Microbiology: Sterilization and Microbial Control

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Questions and Answers

What is a common method of sterilization besides autoclaving?

  • Lyophilization
  • Filtration (correct)
  • Ionizing radiation
  • Pasteurization

What is a mechanism of action of microbial control agents that involves rupturing the cell membrane?

  • Damage to proteins
  • Inhibition of cell wall formation
  • Denaturation of nucleic acids
  • Alteration of cell membrane permeability (correct)

What is a type of chemical method of sterilization?

  • Radiation
  • Fumigation
  • Ethylene oxide (correct)
  • Incineration

What is a mechanism of action of microbial control agents that involves damaging cell wall components?

<p>Damage to the cell wall (C)</p>
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What is an example of a dry heat sterilization method?

<p>Incineration (B)</p>
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Which microbial control agent damages proteins and nucleic acids?

<p>Glutaraldehyde (D)</p>
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What is the primary mechanism of action of autoclaving?

<p>Damage to cell wall (D)</p>
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Which method of sterilization is most effective against heat-resistant microorganisms?

<p>Ethylene oxide (D)</p>
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What is the primary difference between autoclaving and dry heat sterilization?

<p>Moisture levels (D)</p>
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Which of the following mechanisms of action is NOT a common method of microbial control?

<p>Dehydration of microorganisms (C)</p>
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Flashcards

Filtration sterilization

A method of sterilization that involves passing a liquid or gas through a filter with pores small enough to trap microorganisms.

Alteration of cell membrane permeability

This mechanism targets the cell membrane, causing damage and ultimately disrupting the microbe's ability to function.

Ethylene oxide sterilization

A method of sterilization that involves using a chemical agent like ethylene oxide gas to kill microbes.

Damage to the cell wall

This mechanism directly targets the cell wall, leading to weakening and ultimately cell lysis.

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Incineration

A dry heat sterilization method that involves exposing materials to extremely high temperatures, burning and destroying microorganisms.

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Glutaraldehyde

A strong sterilizing agent that works by denaturing proteins and nucleic acids within microorganisms.

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Autoclaving

The primary method of action in autoclaving, where the high pressure and temperature damage the cell wall.

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Ethylene oxide sterilization

This sterilization method is highly effective against heat-resistant microbes due to the penetrating nature of the chemical agent.

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Autoclaving vs. dry heat sterilization

The primary difference between these methods is the presence or absence of moisture during the sterilization process.

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Common mechanisms of microbial control

The primary mechanisms of action of microbial control agents focus on damaging the cell wall, altering membrane permeability, or denaturing proteins and nucleic acids.

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Study Notes

Sterilization Procedures

  • Autoclaving: a method that uses high-pressure steam to kill microorganisms
  • Dry heat sterilization: a method that uses hot air to kill microorganisms
  • Filtration: a method that uses a physical barrier to remove microorganisms from a solution
  • Chemical methods:
    • Glutaraldehyde: a chemical disinfectant that kills microorganisms
    • Ethylene oxide: a chemical sterilant that is used to sterilize heat-sensitive materials

Mechanisms of Action of Microbial Control Agents

  • Damage to the cell wall: microorganisms are killed by disrupting the structure and function of their cell walls
  • Alteration of cell membrane permeability: microorganisms are killed by changing the permeability of their cell membranes, preventing them from functioning properly
  • Damage to proteins and nucleic acids: microorganisms are killed by damaging their proteins and nucleic acids, preventing them from carrying out essential cellular functions

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