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Questions and Answers
What is the purpose of labeling each set of primers with a different fluorescent label in multiplex PCR?
What is the purpose of labeling each set of primers with a different fluorescent label in multiplex PCR?
- To amplify only one STR locus
- To determine the length of the fragment
- To distinguish each locus from the other (correct)
- To run multiple PCR reactions in separate tubes
What is the advantage of capillary electrophoresis in multiplex STR analysis?
What is the advantage of capillary electrophoresis in multiplex STR analysis?
- It can only be used for dot blots
- It can only detect one fluorescently-labeled primer
- It can only be used for Southern blotting
- It can detect multiple fluorescently-labeled primers with non-overlapping primer sets (correct)
What is the result of an individual being heterozygous for each locus in a multiplex STR analysis?
What is the result of an individual being heterozygous for each locus in a multiplex STR analysis?
- Two different sized bands are seen for each locus (correct)
- One band is seen for each locus
- Three bands of different sizes are seen for each locus
- Two bands of the same size are seen for each locus
What is the purpose of running the PCR products on an agarose gel in a multiplex STR analysis?
What is the purpose of running the PCR products on an agarose gel in a multiplex STR analysis?
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What is the basis of the national database in countries like the United Kingdom and the United States?
What is the basis of the national database in countries like the United Kingdom and the United States?
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