Molecular Cloning and Protein Therapeutics Quiz

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12 Questions

What was the first cloning vector described?

Cloning vector in 1977

Which technique revolutionized the production of specific DNA sequences?

Polymerase chain reaction

What determines an organism's characteristics?

The sequence of nucleotides in its DNA

Why are different words formed from the same letters?

The letters are arranged differently

How much of the human DNA sequence is identical between individuals?

99.9%

What was a limitation of the first cloning vector described in 1977?

All of the above

What is the approximate difference in nucleotides between individuals?

3 million nucleotides

What was the initial approach to sequence genetic code?

Techniques borrowed from analytical chemistry

What was the first whole nucleic acid sequence published?

Alanine tRNA of yeast

What technique did Fred Sanger and colleagues develop?

Detecting radiolabelled partial digestion fragments

What was the next breakthrough in sequencing genetic code?

Filling in ends with radioactive nucleotides and using DNA polymerase

What was the final adaptation in sequencing genetic code?

Using a single method for reading sequences with greater resolution

Test your knowledge on the history and techniques of molecular cloning, focusing on its role in the development of protein therapeutics. Learn about the first cloning vector described in 1977 and the advancements made in this field over time.

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