Molecular Cloning Quiz 6

What is the role of the recombinant plasmid in bacterial cells?

How can the orientation of an insert in a vector be tested?

Which statement is true regarding the color of bacterial cells that acquire a recombinant plasmid?

What is the purpose of ligation in molecular cloning?

What does the compound Xgal do in cloning procedures?

What is the primary function of a restriction enzyme in molecular cloning?

Which of the following is typically used as a vector in bacterial cloning?

What is the process called where a foreign DNA fragment is inserted into a bacterium?

What technique is used to identify bacterial colonies containing a specific construct using a selectable marker?

Which of the following enzymes is responsible for making a DNA copy from RNA?

Which of the following statements is false regarding recombinant plasmids?

During blue-white screening, what indicates the presence of a recombinant plasmid?

What is a key feature of plasmid replication?

True or False Questions (Molecular Cloning)

Cloning always selects blue colonies. False.
Colonies are blue due to disruption of the lacZ gene. True.
The gene of interest should always be in the lacZ gene. True.
Xgal produces blue coloration. True.
Restriction enzymes are used for both cloning and checking insert orientation. True.
The same restriction enzyme is used for both cloning steps. True.

Multiple Choice Questions (Molecular Cloning)

The main function of restriction enzymes is to cut DNA at specific sequences.
Plasmids are typically used as vectors in bacterial cloning.
Transformation is the process of inserting a foreign DNA fragment into bacterial cells.
Blue-white screening identifies bacterial colonies with the desired construct.
Reverse transcriptase creates a DNA copy from RNA.

Molecular Cloning Steps

Insert preparation
Vector preparation
Ligation
Transformation
Colony screening

Molecular Cloning Enzyme Selection

Enzyme B is preferred for cloning because it cuts upstream and downstream of the gene of interest but not within the gene itself.
Enzyme B also cuts a vector only once, within the lacZ gene

Molecular Cloning Restriction Enzyme Orientation Testing

Restriction enzyme C can be used to test the orientation of an insert.
If the insert is correctly oriented, digestion results in fragments of 230 bp and 5650 bp.
Incorrect orientation results in fragments of 650 bp and 5230 bp.

Test your knowledge on molecular cloning with this quiz featuring true or false questions and multiple choice questions. Cover key concepts such as restriction enzymes, plasmids, and the cloning process. Perfect for students studying biotechnology or genetics.