Molecular Biology Quiz

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Questions and Answers

What is the correct description of the central dogma?

  • The flow of information is from RNA to DNA.
  • Information flows from DNA to mRNA and is then translated into protein. (correct)
  • Central dogma has no significance in molecular biology.
  • It describes the synthesis of RNA from RNA.

Which statement about reverse transcription in retroviruses is correct?

  • It follows the central dogma of molecular biology.
  • It occurs exclusively in eukaryotic organisms.
  • Retroviruses do not utilize reverse transcription.
  • It results in the production of more DNA from RNA. (correct)

Which RNA polymerase is responsible for the transcription of tRNA, 5S rRNA, and snRNAs?

  • RNA polymerase I
  • RNA polymerase II
  • RNA polymerase III (correct)
  • RNA polymerase IV

What is the significance of the 5' → 3' direction in DNA replication?

<p>Nucleotides can only be added to the 3' end of the growing DNA strand. (D)</p> Signup and view all the answers

Which enzymes are primarily involved in the joining of discontinuously synthesized fragments during DNA replication?

<p>DNA ligase (A)</p> Signup and view all the answers

Which chromosome has the most genes?

<p>Chromosome 1 (A)</p> Signup and view all the answers

What is the primary purpose of DNA fingerprinting?

<p>To compare DNA sequences of individuals (D)</p> Signup and view all the answers

Who was the first to create DNA fingerprinting technology?

<p>Alec Jeffrey (B)</p> Signup and view all the answers

What are SNPs in the context of genetics?

<p>Single nucleotide polymorphisms (C)</p> Signup and view all the answers

Which structure did Watson and Crick discover?

<p>Double helix (B)</p> Signup and view all the answers

What kind of DNA is primarily examined in DNA fingerprinting?

<p>Repetitive DNA sequences (B)</p> Signup and view all the answers

Which technique was used by Maurice Wilkins and Rosalind Franklin to study DNA?

<p>X-ray crystallography (B)</p> Signup and view all the answers

What underlies the genetic mapping of the human genome?

<p>Variation in DNA sequence (D)</p> Signup and view all the answers

Which factor is crucial for the initiation of transcription?

<p>Rho factor (A), Sigma factor (C)</p> Signup and view all the answers

Which RNA polymerase is responsible for transcribing rRNA?

<p>RNA polymerase I (A)</p> Signup and view all the answers

What does the term 'aninoacylation of tRNA' refer to?

<p>Linkage of amino acids to their cognate tRNA (A), Charging of tRNA (C)</p> Signup and view all the answers

Which cellular structure is primarily responsible for protein synthesis?

<p>Ribosome (C)</p> Signup and view all the answers

What is one of the initial steps in the process of translation?

<p>The small subunit encounters an mRNA (B)</p> Signup and view all the answers

What is the significance of SNPs?

<p>They represent variations in a single nucleotide (D)</p> Signup and view all the answers

Who developed the technique of DNA fingerprinting?

<p>Alec Jeffreys (A)</p> Signup and view all the answers

What does the process of protein polymerization involve?

<p>Linking of amino acids to form a peptide chain (D)</p> Signup and view all the answers

What is the primary difference between euchromatin and heterochromatin?

<p>Euchromatin is transcriptionally more active than heterochromatin. (A)</p> Signup and view all the answers

Which statement correctly describes the process of DNA replication?

<p>The strands synthesized discontinuously are joined by DNA ligase. (D)</p> Signup and view all the answers

What are regions in DNA where replication begins called?

<p>Origins of replication (D)</p> Signup and view all the answers

In which phase of the cell cycle does DNA replication take place in eukaryotes?

<p>S phase (B)</p> Signup and view all the answers

What was the significance of Frederick Griffith's experiments with Streptococcus?

<p>He demonstrated the miraculous transformation of bacteria. (C)</p> Signup and view all the answers

During transcription, what role does DNA play?

