DNA Fingerprinting and RFLP
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Questions and Answers

What is the purpose of labeling each set of primers with a different fluorescent label in multiplex PCR?

  • To amplify only one STR locus
  • To determine the length of the fragment
  • To distinguish each locus from the other (correct)
  • To run multiple PCR reactions in separate tubes

    • What is the advantage of capillary electrophoresis in multiplex STR analysis?

  • It can only be used for dot blots
  • It can only detect one fluorescently-labeled primer
  • It can only be used for Southern blotting
  • It can detect multiple fluorescently-labeled primers with non-overlapping primer sets (correct)

    • What is the result of an individual being heterozygous for each locus in a multiplex STR analysis?

  • Two different sized bands are seen for each locus (correct)
  • One band is seen for each locus
  • Three bands of different sizes are seen for each locus
  • Two bands of the same size are seen for each locus

    • What is the purpose of running the PCR products on an agarose gel in a multiplex STR analysis?

    <p>To separate the PCR products based on size</p> Signup and view all the answers

    What is the basis of the national database in countries like the United Kingdom and the United States?

    <p>Multiplex STR analysis</p> Signup and view all the answers

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