Mycobacteria General Characteristics
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Questions and Answers

Which enzyme deficiency is detected by the test described in the text?

  • Niacinase deficiency
  • Niacin connecting enzyme deficiency (correct)
  • Niacin dehydrogenase deficiency
  • Niacin ribonucleotide reductase deficiency
  • What is the positive result indicator for M.tuberculosis in the described test?

  • Red color
  • Yellow color (correct)
  • Blue color
  • Green color
  • What culture medium should be used in the test for detecting the ability of isolates to reduce nitrate?

  • Eosin Methylene Blue agar
  • Lowenstein-Jensen medium
  • MacConkey agar
  • Nutrient broth with 0.1% potassium nitrate (correct)
  • Which of the following organisms is NOT strongly positive in the nitrate reduction test?

    <p>M.kansasii</p> Signup and view all the answers

    What is a key distinguishing feature of M.tuberculosis in the nitrate reduction test?

    <p>Red coloration</p> Signup and view all the answers

    Which reagent is NOT used in the test to detect nitrate reduction ability?

    <p>Cyanogen bromide</p> Signup and view all the answers

    What does a positive result in the nitrate reduction test indicate about an organism's ability?

    <p>Reducing nitrate to nitrogen gas</p> Signup and view all the answers

    Which species serves as a quality control positive agent for the niacin detection test?

    <p>M.fortuitum ATCC 6841</p> Signup and view all the answers

    What is the role of Zinc in the nitrate reduction test?

    <p>Indicator reagent to detect nitrate or true negative results</p> Signup and view all the answers

    Which organism shows accumulation of niacin in the medium as a positive result?

    <p>M.terrae complex</p> Signup and view all the answers

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