10 Questions
What is the purpose of using adaptors in DNA cloning?
To provide sticky ends for ligation with target DNA
Why are the sticky ends of adaptors modified?
To prevent self-ligation of adaptors
What enzyme is used to convert the modified 5' terminus of adaptors to a natural form?
Polynucleotide kinase
What is a homopolymer?
A strand composed of one type of nucleotide
What is the purpose of using restriction endonucleases in DNA cloning?
To introduce sticky ends in the target DNA
What is the advantage of using adaptors with modified sticky ends?
It prevents self-ligation of adaptors
What is the purpose of using blunt-ended DNA molecules in cloning?
To facilitate the ligation of adaptors to the target DNA
What is the issue that arises if the target DNA also has the same restriction site as the one used for producing sticky ends?
The target DNA will be cleaved into multiple fragments
What is the purpose of using vectors in DNA cloning?
To amplify and propagate the cloned DNA fragment
What is the purpose of homopolymer tailing in DNA cloning?
To facilitate the ligation of adaptors to the target DNA
Explore the concept of molecular cloning in biotechnology, which involves making identical copies of DNA molecules from different sources. Learn about the process of cloning and its applications in biotechnology.
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