Molecular Biology DNA Basics
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Questions and Answers

What type of DNA do prokaryotes have?

  • Circular DNAs (correct)
  • Single-stranded DNA
  • Linear DNAs
  • Double-stranded RNA

What is the main characteristic of euchromatin?

  • It consists of inactive genes.
  • It is tightly packed DNA.
  • It is less densely packed and consists of active genes. (correct)
  • It has limited gene expression.

Which base pairs with guanine in DNA?

  • Thymine
  • Adenine
  • Uracil
  • Cytosine (correct)

What does the term 'complementary bases' refer to?

<p>Bases in a nucleic acid structure that can hydrogen-bond to each other. (D)</p> Signup and view all the answers

The base composition of human DNA includes 30% adenine and 30% thymine. What are the percentages of guanine and cytosine?

<p>20% guanine and 20% cytosine (B)</p> Signup and view all the answers

What is the primary function of proteins synthesized during protein synthesis?

<p>To form various bodily structures and enzymes (A)</p> Signup and view all the answers

Which process involves the synthesis of mRNA from DNA?

<p>Transcription (D)</p> Signup and view all the answers

What is the main difference between eukaryotic and prokaryotic mRNA?

<p>Eukaryotic mRNA has a longer lifespan (A)</p> Signup and view all the answers

Which type of RNA primarily acts as a template for the synthesis of proteins?

<p>Messenger RNA (mRNA) (C)</p> Signup and view all the answers

What provides the sugar component in RNA molecules?

<p>Ribose (B)</p> Signup and view all the answers

What linkage connects nucleotides in RNA?

<p>Phosphodiester linkage (D)</p> Signup and view all the answers

Which of the following is considered a minor class of RNA?

<p>Small cytoplasmic RNA (scRNA) (C)</p> Signup and view all the answers

How is the structure of mRNA primarily characterized?

<p>Single-stranded linear molecule (B)</p> Signup and view all the answers

What primary function does messenger RNA (mRNA) serve in the process of protein synthesis?

<p>Carries genetic information from DNA for protein synthesis (C)</p> Signup and view all the answers

What structural feature distinguishes transfer RNA (tRNA) from other types of RNA?

<p>Cloverleaf shape (D)</p> Signup and view all the answers

Which of the following statements about the nucleotide sequence of mRNA is true?

<p>It is complementary to the template DNA strand (D)</p> Signup and view all the answers

How many tRNA molecules are typically associated with each amino acid during protein synthesis?

<p>At least one for each of the 20 amino acids (A)</p> Signup and view all the answers

What is the significance of the unusual bases found in tRNA?

<p>They play a role in binding tRNA to ribosomes (A)</p> Signup and view all the answers

What type of secondary structure can form within mRNA due to intrastrand base pairing?

<p>Hairpin loops (B)</p> Signup and view all the answers

In terms of structure, how does the tertiary form of tRNA appear?

<p>An inverted or tilted L shape (A)</p> Signup and view all the answers

What common characteristic do all types of RNA share?

<p>They are composed of nucleotides (B)</p> Signup and view all the answers

What is the primary role of the host cell in the production of new viruses?

<p>To replicate its own genome and proteins (B)</p> Signup and view all the answers

Which of the following describes recombinant DNA?

<p>DNA synthesized by splicing sequences from different organisms (C)</p> Signup and view all the answers

How are clones defined in the context of bacterial reproduction?

<p>Cells descended from a single cell with identical DNA (D)</p> Signup and view all the answers

Which technique is used to insert a 'foreign' gene into a host organism?

<p>Biochemical techniques in genetic engineering (A)</p> Signup and view all the answers

What are plasmids in the context of genetic engineering?

<p>Double-stranded DNA that can replicate independently (D)</p> Signup and view all the answers

What happens to transformed cells in bacterial cloning?

<p>They create multiple identical cells quickly (C)</p> Signup and view all the answers

What is the function of antibodies generated against inactive viruses or bacterial envelopes?

<p>To destroy any active viruses or bacteria (C)</p> Signup and view all the answers

What is one key advantage of using bacterial strains like E. coli in genetic engineering?

<p>Their rapid growth leading to large numbers of clones (B)</p> Signup and view all the answers

What is the purpose of using restriction enzymes in recombinant DNA technology?

<p>To cleave double-stranded DNA (D)</p> Signup and view all the answers

What is the role of DNA ligase in the recombinant DNA production process?

<p>To bind the gene of interest to a plasmid (D)</p> Signup and view all the answers

What does the polymerase chain reaction (PCR) primarily achieve?

<p>Amplification of DNA sequences (C)</p> Signup and view all the answers

During the process of recombinant DNA production, what is the first step after obtaining plasmid DNA?

<p>Cleavage of plasmid DNA (C)</p> Signup and view all the answers

Which of the following is NOT a requirement for carrying out PCR?

<p>Live bacterial culture (A)</p> Signup and view all the answers

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