Clinical Parasitology Lab: Kato Katz Technique

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Questions and Answers

What is the purpose of the mesh screen in the Kato Katz technique?

To remove large particles from the fecal sample (correct)

To remove small particles from the fecal sample

To mix the feces with glycerol

To apply pressure to the fecal sample

What is the multiplication factor used if a 41.7 mg template is used?

24 (correct)

What is the purpose of soaking the cellophane strips?

To increase the visibility of the eggs

To clear the fecal material around the eggs (correct)

To preserve the eggs in the fecal sample

To remove impurities from the fecal sample

What is the unit of measurement used to report the results of the Kato Katz technique?

Eggs per gram (EPG) Signup and view all the answers

What is the minimum time required to soak the cellophane strips?

24 hours Signup and view all the answers

Which of the following worms can be recognized in the Kato Katz preparation for many months?

Ascaris and Trichuris Signup and view all the answers

What is the purpose of the template in the Kato Katz technique?

To hold the fecal sample in place Signup and view all the answers

What is the next step after transferring a small amount of feces onto the newspaper?

Press the screen on top of the fecal sample Signup and view all the answers

What is the purpose of the applicator sticks?

To transfer a small amount of feces onto the newspaper Signup and view all the answers

How many eggs per gram of stool would you report if you found 350 eggs in the Kato Katz technique?

8,400 EPG Signup and view all the answers

What is the primary purpose of the Kato Katz technique?

To diagnose and quantify intestinal helminth infections Signup and view all the answers

Which of the following helminths is not detected using the Kato Katz technique?

Giardia lamblia Signup and view all the answers

What is the recommended duration for clearing the fecal material prior to examination under the microscope?

1 hour Signup and view all the answers

What is the advantage of using the Kato Katz technique in areas with moderate to high transmission rates of STH?

It is recommended by the WHO Signup and view all the answers

What is the limitation of the Kato Katz technique?

It has low sensitivity, particularly for light-intensity helminth infections Signup and view all the answers

What is the purpose of inverting the microscope slide and pressing the fecal sample against the cellophane?

To spread the sample evenly Signup and view all the answers

What should be done if an excess of glycerol is present on the upper surface of the cellophane?

Wipe off the excess with a small piece of toilet paper or absorbent tissue Signup and view all the answers

What is the correct way to handle the slide after pressing the fecal sample against the cellophane?

Gently slide the microscope slide sideways Signup and view all the answers

What is the purpose of the Kato Katz technique in post-treatment surveys?

To measure the impact of control by measuring changes in the prevalence and intensity of infection Signup and view all the answers

What is the material used to cover the fecal sample on the slide?

A glycerol-soaked cellophane strip Signup and view all the answers

Study Notes

Kato Katz Technique

Used for qualitative and semi-quantitative diagnosis of intestinal helminth infections caused by Ascaris lumbricoides, Trichuris trichiura, hookworm, and Schistosoma spp.

Advantages

Recommended by WHO in areas with moderate to high transmission rates of STH
Widespread tool in intestinal helminth epidemiological surveys
Used in post-treatment surveys to assess impact of control

Limitations

Sensitivity is low, particularly for detection of light-intensity helminth infections

Reporting

Results reported as number of eggs per gram (EPG) of stool
Multiply the number of eggs counted from the slide by a factor (24 for a 41.7 mg template)

Materials Needed

Kato set (template with hole, scree, nylon or plastic, plastic spatula)
Microscope slides
Cellophane soaked in glycerol-malachite green or glycerol-methylene blue solution
Stool sample
Applicator sticks

Procedure

Soak cellophane strips in 50% glycerol-malachite green (or methylene blue) for at least 24 hours before use
Transfer small amount of feces on the newspaper
Press the screen on top of the fecal sample
Remove template carefully, leaving all fecal material on the slide
Cover the fecal sample on the slide with a glycerol-soaked cellophane strip
Invert the microscope slide and press the fecal sample against the cellophane on a smooth surface
Let it sit for 1 hour or more at ambient temperature to clear the fecal material before examination under the microscope

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Description

This quiz covers the Kato Katz technique in clinical parasitology laboratory, including preparation and examination of fecal samples. Learn about the steps involved in this diagnostic process.