Podcast
Questions and Answers
What is the correct description of the central dogma?
What is the correct description of the central dogma?
- The flow of information is from RNA to DNA.
- Information flows from DNA to mRNA and is then translated into protein. (correct)
- Central dogma has no significance in molecular biology.
- It describes the synthesis of RNA from RNA.
Which statement about reverse transcription in retroviruses is correct?
Which statement about reverse transcription in retroviruses is correct?
- It follows the central dogma of molecular biology.
- It occurs exclusively in eukaryotic organisms.
- Retroviruses do not utilize reverse transcription.
- It results in the production of more DNA from RNA. (correct)
Which RNA polymerase is responsible for the transcription of tRNA, 5S rRNA, and snRNAs?
Which RNA polymerase is responsible for the transcription of tRNA, 5S rRNA, and snRNAs?
- RNA polymerase I
- RNA polymerase II
- RNA polymerase III (correct)
- RNA polymerase IV
What is the significance of the 5' → 3' direction in DNA replication?
What is the significance of the 5' → 3' direction in DNA replication?
Which enzymes are primarily involved in the joining of discontinuously synthesized fragments during DNA replication?
Which enzymes are primarily involved in the joining of discontinuously synthesized fragments during DNA replication?
Which chromosome has the most genes?
Which chromosome has the most genes?
What is the primary purpose of DNA fingerprinting?
What is the primary purpose of DNA fingerprinting?
Who was the first to create DNA fingerprinting technology?
Who was the first to create DNA fingerprinting technology?
What are SNPs in the context of genetics?
What are SNPs in the context of genetics?
Which structure did Watson and Crick discover?
Which structure did Watson and Crick discover?
What kind of DNA is primarily examined in DNA fingerprinting?
What kind of DNA is primarily examined in DNA fingerprinting?
Which technique was used by Maurice Wilkins and Rosalind Franklin to study DNA?
Which technique was used by Maurice Wilkins and Rosalind Franklin to study DNA?
What underlies the genetic mapping of the human genome?
What underlies the genetic mapping of the human genome?
Which factor is crucial for the initiation of transcription?
Which factor is crucial for the initiation of transcription?
Which RNA polymerase is responsible for transcribing rRNA?
Which RNA polymerase is responsible for transcribing rRNA?
What does the term 'aninoacylation of tRNA' refer to?
What does the term 'aninoacylation of tRNA' refer to?
Which cellular structure is primarily responsible for protein synthesis?
Which cellular structure is primarily responsible for protein synthesis?
What is one of the initial steps in the process of translation?
What is one of the initial steps in the process of translation?
What is the significance of SNPs?
What is the significance of SNPs?
Who developed the technique of DNA fingerprinting?
Who developed the technique of DNA fingerprinting?
What does the process of protein polymerization involve?
What does the process of protein polymerization involve?
What is the primary difference between euchromatin and heterochromatin?
What is the primary difference between euchromatin and heterochromatin?
Which statement correctly describes the process of DNA replication?
Which statement correctly describes the process of DNA replication?
What are regions in DNA where replication begins called?
What are regions in DNA where replication begins called?
In which phase of the cell cycle does DNA replication take place in eukaryotes?
In which phase of the cell cycle does DNA replication take place in eukaryotes?
What was the significance of Frederick Griffith's experiments with Streptococcus?
What was the significance of Frederick Griffith's experiments with Streptococcus?
During transcription, what role does DNA play?
During transcription, what role does DNA play?
How does the copying process differ between DNA replication and transcription?
How does the copying process differ between DNA replication and transcription?
What feature characterizes heterochromatin in a nucleus?
What feature characterizes heterochromatin in a nucleus?
What structure is formed when DNA wraps around histone proteins?
What structure is formed when DNA wraps around histone proteins?
What did Francis Crick propose in relation to molecular biology?
What did Francis Crick propose in relation to molecular biology?
Which of the following correctly identifies the sequence of elements in a transcription unit?