<p>It serves as a template to synthesize RNA. (B)</p> Signup and view all the answers

How does the copying process differ between DNA replication and transcription?

<p>Only a segment of DNA is transcribed during transcription. (D)</p> Signup and view all the answers

What feature characterizes heterochromatin in a nucleus?

<p>It is transcriptionally inactive. (A)</p> Signup and view all the answers

What structure is formed when DNA wraps around histone proteins?

<p>Nucleosome (B)</p> Signup and view all the answers

What did Francis Crick propose in relation to molecular biology?

<p>The central dogma (B)</p> Signup and view all the answers

Which of the following correctly identifies the sequence of elements in a transcription unit?

<p>Promoter - Gene - Terminator (A)</p> Signup and view all the answers

What is the role of AUG in protein synthesis?

<p>It initiates protein synthesis. (C)</p> Signup and view all the answers

What is a cistron?

<p>A sequence of DNA that quotes for a protein. (D)</p> Signup and view all the answers

Which type of mutation is responsible for sickle cell anemia?

<p>Point mutation (C)</p> Signup and view all the answers

What is the functional unit of inheritance according to Mendelian genetics?

<p>Gene (A)</p> Signup and view all the answers

Which of the following describes the relationship between bacteriophages and the copying of DNA strands?

<p>Bacteriophages only replicate one strand of DNA. (A)</p> Signup and view all the answers

What is the primary function of tRNA during protein synthesis?

<p>To carry amino acids and read the genetic code (D)</p> Signup and view all the answers

What interruption occurs in the coding sequence of a gene?

<p>Introns (D)</p> Signup and view all the answers

Which type of RNA has a cloverleaf structure?

<p>tRNA (D)</p> Signup and view all the answers

What role does the ribosome play during translation?

<p>It facilitates the formation of polypeptides from amino acids (A)</p> Signup and view all the answers

Which enzyme is responsible for synthesizing rRNA?

<p>RNA polymerase I (C)</p> Signup and view all the answers

What is the term for the process of adding amino acids to tRNA?

<p>Aminoacylation (A)</p> Signup and view all the answers

What is the function of rRNA in the ribosome?

<p>It catalyzes the formation of peptide bonds (D)</p> Signup and view all the answers

In the context of transcription initiation, what is the role of the sigma factor?

<p>To assist RNA polymerase in recognizing promoter regions (B)</p> Signup and view all the answers

Flashcards

Repetitive sequences

DNA sequences that are repeated multiple times, often hundreds to thousands of times.

Repetitive DNA

Changes in a specific section of a DNA sequence where a tiny length of DNA is repeated multiple times.

Satellite DNA

A type of repetitive DNA categorized by its base composition, segment length, and number of repeating units.

Single Nucleotide Polymorphism (SNP)

Variations in DNA sequence that occur at a single nucleotide, used in genetic mapping and fingerprinting.

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DNA fingerprinting

A technique using changes in repetitive DNA sequences to identify individuals.

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Variable Number of Tandem Repeats (VNTR)

A type of repetitive DNA with a variable number of tandem repeats, used in DNA fingerprinting.

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Self-replication of DNA

The ability of DNA to direct its own replication, ensuring inheritance of traits.

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Constituents of chromosomes

The two components of chromosomes, proteins and DNA, responsible for carrying genetic information.

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Central Dogma

The process where genetic information flows from DNA to RNA and then to proteins.

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DNA Polymerase Direction

DNA-dependent DNA polymerases are enzymes that create new DNA strands using existing DNA as a template. They can only add nucleotides to the 3' end of a growing strand, resulting in a 5' to 3' direction of synthesis.

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tRNA Function

tRNA is a type of RNA that carries amino acids to the ribosome for protein synthesis.

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rRNA function

rRNA is a type of RNA that forms part of the ribosome. It plays a critical role in decoding the genetic information present in mRNA and assembling amino acids into proteins.

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snRNA function

snRNAs are small RNA molecules that function in the splicing of pre-mRNA into mature mRNA in eukaryotic cells.

