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Molecular Biotechnology II Lecture 1: Molecular Cloning
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Molecular Biotechnology II Lecture 1: Molecular Cloning

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Questions and Answers

What will happen if the target DNA also possesses the same Restriction Site as the adaptors?

  • The adaptors will produce new DNA with modified ends
  • The adaptors will not be able to bind to the target DNA
  • The adaptors will bind only to the target DNA
  • The adaptors' sticky ends will bind with each other (correct)

    • Why is DNA ligase unable to form a phosphodiester bridge between the 5-OH and 3-OH ends of adaptors?

  • The adaptors cannot bind to DNA molecules
  • The adaptors lack phosphate groups (correct)
  • The adaptors' ends are not complementary
  • The adaptors have abnormal termini

    • What enzyme is used to convert the abnormal 5-OH terminus of adaptors to the natural 5-P form?

  • RNA ligase
  • Polynucleotide kinase (correct)
  • DNA polymerase
  • Reverse transcriptase

    • Why are adaptors unable to ligate with themselves?

    <p>Their sticky ends bind in an unstable manner</p> Signup and view all the answers

    What is the purpose of homopolymer tailing (HT) in the context of DNA manipulation?

    <p>To produce a sticky-ended fragment for vector insertion</p> Signup and view all the answers

    What would be the consequence if DNA ligase was able to bridge between the 5-OH and 3-OH ends of adaptors?

    <p>Adaptors would self-ligate successfully</p> Signup and view all the answers

    Why is it essential that the abnormal 5-OH terminus of adaptors is converted to the natural 5-P form before vector insertion?

    <p>To prevent adaptor self-ligation</p> Signup and view all the answers

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