Methods of Sterilization in Microbiology
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Questions and Answers

Which sterilization method involves slow protein denaturation through oxidation as its mode of action?

  • Moist heat
  • Dry heat (correct)
  • Filtration
  • Ethylene oxide

What is the mode of action of moist heat during sterilization?

  • Slow protein denaturation through oxidation
  • Protein and nucleic acid denaturation (correct)
  • Enhancement of bactericidal rate
  • Resistance to bactericidal agents

Why are bacterial endospores more resistant to heat compared to vegetative cells?

  • Due to higher water content
  • High susceptibility to bactericidal agents
  • Lack of SH groups in proteins
  • Contain calcium and dipicolinic acid (correct)

Which factor plays a significant role in affecting the efficiency of heat sterilization?

<p>Time and temperature (C)</p> Signup and view all the answers

Which of the following is NOT an official method for sterilization?

<p>UV Sterilization (D)</p> Signup and view all the answers

What is the main reason for the resistance of endospores to heat sterilization?

<p>Lack of calcium and dipicolinic acid (A)</p> Signup and view all the answers

What does heat in the presence of a bactericidal agent enhance during sterilization?

<p>Rate of reaction (C)</p> Signup and view all the answers

Why does moist heat cause death through protein and nucleic acid denaturation?

<p>Coagulation effect on proteins and nucleic acids (C)</p> Signup and view all the answers

What is the primary mode of action of sterilization by moist heat?

<p>Protein coagulation (A)</p> Signup and view all the answers

Which of the following is NOT an official method for sterilization?

<p>Pasteurization (B)</p> Signup and view all the answers

What is the primary reason for the resistance of endospores to heat during sterilization?

<p>Low water content and dipicolinic acid (B)</p> Signup and view all the answers

What is the key factor that affects the efficiency of heat sterilization?

<p>Time and temperature (D)</p> Signup and view all the answers

Which method of sterilization involves death due to bactericidal action with enhanced reaction rates?

<p>Dry heat (C)</p> Signup and view all the answers

What substance protects the SH groups of proteins during heat sterilization?

<p>Disulfide bonds (D)</p> Signup and view all the answers

What is the z value relevant to in sterilization?

<p>Thermal resistance (A)</p> Signup and view all the answers

Flashcards

Dry Heat Sterilization

Mode of action involving slow protein denaturation through oxidation.

Moist Heat Sterilization

Denaturation of proteins and nucleic acids through coagulation.

Endospore Resistance to Heat

Calcium and dipicolinic acid within endospores provide resistance to heat.

Factors Affecting Heat Sterilization

Time and temperature significantly impact the effectiveness of heat sterilization.

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UV Sterilization

UV sterilization is not an official method for sterilization.

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Endospore Resistance Mechanism

The presence of calcium and dipicolinic acid in endospores contributes to their heat resistance.

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Heat and Bactericidal Action

Heat enhances the rate of reaction during sterilization, promoting bactericidal action.

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Moist Heat Mechanism

Coagulation of proteins and nucleic acids causes death by disrupting their structure and function.

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Moist Heat's Primary Action

Protein coagulation is the primary mechanism of moist heat sterilization.

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Pasteurization

Pasteurization is not an officially recognized sterilization method.

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Endospore Heat Resistance

Low water content and dipicolinic acid contribute significantly to endospore resistance to heat during sterilization.

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Key Factor in Heat Sterilization

Time and temperature are critical factors determining the success of heat sterilization.

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Dry Heat Action

Dry heat sterilization leads to death due to bactericidal action with enhanced reaction rates.

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Disulfide Bond Protection

Disulfide bonds protect the SH groups of proteins during heat sterilization.

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Z Value in Sterilization

The z value represents the temperature increase required to reduce the decimal reduction time by a factor of 10.

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