Microbiology Quiz on Spirochetes and Chlamydia
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Questions and Answers

Which disease is specifically associated with Borrelia burgdorferi?

  • Psittacosis
  • Inclusion conjunctivitis
  • Syphilis
  • Lyme disease (correct)
  • Which of the following correctly describes Chlamydia trachoma?

  • Can cause genital infections (correct)
  • Is a Gram-positive bacterium
  • Is exclusively free-living
  • Causes respiratory infections
  • What characteristic differentiates Rickettsiae from Chlamydia?

  • Rickettsiae are primarily respiratory pathogens
  • Chlamydia can survive outside a host cell
  • Chlamydia is exclusively a vector-borne disease
  • Rickettsiae are Gram-negative intracellular pathogens (correct)
  • Which structural feature is unique to spirochetes like Treponema pallidum?

    <p>Outer sheath with glycosaminoglycan coating (C)</p> Signup and view all the answers

    How do endoflagella contribute to the motility of spirochetes?

    <p>By flexing and rotating around the protoplasmic cylinder (D)</p> Signup and view all the answers

    What is a common complication of B.miyamotoi infection in severely immunocompromised patients?

    <p>Meningoencephalitis (C)</p> Signup and view all the answers

    Which treatment is recommended for patients with neurologic involvement due to B.miyamotoi infection?

    <p>Intravenous ceftriaxone (D)</p> Signup and view all the answers

    What is the recommended approach for diagnosing B.miyamotoi infection?

    <p>Enzyme immunoassay followed by Western blot (D)</p> Signup and view all the answers

    In which months is the nymphal stage of Ixodes ticks most active?

    <p>May to July (D)</p> Signup and view all the answers

    Which of the following is a characteristic of Rickettsia?

    <p>Obligate intracellular parasites (A)</p> Signup and view all the answers

    What disease is caused by Epidemic typhus, which is related to Rickettsia?

    <p>Rocky Mountain spotted fever (A)</p> Signup and view all the answers

    What is a commonly used preventive measure after a tick bite in highly endemic regions?

    <p>Prophylactic doxycycline (A)</p> Signup and view all the answers

    Which of the following describes the life cycle of Rickettsia?

    <p>Requires live cells for cultivation (D)</p> Signup and view all the answers

    Which subspecies of Treponema is responsible for causing Pinta?

    <p>Treponema carateum (A)</p> Signup and view all the answers

    What is the primary method of transmission for leptospirosis?

    <p>Contact with contaminated urine (C)</p> Signup and view all the answers

    Which of the following is NOT a symptom of leptospirosis?

    <p>Skin depigmentation (C)</p> Signup and view all the answers

    Which vector is primarily responsible for the transmission of Borrelia hermsii?

    <p>Ticks (D)</p> Signup and view all the answers

    What characterizes the staining properties of Borrelia species?

    <p>They stain readily with bacteriologic dyes (D)</p> Signup and view all the answers

    What is a distinctive feature of the antibodies produced after Borrelia infection?

    <p>They change over the course of a single infection (A)</p> Signup and view all the answers

    Which of the following best describes the structure of Leptospira?

    <p>Tightly regular individual coils with bends (C)</p> Signup and view all the answers

    What is the common name for the disease caused by Borrelia burgdorferi?

    <p>Lyme disease (A)</p> Signup and view all the answers

    How is the treatment typically managed for patients with penicillin hypersensitivity?

    <p>Doxycycline is used (D)</p> Signup and view all the answers

    What is the primary environmental source for leptospirosis outbreaks?

    <p>Wildlife urine (D)</p> Signup and view all the answers

    What is the primary function of the major outer membrane protein (MOMP) in Chlamydiaceae?

    <p>Acts as an immunogenic factor (A)</p> Signup and view all the answers

    Which of the following diseases is NOT associated with Bartonella species?

    <p>Pneumonia (B)</p> Signup and view all the answers

    Which treatment is specifically indicated for cat-scratch disease?

    <p>Erythromycin (A), Azithromycin (D)</p> Signup and view all the answers

    In the development cycle of Chlamydia, what is the role of the reticulate body (RB)?

    <p>Grows within host cell vacuoles (C), Converts into elementary bodies (D)</p> Signup and view all the answers

    What distinguishes the elementary body (EB) in the life cycle of Chlamydia from the reticulate body (RB)?

    <p>EB is the infectious form, while RB is not (B)</p> Signup and view all the answers

    Which pathogen requires metabolites from host cells for survival?

    <p>Chlamydia trachomatis (B)</p> Signup and view all the answers

    What is the mechanism by which Chlamydia establishes infection in host cells?