Which of the following correctly identifies the sequence of elements in a transcription unit?
What is the role of AUG in protein synthesis?
What is the role of AUG in protein synthesis?
What is a cistron?
What is a cistron?
Which type of mutation is responsible for sickle cell anemia?
Which type of mutation is responsible for sickle cell anemia?
What is the functional unit of inheritance according to Mendelian genetics?
What is the functional unit of inheritance according to Mendelian genetics?
Which of the following describes the relationship between bacteriophages and the copying of DNA strands?
Which of the following describes the relationship between bacteriophages and the copying of DNA strands?
What is the primary function of tRNA during protein synthesis?
What is the primary function of tRNA during protein synthesis?
What interruption occurs in the coding sequence of a gene?
What interruption occurs in the coding sequence of a gene?
Which type of RNA has a cloverleaf structure?
Which type of RNA has a cloverleaf structure?
What role does the ribosome play during translation?
What role does the ribosome play during translation?
Which enzyme is responsible for synthesizing rRNA?
Which enzyme is responsible for synthesizing rRNA?
What is the term for the process of adding amino acids to tRNA?
What is the term for the process of adding amino acids to tRNA?
What is the function of rRNA in the ribosome?
What is the function of rRNA in the ribosome?
In the context of transcription initiation, what is the role of the sigma factor?
In the context of transcription initiation, what is the role of the sigma factor?
Flashcards
Repetitive sequences
Repetitive sequences
DNA sequences that are repeated multiple times, often hundreds to thousands of times.
Repetitive DNA
Repetitive DNA
Changes in a specific section of a DNA sequence where a tiny length of DNA is repeated multiple times.
Satellite DNA
Satellite DNA
A type of repetitive DNA categorized by its base composition, segment length, and number of repeating units.
Single Nucleotide Polymorphism (SNP)
Single Nucleotide Polymorphism (SNP)
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DNA fingerprinting
DNA fingerprinting
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Variable Number of Tandem Repeats (VNTR)
Variable Number of Tandem Repeats (VNTR)
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Self-replication of DNA
Self-replication of DNA
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Constituents of chromosomes
Constituents of chromosomes
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Central Dogma
Central Dogma
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DNA Polymerase Direction
DNA Polymerase Direction
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tRNA Function
tRNA Function
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rRNA function
rRNA function
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snRNA function
snRNA function
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Sigma factor
Sigma factor
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Transcription
Transcription
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RNA Polymerase I
RNA Polymerase I
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Translation
Translation
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Aminoacylation of tRNA
Aminoacylation of tRNA
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Ribosome
Ribosome
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Euchromatin
Euchromatin
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Heterochromatin
Heterochromatin
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Origin of Replication
Origin of Replication
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DNA Ligation
DNA Ligation
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S-phase
S-phase
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Transformation
Transformation
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Cistron
Cistron
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Promoter
Promoter
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Terminator
Terminator
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Nucleosome
Nucleosome
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Point Mutation
Point Mutation
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Sickle cell anemia
Sickle cell anemia
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What is tRNA?
What is tRNA?
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What are exons?
What are exons?
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Why are all three types of RNA needed?
Why are all three types of RNA needed?
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What role does each RNA play in translation?
What role does each RNA play in translation?
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What does RNA polymerase do?
What does RNA polymerase do?
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What is the role of the sigma factor?
What is the role of the sigma factor?
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What does RNA polymerase I do?
What does RNA polymerase I do?
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What is hnRNA?
What is hnRNA?
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Study Notes
- Nucleic acids are unique in their ability to direct their own replication. Offspring traits depend on precise DNA replication and transmission.
- DNA is the primary genetic material in most organisms, while RNA also serves as genetic material in some viruses.
- DNA is a polymer of deoxyribonucleotides, with a base pair defining a nucleotide pair.
- A nucleotide comprises a nitrogenous base, a pentose sugar (deoxyribose in DNA), and a phosphate group.
- Purines (Adenine and Guanine) and Pyrimidines (Cytosine, Uracil, and Thymine) are the nitrogenous bases.