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Sigma factor

An initiation factor required for the initiation of transcription in bacteria. It helps RNA polymerase bind to the promoter region of DNA.

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Transcription

The process of synthesizing RNA from a DNA template, using RNA polymerase.

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RNA Polymerase I

The enzyme responsible for synthesizing rRNA, a key component of ribosomes.

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Translation

The process of synthesizing a protein from an mRNA template, using ribosomes.

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Aminoacylation of tRNA

The process of attaching an amino acid to its corresponding tRNA molecule, using ATP as energy.

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Ribosome

The cellular organelle responsible for protein synthesis.

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Euchromatin

A form of chromatin that is loosely packed, stains light, and is transcriptionally active.

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Heterochromatin

A form of chromatin that is densely packed, stains dark, and is transcriptionally inactive.

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Origin of Replication

The specific region of DNA where replication begins.

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DNA Ligation

The joining of newly synthesized DNA fragments by DNA ligase.

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S-phase

The phase of the cell cycle where DNA replication occurs.

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Transformation

The transfer of genetic material from one bacterium to another.

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Cistron

A segment of DNA that codes for a specific protein. It represents a functional unit of inheritance, also known as a 'factor' in Mendelian genetics.

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Promoter

A sequence of DNA that signals the start of a gene. It provides a binding site for RNA polymerase, the enzyme responsible for transcribing DNA into RNA.

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Terminator

A sequence of DNA that signals the end of a gene. It indicates where transcription should stop.

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Nucleosome

The basic unit of DNA packaging in eukaryotic cells. It consists of DNA wrapped around a core of eight histone proteins.

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Point Mutation

A type of mutation that involves a change in a single nucleotide within a gene. This can lead to changes in the amino acid sequence of a protein, which may or may not have a significant impact on its function.

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Sickle cell anemia

A genetic disorder caused by a point mutation in the gene for the beta-globin chain of hemoglobin. This mutation results in a change in the amino acid sequence of the protein, causing red blood cells to become sickle-shaped and less efficient at carrying oxygen.

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What is tRNA?

tRNA is a type of RNA that acts as an adaptor molecule during protein synthesis, carrying specific amino acids to the ribosome based on the genetic code.

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What are exons?

Exons are the coding regions of a gene, containing the instructions for building proteins. They are separated by non-coding regions called introns.

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Why are all three types of RNA needed?

All three types of RNA (mRNA, tRNA, and rRNA) are essential for protein synthesis in bacteria and all living organisms.

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What role does each RNA play in translation?

mRNA provides the genetic blueprint, tRNA brings the amino acids, and rRNA serves as the framework and catalyst for protein assembly.

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What does RNA polymerase do?

RNA polymerase is the enzyme responsible for synthesizing RNA from a DNA template. It uses nucleoside triphosphates as building blocks.

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What is the role of the sigma factor?

The sigma factor is a protein that helps RNA polymerase recognize and bind to the promoter region of a gene, initiating transcription.

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What does RNA polymerase I do?

RNA polymerase I is the enzyme responsible for synthesizing rRNA, a key component of ribosomes.

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What is hnRNA?

hnRNA is a precursor molecule for mRNA. It is processed to remove introns and add a cap and tail before becoming mature mRNA.

