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DNA Fingerprinting and RFLP

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5 Questions

What is the purpose of labeling each set of primers with a different fluorescent label in multiplex PCR?

To distinguish each locus from the other

What is the advantage of capillary electrophoresis in multiplex STR analysis?

It can detect multiple fluorescently-labeled primers with non-overlapping primer sets

What is the result of an individual being heterozygous for each locus in a multiplex STR analysis?

Two different sized bands are seen for each locus

What is the purpose of running the PCR products on an agarose gel in a multiplex STR analysis?

To separate the PCR products based on size

What is the basis of the national database in countries like the United Kingdom and the United States?

Multiplex STR analysis

Learn about DNA fingerprinting, Restriction Fragment Length Polymorphisms (RFLPs), and autoradiographs. Understand the role of markers, positive control, and DNA probes in DNA fingerprinting.

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