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Molecular Cloning in Biotechnology

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Questions and Answers

What is the purpose of using adaptors in DNA cloning?

To provide sticky ends for ligation with target DNA (correct)

To introduce new restriction sites in the target DNA

To remove restriction sites from the target DNA

To amplify the target DNA sequence

Why are the sticky ends of adaptors modified?

To introduce new restriction sites in the target DNA

To enhance the ligation efficiency with target DNA

To prevent self-ligation of adaptors (correct)

To facilitate the cleavage of adaptors from the target DNA

What enzyme is used to convert the modified 5' terminus of adaptors to a natural form?

Restriction endonuclease

Polynucleotide kinase (correct)

DNA ligase

DNA polymerase

What is a homopolymer?

A strand composed of one type of nucleotide Signup and view all the answers

What is the purpose of using restriction endonucleases in DNA cloning?

To introduce sticky ends in the target DNA Signup and view all the answers

What is the advantage of using adaptors with modified sticky ends?

It prevents self-ligation of adaptors Signup and view all the answers

What is the purpose of using blunt-ended DNA molecules in cloning?

To facilitate the ligation of adaptors to the target DNA Signup and view all the answers

What is the issue that arises if the target DNA also has the same restriction site as the one used for producing sticky ends?

The target DNA will be cleaved into multiple fragments Signup and view all the answers

What is the purpose of using vectors in DNA cloning?

To amplify and propagate the cloned DNA fragment Signup and view all the answers

What is the purpose of homopolymer tailing in DNA cloning?