- Cytosine is found in both DNA and RNA; Thymine is in DNA; Uracil is in RNA.
- A nucleoside is a nitrogenous base linked to a pentose sugar via an N-glycosidic bond.
- A nucleotide is a nucleoside with one or more phosphate groups linked to the 5'-OH of the nucleoside via phosphodiester linkages.
- Two nucleotides are linked by a 3'-5' phosphodiester bond, creating a polynucleotide chain.
- DNA is a double helix; two chains run in opposite directions (antiparallel).
- Bases are paired via hydrogen bonds; A-T (2 bonds) and G-C (3 bonds).
- The double helix's stability is enhanced by base-pairing interactions.
- DNA replication is semiconservative. Each daughter molecule has one original and one new strand.
- DNA replication requires enzyme DNA polymerase.
- DNA replication proceeds in one direction (5' to 3') during the process of continuous replication.
- DNA replication occurs at replication forks.
- Replication is initiated with the help of helicase enzyme.
- DNA polymerase is needed for DNA replication and it catalyzes the polymerization process from 5' to 3' on a template strand.
- Replication in one strand is continuous while in the other strand it's discontinuous forming Okazaki fragments, which are joined by DNA ligase.
- Replication of DNA takes place at the S phase of the cell cycle.
- Transcription is the process of copying genetic information from one strand of DNA into RNA, where only one segment of DNA is copied.
- RNA is made up of ribonucleotides containing RNA.
- mRNA acts as a template; tRNA carries amino acids and rRNA performs structural and catalytic functions.
- The mRNA serves as a template; tRNA transports amino acids and reads the genetic information; rRNA performs structural and catalytic functions during translation.
- The primary transcript is non-functional and contains both exons and introns, undergoing splicing to delete introns.
- In transcription, the promoter positioned at the 5' end recruits RNA polymerase.
- The terminator at the 3' end halts transcription, aided by rho factor.
- A cistron is a segment of DNA coding for a polypeptide.
- DNA is the primary genetic material in most organisms.
- The Central Dogma of molecular biology states that genetic information flows from DNA to RNA to protein.
- Proof that DNA is the genetic material was given by Hershey and Chase.
- Histones are rich in arginine and lysine and are positively charged proteins.
- A single nucleosome includes around 200 base pairs.
- Chromatin is the nucleosome's repeating unit.
- Heterochromatin is densely packed chromatin; euchromatin is loosely packed and transcriptionally active.
- DNA replicates semi-conservatively.
- The process of copying genetic information from DNA to RNA is called transcription.
- Genetic code is triplet, degenerate, and universal.
- A codon specifies an amino acid; three codons (stop codons) do not specify amino acids.
- Translation is the process of protein synthesis, transferring the genetic information from mRNA to a polypeptide.
- tRNA acts as an 'adapter' molecule to match mRNA codons to amino acids.
- rRNA plays structural and catalytic roles in protein synthesis.
- Griffith's experiment demonstrated bacterial transformation, showing that some substance from dead bacteria is transferred to live bacteria.
- Avery, MacLeod, and McCarty identified DNA as the transforming principle.
- Hershey and Chase proved that DNA is the genetic material of bacteriophages.
- Mutations alter genetic code, leading to changes in proteins and phenotypes.
- Some mutations, such as point mutations, are single base pair alterations.
- Insertion or deletion of multiple bases causes frame shift mutations.
- The Human Genome Project's aim was to map the entire human genetic sequence (genome).
- The genome contains 3.2 billion bases, with 20,000-25,000 genes.
- DNA fingerprinting (using SNPs) is employed for identifying individuals due to the differences in DNA sequences.
- The Central Dogma of molecular biology holds that genetic information flows from DNA to RNA to protein.
- Regulation of gene expression controls when and how genes are expressed.
- The lac operon controls the expression of genes involved in lactose metabolism.
- Genes are turned 'on' or 'off', thus regulated, when certain functions begin or cease.
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