12 Questions
What was the first cloning vector described?
Cloning vector in 1977
Which technique revolutionized the production of specific DNA sequences?
Polymerase chain reaction
What determines an organism's characteristics?
The sequence of nucleotides in its DNA
Why are different words formed from the same letters?
The letters are arranged differently
How much of the human DNA sequence is identical between individuals?
99.9%
What was a limitation of the first cloning vector described in 1977?
All of the above
What is the approximate difference in nucleotides between individuals?
3 million nucleotides
What was the initial approach to sequence genetic code?
Techniques borrowed from analytical chemistry
What was the first whole nucleic acid sequence published?
Alanine tRNA of yeast
What technique did Fred Sanger and colleagues develop?
Detecting radiolabelled partial digestion fragments
What was the next breakthrough in sequencing genetic code?
Filling in ends with radioactive nucleotides and using DNA polymerase
What was the final adaptation in sequencing genetic code?
Using a single method for reading sequences with greater resolution
Test your knowledge on the history and techniques of molecular cloning, focusing on its role in the development of protein therapeutics. Learn about the first cloning vector described in 1977 and the advancements made in this field over time.
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