Methods of Sterilization in Microbiology

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Which sterilization method involves slow protein denaturation through oxidation as its mode of action?

Moist heat

Dry heat (correct)

Filtration

Ethylene oxide

What is the mode of action of moist heat during sterilization?

Slow protein denaturation through oxidation

Protein and nucleic acid denaturation (correct)

Enhancement of bactericidal rate

Resistance to bactericidal agents

Why are bacterial endospores more resistant to heat compared to vegetative cells?

Due to higher water content

High susceptibility to bactericidal agents

Lack of SH groups in proteins

Contain calcium and dipicolinic acid (correct)

Which factor plays a significant role in affecting the efficiency of heat sterilization?

Time and temperature Signup and view all the answers

Which of the following is NOT an official method for sterilization?

UV Sterilization Signup and view all the answers

What is the main reason for the resistance of endospores to heat sterilization?

Lack of calcium and dipicolinic acid Signup and view all the answers

What does heat in the presence of a bactericidal agent enhance during sterilization?

Rate of reaction Signup and view all the answers

Why does moist heat cause death through protein and nucleic acid denaturation?

Coagulation effect on proteins and nucleic acids Signup and view all the answers

What is the primary mode of action of sterilization by moist heat?

Protein coagulation Signup and view all the answers

Which of the following is NOT an official method for sterilization?

Pasteurization Signup and view all the answers

What is the primary reason for the resistance of endospores to heat during sterilization?

Low water content and dipicolinic acid Signup and view all the answers

What is the key factor that affects the efficiency of heat sterilization?

Time and temperature Signup and view all the answers

Methods of Sterilization in Microbiology

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Questions and Answers

Which sterilization method involves slow protein denaturation through oxidation as its mode of action?

What is the mode of action of moist heat during sterilization?

Why are bacterial endospores more resistant to heat compared to vegetative cells?

Which factor plays a significant role in affecting the efficiency of heat sterilization?

Which of the following is NOT an official method for sterilization?

What is the main reason for the resistance of endospores to heat sterilization?

What does heat in the presence of a bactericidal agent enhance during sterilization?

Why does moist heat cause death through protein and nucleic acid denaturation?

What is the primary mode of action of sterilization by moist heat?

Which of the following is NOT an official method for sterilization?

What is the primary reason for the resistance of endospores to heat during sterilization?

What is the key factor that affects the efficiency of heat sterilization?

Which method of sterilization involves death due to bactericidal action with enhanced reaction rates?

What substance protects the SH groups of proteins during heat sterilization?

What is the z value relevant to in sterilization?

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