Microbiology Sterilization Methods
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Questions and Answers

Combien de temps faut-il pour détruire les spores par autoclave ?

  • 3 secondes
  • 30 minutes
  • 10 minutes
  • 20 minutes (correct)

À quelle température les spores sont-elles détruites en 2 heures par chaleur sèche ?

  • -138 oC
  • -121 oC
  • -134 oC (correct)
  • -160 oC

Quelle est la température à laquelle seules les spores survivent après 10 minutes ?

  • -72 oC
  • -63 oC
  • -56 oC
  • -82 oC (correct)

Quelle est la température à laquelle les bactéries sont détruites en 17 secondes ?

<p>-72 oC (A)</p> Signup and view all the answers

Quel type de désinfectant est généralement requis en cas de déversements de milieux de culture ou de tampons?

<p>Désinfectant chimique (C)</p> Signup and view all the answers

Quel facteur peut influencer la résistance aux désinfectants des spores bactériennes?

<p>Structure de la paroi des bactéries à gram + et à gram - (C)</p> Signup and view all the answers

Qu'est-ce qui rend les virus nus plus résistants que les virus enveloppés?

<p>Destruction de la capside et libération de l’acide nucléique potentiellement infectieux (A)</p> Signup and view all the answers

Quelle substance est particulièrement résistante aux désinfectants et est responsable de la maladie de Creutzfeldt-Jakob?

<p>Prions (D)</p> Signup and view all the answers

Quel mécanisme d'action est le plus souvent associé aux moyens de décontamination chimique?

<p>Dénaturation des protéines (B)</p> Signup and view all the answers

Quel facteur influence la durée de contact nécessaire pour obtenir une réduction du nombre de germes de 99,999%?

<p>La concentration du désinfectant (A)</p> Signup and view all the answers

Quel moyen de décontamination est associé à l'utilisation d'un autoclave?

<p>Décontamination thermique (D)</p> Signup and view all the answers

Quel facteur influence la stabilité d'un produit désinfectant?

<p>Les instructions du fabricant (B)</p> Signup and view all the answers

Flashcards

Autoclave Spore Destruction Time

20 minutes is necessary to successfully destroy spores within an autoclave.

Spore Destruction by Dry Heat

A temperature of 134°C (273.2°F) is required to destroy spores via dry heat over a period of 2 hours.

Spore Survival at 82°C

Even after 10 minutes at 82°C (179.6°F), spores can still survive, demonstrating their resistance to heat.

Bacterial Destruction at 72°C

A temperature of 72°C (161.6°F) is sufficient to eliminate bacteria within a mere 17 seconds.

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Chemical Disinfectants for Spills

Chemical disinfectants are essential for cleaning up spills involving culture media or swabs, as they effectively kill harmful microbes.

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Bacterial Wall Structure and Disinfectant Resistance

The structural differences between Gram-positive and Gram-negative bacteria can influence their resistance to disinfectants.

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Naked Virus Resistance

Naked viruses, devoid of an outer envelope, are more resistant to disinfection because they lack an outer layer that can be easily disrupted.

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Prion Resistance to Disinfection

Prions, infectious proteins resistant to most disinfection methods, are responsible for Creutzfeldt-Jakob disease.

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Protein Denaturation in Chemical Decontamination

Denaturation of proteins is a common mechanism of action for chemical decontamination methods, disrupting their function and rendering them ineffective.

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Disinfectant Concentration and Contact Time

The concentration of the disinfectant directly influences the contact time needed to achieve a 99.999% reduction in microbial load, demonstrating its importance for effective disinfection.

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Thermal Decontamination using Autoclave

Thermal decontamination, using an autoclave, is a reliable method for eliminating microbes by applying heat and pressure.

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Disinfectant Stability and Manufacturer's Instructions

The stability of a disinfectant product is directly influenced by the manufacturer’s instructions, emphasizing the need to follow guidelines for optimal effectiveness.

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