Podcast
Questions and Answers
What is the purpose of the mesh screen in the Kato Katz technique?
What is the purpose of the mesh screen in the Kato Katz technique?
- To remove large particles from the fecal sample (correct)
- To remove small particles from the fecal sample
- To mix the feces with glycerol
- To apply pressure to the fecal sample
What is the multiplication factor used if a 41.7 mg template is used?
What is the multiplication factor used if a 41.7 mg template is used?
- 30
- 50
- 24 (correct)
- 20
What is the purpose of soaking the cellophane strips?
What is the purpose of soaking the cellophane strips?
- To increase the visibility of the eggs
- To clear the fecal material around the eggs (correct)
- To preserve the eggs in the fecal sample
- To remove impurities from the fecal sample
What is the unit of measurement used to report the results of the Kato Katz technique?
What is the unit of measurement used to report the results of the Kato Katz technique?
What is the minimum time required to soak the cellophane strips?
What is the minimum time required to soak the cellophane strips?
Which of the following worms can be recognized in the Kato Katz preparation for many months?
Which of the following worms can be recognized in the Kato Katz preparation for many months?
What is the purpose of the template in the Kato Katz technique?
What is the purpose of the template in the Kato Katz technique?
What is the next step after transferring a small amount of feces onto the newspaper?
What is the next step after transferring a small amount of feces onto the newspaper?
What is the purpose of the applicator sticks?
What is the purpose of the applicator sticks?
How many eggs per gram of stool would you report if you found 350 eggs in the Kato Katz technique?
How many eggs per gram of stool would you report if you found 350 eggs in the Kato Katz technique?
What is the primary purpose of the Kato Katz technique?
What is the primary purpose of the Kato Katz technique?
Which of the following helminths is not detected using the Kato Katz technique?
Which of the following helminths is not detected using the Kato Katz technique?
What is the recommended duration for clearing the fecal material prior to examination under the microscope?
What is the recommended duration for clearing the fecal material prior to examination under the microscope?
What is the advantage of using the Kato Katz technique in areas with moderate to high transmission rates of STH?
What is the advantage of using the Kato Katz technique in areas with moderate to high transmission rates of STH?
What is the limitation of the Kato Katz technique?
What is the limitation of the Kato Katz technique?
What is the purpose of inverting the microscope slide and pressing the fecal sample against the cellophane?
What is the purpose of inverting the microscope slide and pressing the fecal sample against the cellophane?
What should be done if an excess of glycerol is present on the upper surface of the cellophane?
What should be done if an excess of glycerol is present on the upper surface of the cellophane?
What is the correct way to handle the slide after pressing the fecal sample against the cellophane?
What is the correct way to handle the slide after pressing the fecal sample against the cellophane?
What is the purpose of the Kato Katz technique in post-treatment surveys?
What is the purpose of the Kato Katz technique in post-treatment surveys?
What is the material used to cover the fecal sample on the slide?
What is the material used to cover the fecal sample on the slide?
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Study Notes
Kato Katz Technique
- Used for qualitative and semi-quantitative diagnosis of intestinal helminth infections caused by Ascaris lumbricoides, Trichuris trichiura, hookworm, and Schistosoma spp.
Advantages
- Recommended by WHO in areas with moderate to high transmission rates of STH
- Widespread tool in intestinal helminth epidemiological surveys
- Used in post-treatment surveys to assess impact of control
Limitations
- Sensitivity is low, particularly for detection of light-intensity helminth infections
Reporting
- Results reported as number of eggs per gram (EPG) of stool
- Multiply the number of eggs counted from the slide by a factor (24 for a 41.7 mg template)
Materials Needed
- Kato set (template with hole, scree, nylon or plastic, plastic spatula)
- Microscope slides
- Cellophane soaked in glycerol-malachite green or glycerol-methylene blue solution
- Stool sample
- Applicator sticks
Procedure
- Soak cellophane strips in 50% glycerol-malachite green (or methylene blue) for at least 24 hours before use
- Transfer small amount of feces on the newspaper
- Press the screen on top of the fecal sample
- Remove template carefully, leaving all fecal material on the slide
- Cover the fecal sample on the slide with a glycerol-soaked cellophane strip
- Invert the microscope slide and press the fecal sample against the cellophane on a smooth surface
- Let it sit for 1 hour or more at ambient temperature to clear the fecal material before examination under the microscope
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