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Study Notes

  • Nucleic acids are unique in their ability to direct their own replication. Offspring traits depend on precise DNA replication and transmission.
  • DNA is the primary genetic material in most organisms, while RNA also serves as genetic material in some viruses.
  • DNA is a polymer of deoxyribonucleotides, with a base pair defining a nucleotide pair.
  • A nucleotide comprises a nitrogenous base, a pentose sugar (deoxyribose in DNA), and a phosphate group.
  • Purines (Adenine and Guanine) and Pyrimidines (Cytosine, Uracil, and Thymine) are the nitrogenous bases.
  • Cytosine is found in both DNA and RNA; Thymine is in DNA; Uracil is in RNA.
  • A nucleoside is a nitrogenous base linked to a pentose sugar via an N-glycosidic bond.
  • A nucleotide is a nucleoside with one or more phosphate groups linked to the 5'-OH of the nucleoside via phosphodiester linkages.
  • Two nucleotides are linked by a 3'-5' phosphodiester bond, creating a polynucleotide chain.
  • DNA is a double helix; two chains run in opposite directions (antiparallel).
  • Bases are paired via hydrogen bonds; A-T (2 bonds) and G-C (3 bonds).
  • The double helix's stability is enhanced by base-pairing interactions.
  • DNA replication is semiconservative. Each daughter molecule has one original and one new strand.
  • DNA replication requires enzyme DNA polymerase.
  • DNA replication proceeds in one direction (5' to 3') during the process of continuous replication.
  • DNA replication occurs at replication forks.
  • Replication is initiated with the help of helicase enzyme.
  • DNA polymerase is needed for DNA replication and it catalyzes the polymerization process from 5' to 3' on a template strand.
  • Replication in one strand is continuous while in the other strand it's discontinuous forming Okazaki fragments, which are joined by DNA ligase.
  • Replication of DNA takes place at the S phase of the cell cycle.
  • Transcription is the process of copying genetic information from one strand of DNA into RNA, where only one segment of DNA is copied.
  • RNA is made up of ribonucleotides containing RNA.
  • mRNA acts as a template; tRNA carries amino acids and rRNA performs structural and catalytic functions.
  • The mRNA serves as a template; tRNA transports amino acids and reads the genetic information; rRNA performs structural and catalytic functions during translation.
  • The primary transcript is non-functional and contains both exons and introns, undergoing splicing to delete introns.
  • In transcription, the promoter positioned at the 5' end recruits RNA polymerase.
  • The terminator at the 3' end halts transcription, aided by rho factor.
  • A cistron is a segment of DNA coding for a polypeptide.
  • DNA is the primary genetic material in most organisms.
  • The Central Dogma of molecular biology states that genetic information flows from DNA to RNA to protein.
  • Proof that DNA is the genetic material was given by Hershey and Chase.
  • Histones are rich in arginine and lysine and are positively charged proteins.
  • A single nucleosome includes around 200 base pairs.
  • Chromatin is the nucleosome's repeating unit.
  • Heterochromatin is densely packed chromatin; euchromatin is loosely packed and transcriptionally active.
  • DNA replicates semi-conservatively.
  • The process of copying genetic information from DNA to RNA is called transcription.
  • Genetic code is triplet, degenerate, and universal.
  • A codon specifies an amino acid; three codons (stop codons) do not specify amino acids.
  • Translation is the process of protein synthesis, transferring the genetic information from mRNA to a polypeptide.
  • tRNA acts as an 'adapter' molecule to match mRNA codons to amino acids.
  • rRNA plays structural and catalytic roles in protein synthesis.
  • Griffith's experiment demonstrated bacterial transformation, showing that some substance from dead bacteria is transferred to live bacteria.
  • Avery, MacLeod, and McCarty identified DNA as the transforming principle.
  • Hershey and Chase proved that DNA is the genetic material of bacteriophages.
  • Mutations alter genetic code, leading to changes in proteins and phenotypes.
  • Some mutations, such as point mutations, are single base pair alterations.
  • Insertion or deletion of multiple bases causes frame shift mutations.
  • The Human Genome Project's aim was to map the entire human genetic sequence (genome).
  • The genome contains 3.2 billion bases, with 20,000-25,000 genes.
  • DNA fingerprinting (using SNPs) is employed for identifying individuals due to the differences in DNA sequences.
  • The Central Dogma of molecular biology holds that genetic information flows from DNA to RNA to protein.
  • Regulation of gene expression controls when and how genes are expressed.
  • The lac operon controls the expression of genes involved in lactose metabolism.
  • Genes are turned 'on' or 'off', thus regulated, when certain functions begin or cease.

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