Microbiology: Sterilization and Microbial Control
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Questions and Answers

What is a common method of sterilization besides autoclaving?

  • Lyophilization
  • Filtration (correct)
  • Ionizing radiation
  • Pasteurization
  • What is a mechanism of action of microbial control agents that involves rupturing the cell membrane?

  • Damage to proteins
  • Inhibition of cell wall formation
  • Denaturation of nucleic acids
  • Alteration of cell membrane permeability (correct)
  • What is a type of chemical method of sterilization?

  • Radiation
  • Fumigation
  • Ethylene oxide (correct)
  • Incineration
  • What is a mechanism of action of microbial control agents that involves damaging cell wall components?

    <p>Damage to the cell wall</p> Signup and view all the answers

    What is an example of a dry heat sterilization method?

    <p>Incineration</p> Signup and view all the answers

    Which microbial control agent damages proteins and nucleic acids?

    <p>Glutaraldehyde</p> Signup and view all the answers

    What is the primary mechanism of action of autoclaving?

    <p>Damage to cell wall</p> Signup and view all the answers

    Which method of sterilization is most effective against heat-resistant microorganisms?

    <p>Ethylene oxide</p> Signup and view all the answers

    What is the primary difference between autoclaving and dry heat sterilization?

    <p>Moisture levels</p> Signup and view all the answers

    Which of the following mechanisms of action is NOT a common method of microbial control?

    <p>Dehydration of microorganisms</p> Signup and view all the answers

    Study Notes

    Sterilization Procedures

    • Autoclaving: a method that uses high-pressure steam to kill microorganisms
    • Dry heat sterilization: a method that uses hot air to kill microorganisms
    • Filtration: a method that uses a physical barrier to remove microorganisms from a solution
    • Chemical methods:
      • Glutaraldehyde: a chemical disinfectant that kills microorganisms
      • Ethylene oxide: a chemical sterilant that is used to sterilize heat-sensitive materials

    Mechanisms of Action of Microbial Control Agents

    • Damage to the cell wall: microorganisms are killed by disrupting the structure and function of their cell walls
    • Alteration of cell membrane permeability: microorganisms are killed by changing the permeability of their cell membranes, preventing them from functioning properly
    • Damage to proteins and nucleic acids: microorganisms are killed by damaging their proteins and nucleic acids, preventing them from carrying out essential cellular functions

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    Description

    Test your knowledge of sterilization procedures, including autoclaving, dry heat, and chemical methods, as well as the mechanisms of action of microbial control agents. Learn about the effects on cell walls, membranes, proteins, and nucleic acids.

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