Microbiology Lab Techniques

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Questions and Answers

What is the main reason for centrifuging CSF at high speed for 10 minutes?

  • To concentrate cells in a small volume for counting (correct)
  • To separate the different types of WBCs
  • To increase the cell count
  • To preserve the cell morphology

What is the characteristic of a normal adult's CSF differential count?

  • 50% lymphocytes and 50% monocytes
  • 70% lymphocytes and 30% monocytes (correct)
  • 40% lymphocytes and 60% monocytes
  • 30% lymphocytes and 70% monocytes

What is the purpose of using a Romanowsky stain in CSF examination?

  • To identify bacteria and fungi
  • To determine the protein content of CSF
  • To distinguish between different types of WBCs (correct)
  • To detect the presence of blood in CSF

What is the advantage of using a cytocentrifuge in CSF examination?

<p>It improves the cell yield and preserves the cell morphology (D)</p> Signup and view all the answers

Which of the following microbiological tests is used to detect bacterial and cryptococcal antigens?

<p>Latex agglutination tests (C)</p> Signup and view all the answers

What is the reason for performing a Gram's smear on CSF?

<p>To diagnose bacterial meningitis (B)</p> Signup and view all the answers

What is the primary purpose of centrifuging a 50 mL fluid specimen in a laboratory setting?

<p>To remove the supernatant and isolate the sediment (D)</p> Signup and view all the answers

What type of stain is typically used for air-dried smears?

<p>May-Grünwald-Giemsa (MGG) (C)</p> Signup and view all the answers

What is the advantage of preparing a cell block from a residual sample?

<p>It enables the performance of special stains and immunocytochemistry (A)</p> Signup and view all the answers

What is the primary component of a benign effusion fluid?

<p>A mixture of different types of cells (C)</p> Signup and view all the answers

What is the purpose of treating the residual sediment with plasma and thrombin in cell block preparation?

<p>To form a clot (D)</p> Signup and view all the answers

What is the primary use of polymerase chain reaction in this laboratory setting?

<p>To identify viruses (A)</p> Signup and view all the answers

What is the term for an increase in cell count in CSF?

<p>Pleocytosis (C)</p> Signup and view all the answers

In which condition is thick viscous CSF typically seen?

<p>All of the above (D)</p> Signup and view all the answers

What is the purpose of microscopic examination of all CSF samples?

<p>To identify white blood cells even in clear-appearing CSF (C)</p> Signup and view all the answers

What is the significance of dilution factor in cell count calculation?

<p>It is used to multiply the number of cells counted (C)</p> Signup and view all the answers

What is a common cause of increased cell count in CSF?

<p>Injection of radiographic contrast media (C)</p> Signup and view all the answers

How is the total leukocyte count calculated in an undiluted CSF sample?

<p>By counting the number of cells in 5 squares (D)</p> Signup and view all the answers

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