    <p>Induction of its own endocytosis (D)</p> Signup and view all the answers

    What is a characteristic of Bartonella species in terms of culturing?

    <p>Fastidious, requiring specific culture conditions (A)</p> Signup and view all the answers

    How does Chlamydia prevent host cell destruction during its growth phase?

    <p>By inhibiting lysosomal fusion (B)</p> Signup and view all the answers

    Which of the following statements about Coxiella burnetii is true?

    <p>High-temperature short-time pasteurization can destroy it (D)</p> Signup and view all the answers

    What is the primary reservoir for Rickettsia pox?

    <p>House mouse and semidomestic rodents (A)</p> Signup and view all the answers

    Which statement is true about endemic louse-borne typhus fever?

    <p>Diagnosis is largely based on serology tests. (A)</p> Signup and view all the answers

    What distinguishes Rickettsia typhi from Rickettsia prowazekii in terms of symptom severity?

    <p>Rickettsia prowazekii is more severe. (B)</p> Signup and view all the answers

    What is the mode of transmission for Q fever caused by Coxiella burnetti?

    <p>Inhalation of spores or aerosols (A)</p> Signup and view all the answers

    Which of the following is true regarding the treatment of endemic typhus?

    <p>Doxycycline should be initiated immediately on suspicion. (C)</p> Signup and view all the answers

    What characteristic feature is associated with Rickettsia pox at the site of the mite bite?

    <p>Eschar formation (D)</p> Signup and view all the answers

    Coxiella burnetti is resistant to what environmental condition?

    <p>Drying (C)</p> Signup and view all the answers

    Which of the following correctly describes the epidemiological conditions favoring the spread of endemic louse-borne typhus fever?

    <p>Disaster situations leading to overcrowding. (D)</p> Signup and view all the answers

    What is a unique characteristic of Coxiella burnetti compared to other rickettsiae?

    <p>It produces resistant spore-like structures. (B)</p> Signup and view all the answers

    In untreated cases of endemic louse-borne typhus fever, which age group experiences the highest fatality rate?

    <p>Elderly individuals over 60 (D)</p> Signup and view all the answers

    Flashcards

    B. miyamotoi infection

    A bacterial infection that can cause meningoencephalitis, especially in people with weakened immune systems.

    Meningoencephalitis

    A common symptom of B. miyamotoi infection, particularly in immunocompromised patients.

    Bacteremia

    The presence of bacteria in the bloodstream, which can lead to widespread infection.

    Rickettsiaceae

    A family of bacteria that includes Rickettsia, a genus responsible for various diseases.

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    Rickettsioses

    A group of diseases caused by Rickettsia bacteria.

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    Rickettsia

    A bacteria belonging to the Rickettsiaceae family, known for causing various diseases.

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    Vasculitis

    Inflammation of blood vessels, often caused by Rickettsia infections.

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    Epidemic typhus

    A serious form of typhus caused by Rickettsia prowazekii, often spread by lice.

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    Treponema pallidum subspecies pertenue

    A subspecies of Treponema pallidum that causes yaws, a tropical disease characterized by skin lesions that start as a primary ulcer and spread to other parts of the body.

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    Yaws

    A tropical infection caused by Treponema pallidum subspecies pertenue.

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    Pinta

    A superficial skin infection caused by Treponema carateum, characterized by depigmentation and scarring of the skin.

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    Trench Fever

    A bacterial infection caused by Bartonella quintana, typically spread through the bite of an infected louse.

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    Leptospira

    A genus of bacteria that includes the species Leptospira interrogans, which causes leptospirosis.

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    Cat-scratch Disease

    A bacterial infection caused by Bartonella henselae, commonly transmitted through a cat's scratch or bite.

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    Leptospirosis

    A zoonotic disease caused by Leptospira interrogans, transmitted through contact with contaminated animal urine.

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    Bacillary Angiomatosis

    A type of bacterial infection that affects blood vessels, particularly in individuals with compromised immune systems.

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    Borrelia

    A genus of bacteria that includes Borrelia burgdorferi, the causative agent of Lyme disease

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    Chlamydiaceae

    A group of bacteria that are obligate intracellular parasites, meaning they require a host cell to survive and reproduce.

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    Lyme disease

    A tick-borne disease caused by Borrelia burgdorferi, characterized by a distinctive rash, fever, and potential complications affecting the joints, heart, and nervous system.

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    Elementary Body (EB)

    The metabolically inactive form of Chlamydia that is released from infected host cells and is capable of infecting new cells.

