Microbiology Lab Techniques

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What is the main reason for centrifuging CSF at high speed for 10 minutes?

To concentrate cells in a small volume for counting (correct)

To separate the different types of WBCs

To increase the cell count

To preserve the cell morphology

What is the characteristic of a normal adult's CSF differential count?

50% lymphocytes and 50% monocytes

70% lymphocytes and 30% monocytes (correct)

40% lymphocytes and 60% monocytes

30% lymphocytes and 70% monocytes

What is the purpose of using a Romanowsky stain in CSF examination?

To identify bacteria and fungi

To determine the protein content of CSF

To distinguish between different types of WBCs (correct)

To detect the presence of blood in CSF

What is the advantage of using a cytocentrifuge in CSF examination?

It improves the cell yield and preserves the cell morphology (D) Signup and view all the answers

Which of the following microbiological tests is used to detect bacterial and cryptococcal antigens?

Latex agglutination tests (C) Signup and view all the answers

What is the reason for performing a Gram's smear on CSF?

To diagnose bacterial meningitis (B) Signup and view all the answers

What is the primary purpose of centrifuging a 50 mL fluid specimen in a laboratory setting?

To remove the supernatant and isolate the sediment (D) Signup and view all the answers

What type of stain is typically used for air-dried smears?

May-Grünwald-Giemsa (MGG) (C) Signup and view all the answers

What is the advantage of preparing a cell block from a residual sample?

It enables the performance of special stains and immunocytochemistry (A) Signup and view all the answers

What is the primary component of a benign effusion fluid?

A mixture of different types of cells (C) Signup and view all the answers

What is the purpose of treating the residual sediment with plasma and thrombin in cell block preparation?

To form a clot (D) Signup and view all the answers

What is the primary use of polymerase chain reaction in this laboratory setting?

To identify viruses (A) Signup and view all the answers

What is the term for an increase in cell count in CSF?

Pleocytosis (C) Signup and view all the answers

In which condition is thick viscous CSF typically seen?

All of the above (D) Signup and view all the answers

What is the purpose of microscopic examination of all CSF samples?

To identify white blood cells even in clear-appearing CSF (C) Signup and view all the answers

What is the significance of dilution factor in cell count calculation?

It is used to multiply the number of cells counted (C) Signup and view all the answers

What is a common cause of increased cell count in CSF?

Injection of radiographic contrast media (C) Signup and view all the answers

How is the total leukocyte count calculated in an undiluted CSF sample?

By counting the number of cells in 5 squares (D) Signup and view all the answers