12 Questions
What is the function of restriction/digestion enzymes in molecular cloning?
To cut DNA at specific sites
What is the role of a vector in molecular cloning?
To select a small carrier molecule of DNA capable of self-replication
What is the purpose of ligation in molecular cloning?
To join two DNA fragments covalently
Why do restriction enzymes provide protection to bacteria against invading viruses?
They don't cleave bacterial DNA because it's methylated
What is the main characteristic of the sequences cleaved by restriction enzymes?
They are palindromic
Why are restriction enzymes used mainly in in-vitro experiments?
Because they are very specific in cutting DNA at particular sequences
What is the role of restriction/digestion enzymes in molecular cloning?
Cutting DNA at specific sites
What is the main function of a vector in molecular cloning?
Selecting a small carrier molecule of DNA capable of self-replication
Why are restriction enzymes used mainly in in-vitro experiments?
They cut DNA phosphate-sugar backbone at a very particular sequence
What is the purpose of ligation in molecular cloning?
Joining two DNA fragments covalently forming a recombinant DNA
Why do restriction enzymes provide protection to bacteria against invading viruses?
They cut invading viruses’ DNA into many pieces leaving it inactive inside the bacterial cells
What is the main characteristic of the sequences cleaved by restriction enzymes?
They cut DNA phosphate-sugar backbone at a very particular sequence
Study Notes
Molecular Cloning
Restriction/Digestion Enzymes
- Function in molecular cloning: to cleave DNA molecules at specific recognition sites, producing compatible ends for ligation
- Provide protection to bacteria against invading viruses: by breaking down viral DNA, preventing viral replication
Characteristics
- Recognize and cleave specific DNA sequences: typically 4-8 base pair palindromic sequences
- Used mainly in in-vitro experiments: to manipulate DNA in a controlled environment
Vectors
- Role in molecular cloning: to clone and replicate DNA fragments in a host organism
- Main function: to provide a mechanism for DNA replication and transmission
Ligation
- Purpose in molecular cloning: to join two DNA fragments with compatible ends, forming a recombinant DNA molecule
Test your knowledge of the fundamental requirements for molecular cloning using live cells. Explore topics such as restriction enzymes, vectors, ligation, and moving recombinant DNA into host cells.
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