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    Reticulate Body (RB)

    The actively dividing and non-infectious form of Chlamydia that grows inside host cell vacuoles.

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    Borrelia hermsii

    A type of Borrelia causing a relapsing fever that is transmitted by ticks.

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    Major Outer Membrane Protein (MOMP)

    A protein found on the outer membrane of Chlamydia, which is important for the immune response to the bacteria.

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    Borrelia miyamotoi

    A gram-negative spirochete, which means it has a spiral shape and does not stain purple with Gram staining.

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    Relapsing fever

    A type of fever caused by Borrelia hermsii, characterized by recurrent high fevers and chills.

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    Lysosomal Fusion Inhibition

    A process where Chlamydia inhibits the fusion of lysosomes with the vacuole where it resides, protecting itself from destruction.

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    Chlamydia Protease-like Activity Factor (CPAF)

    A chlamydia-related protein that controls the timing of cell death, delaying it during the growth phase but triggering it at the release stage.

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    Inclusion

    A membrane-bound vesicle created by Chlamydia within the host cell, serving as a protected environment for its replication and growth.

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    Spirochetes

    Spiral-shaped bacteria that are elongated, motile (able to move), and flexible. They have characteristic endoflagella, which are flagella-like structures that wrap around the cell and help with movement.

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    Borrelia burgdorferi

    The causative agent of Lyme disease, a tick-borne illness characterized by a distinctive bull's-eye rash, fever, and potential complications affecting the joints, heart, and nervous system.

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    Treponema pallidum

    The causative agent of syphilis, a sexually transmitted infection that progresses through stages and can affect various organs.

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    Chlamydia

    A genus of bacteria that includes Chlamydia trachomatis, the causative agent of several infections, including trachoma, genital infections, and inclusion conjunctivitis.

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    Rickettsia pox

    A rickettsial disease caused by Rickettsia akari, transmitted through rodent mites. Characterized by a black scab at the bite site and a skin rash that doesn't affect palms and soles.

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    Rickettsia akari

    An infectious agent causing Rickettsia pox, transmitted through rodent mites such as those on house mice and rodents.

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    Endemic Louse-Borne Typhus Fever

    A type of rickettsial disease caused by Rickettsia prowazekii, transmitted by body lice.

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    Rickettsia prowazekii

    The causative agent for Endemic Louse-Borne Typhus Fever, a rickettsial disease transmitted through body lice.

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    Endemic (Murine) Typhus

    A rickettsial disease caused by Rickettsia typhi, transmitted by rat fleas and found mainly in urban areas.

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    Rickettsia typhi

    The causative agent for Endemic (Murine) Typhus, a rickettsial disease transmitted through rat fleas.

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    Coxiella burnetti

    An intracellular parasite that causes Q fever. It is known for its resistance to heat and drying due to its spore-like structure. It can survive in various animal products, including milk and feces.

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    Q fever

    A bacterial infection caused by Coxiella burnetti, usually contracted through inhalation of contaminated materials. It causes pneumonia, fever, and sometimes hepatitis.

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    Study Notes

    Lecture 12: Microbiology - Spirochetes, Rickettsiae, Chlamydia

    • This lecture covers the biological characteristics, infection, pathogenesis, immunity, diagnosis, treatment, and prevention of microbial infections caused by spirochetes, rickettsiae, and chlamydia.

    Spirochetes

    • Gram-negative human pathogens

    • Free-living saprophytes (or commensals) of animals; not considered primary pathogens

    • Genera include Treponema, Leptospira, Borrelia

    • T. pallidum has an outer sheath/glyco-aminoglycan coating. The outer membrane within this coating contains peptidoglycan to maintain structural integrity.

    • Spirochetes are elongated, motile, and flexible bacteria; they are spiral-shaped along their long axis.

    • Spirochetes contain endoflagella, which are polar flagella wound along the axial filament. They're situated between the outer membrane and cell wall.

    • Motility involves rotation and flexion of the spiral structure.

    • Many spirochetes stain poorly with Gram-stains; visualization may involve special staining techniques like dark-field microscopy or immunofluorescence.

    • T. pallidum, the causative agent of syphilis, is highly fastidious and sensitive, unable to survive long outside the host. It's sexually transmitted and transplacental.

    • Live cells exhibit characteristic rotating motility and sudden 90-degree angle flexion.

    • T. pallidum is susceptible to drying and killed by various detergents/disinfectants.

    • Spirochetes are visualized via immunofluorescent stains or dark-field illumination.

    • Some are not readily seen under a light microscope.

