses.
    • Isolated from blood in women with postabortal or postpartum infections.

    Ureaplasma urealyticum Characteristics

    • Implicated in various diseases, particularly nongonococcal urethritis in men, though Chlamydia trachomatis is the primary cause.
    • Commonly resides in the female genital tract with a weak association to disease.

    Culture and Microscopy

    • Mycoplasma cultures require serum, growth factors, and metabolic substrates for growth.
    • Culture methods involve special solid media incubated under specific conditions for several days.
    • Direct microscopic examination is generally considered ineffective.

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    Description

    This quiz focuses on the serological techniques used to detect antibodies in humans infected with mycoplasmas. It covers various methods such as CF tests, HI tests, and indirect immunofluorescence, and explains their specificity in measuring immune response. Test your understanding of these critical diagnostic approaches!

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