18 Questions
What is the main reason for centrifuging CSF at high speed for 10 minutes?
To concentrate cells in a small volume for counting
What is the characteristic of a normal adult's CSF differential count?
70% lymphocytes and 30% monocytes
What is the purpose of using a Romanowsky stain in CSF examination?
To distinguish between different types of WBCs
What is the advantage of using a cytocentrifuge in CSF examination?
It improves the cell yield and preserves the cell morphology
Which of the following microbiological tests is used to detect bacterial and cryptococcal antigens?
Latex agglutination tests
What is the reason for performing a Gram's smear on CSF?
To diagnose bacterial meningitis
What is the primary purpose of centrifuging a 50 mL fluid specimen in a laboratory setting?
To remove the supernatant and isolate the sediment
What type of stain is typically used for air-dried smears?
May-Grünwald-Giemsa (MGG)
What is the advantage of preparing a cell block from a residual sample?
It enables the performance of special stains and immunocytochemistry
What is the primary component of a benign effusion fluid?
A mixture of different types of cells
What is the purpose of treating the residual sediment with plasma and thrombin in cell block preparation?
To form a clot
What is the primary use of polymerase chain reaction in this laboratory setting?
To identify viruses
What is the term for an increase in cell count in CSF?
Pleocytosis
In which condition is thick viscous CSF typically seen?
All of the above
What is the purpose of microscopic examination of all CSF samples?
To identify white blood cells even in clear-appearing CSF
What is the significance of dilution factor in cell count calculation?
It is used to multiply the number of cells counted
What is a common cause of increased cell count in CSF?
Injection of radiographic contrast media
How is the total leukocyte count calculated in an undiluted CSF sample?
By counting the number of cells in 5 squares
Test your knowledge of various laboratory techniques used in microbiology, including serologic tests, culture, PCR, and processing of fluid specimens. Learn about the importance of centrifugation and smear preparation for staining and analysis.
Make Your Own Quizzes and Flashcards
Convert your notes into interactive study material.
Get started for free