    • Genus Treponema: thin, regular, coiled cells; may be aerobic or anaerobic; live in oral cavity, intestinal tract, perigenital regions of humans and animals. Many are part of nasopharyngeal flora. Pathogens are strict parasites with complex growth requirements requiring live cells for cultivation.

    • Treponema pallidum: humans are the natural host. The spirochete is the causative agent for syphilis.

    • Spirochaetales Associated Human Diseases: A table listing genera, species, and their associated diseases; includes Syphilis, Bejel, Yaws, Pinta, Lyme disease, Epidemic relapsing fever, Endemic relapsing fever, Leptospirosis.

    • Spirochetal diseases include Trench mouth; (Vincent infection) caused by overgrowth of spirochetae. The infection correlates with immunocompromise, severe malnutrition, and neglect of basic hygiene. This historically happened to soldiers in trenches during World War I.

    • Treponema pallidum: the causative agent of syphilis. The great pox was recognized in the 16th century and rapidly spread in Europe.

    • Syphilis: The current name for the disease. In its explanation comes from a 18th-century poem and was written by Girolamo Fracastoro.

    • Syphilis pathogenesis and host response: T. pallidum is an exclusively human pathogen; binds to epithelium, and multiplies; penetrates capillaries. Infection rapidly moves into circulation and is established in distant tissues within hours. Untreated syphilis manifests in three clinical stages (primary, secondary, tertiary). Infection is contracted through direct sexual contact or trans-placental route. Tertiary syphilis lacks infectivity.

    Rickettsiae

    • Small, Gram-negative, non-motile, pleomorphic bacteria.

    • Obligate intracellular parasites that are involved in the life cycle of ticks, fleas, and lice.

    • Bacteria invade endothelial cells causing necrosis in the vascular linings and lead to vasculitis, vascular leakage, and thrombosis.

    • Causes rickettsioses, important emerging diseases.

    • Specific Rickettsioses: Includes diseases like epidemic typhus, endemic typhus, Rocky Mountain spotted fever, scrub typhus, and others.

    • Rocky Mountain Spotted Fever (RMSF) is commonly found in the Southeast and eastern seaboard of the U.S.

    • RMSF: Symptoms (rash, fever, myalgia), transmission by ticks, and complications.

    • Rickettsia pox: Caused by Rickettsia akari transmitted by rodent mites; distinguishing feature is an eschar at the bite site and vascular rash. House mice are the primary reservoirs.

    • Endemic Louse-Borne Typhus Fever: Caused by R. prowazekii, transmitted by body lice; appears in times of war or famine; highly infectious.

    • Endemic (Murine) Typhus: Caused by R. typhi, transmitted by rat fleas; resembles typhus, but less severe.

    Coxiella Burnetti

    • Causes Q fever; an intracellular parasite that produces a resistant spore. Q fever is harbored in a wide assortment of vertebrates and arthropods. Infectious material includes urine, feces, milk, and airborne particles.
    • Q fever often presents as pneumonia, fever, and hepatitis.

    Bartonella Species

    • Bartonella quintana causes Trench Fever.
    • Bartonella henselae causes cat-scratch disease, a lymphatic infection from a claw injury by cats.
    • Both are small gram-negative bacteria cultured on blood agar.

    Chlamydiaceae

    • Obligate intracellular parasites that require metabolites from host cells and are small gram-negative. They have a lipopolysaccharide- containing outer membrane and lack peptidoglycan.

    • Chlamydia trachomatis can infect the mucous membranes of the eyes, genitourinary tract, and lungs, causing conditions like trachoma, inclusion conjunctivitis, and more.

    • Chlamydophila pneumoniae and psittaci cause diseases like pneumonia and psittacosis (flu-like viral infection) in humans.

    • Chlamydia alternates between two stages: elementary bodies (EB) are small, metabolically inactive, infectious forms, whereas reticulate bodies (RB) are non-infectious, actively dividing forms growing within host cell vacuoles.

    • Chlamydia trachomatis: Human reservoir, different strains cause various diseases. Infections includes trachoma (ocular infection, possible blindness), inclusion conjunctivitis (in newborns), and sex-transmitted diseases.

    • Chlamydophila pneumoniae: Often associated with outbreaks of community-acquired pneumonia. Also causing pharyngitis and laryngitis.

    • C. psittaci: Causes ornithosis or psittacosis (birds)

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    Test your knowledge on key characteristics and diseases associated with spirochetes like Treponema pallidum and Chlamydia trachomatis. This quiz will cover important differentiators and structural features relevant to these microorganisms. Ideal for students studying microbiology or infectious diseases.

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