18 Questions
Which type of culture media has an exact known composition due to the addition of precise amounts of purified chemicals to distilled water?
Defined media
What is the characteristic of complex media that sets it apart from defined media?
It employs digests of impure substances
Which type of media is commonly used for the cultivation of fastidious organisms that have complex nutritional requirements?
Enriched medium
A microbiologist is looking to isolate a specific bacterial species from a mixed culture. Which type of media would be most suitable for this purpose?
Selective medium
Which type of culture media is particularly useful for identifying organisms based on their ability to grow under specific conditions or nutritional requirements?
Selective medium
In a laboratory setting, which type of media would be best for distinguishing between colonies of different bacterial species based on their biochemical characteristics?
Differential medium
What distinguishes selective medium from differential medium in microbiology?
Selective medium contains compounds that inhibit the growth of specific microbes, while differential medium contains indicators for specific chemical reactions.
What is the purpose of enriched medium in microbiology?
To culture microorganisms that are difficult to grow due to specific nutritional requirements
Why is it essential to tailor culture media to meet the nutritional requirements of microorganisms?
To support the growth of fastidious microorganisms with specific nutritional needs
How do solid media differ from liquid media in microbiological cultures?
Solid media allow cells to form visible colonies, while liquid media do not
What is the primary purpose of aseptic technique in microbiological practices?
To prevent contamination of culture media with unwanted organisms
Which type of culture contains only a single kind of microbe?
Pure culture
What is the purpose of aseptic technique in microbiology?
To prevent contamination during manipulations of cultures and sterile culture media
What makes endospores highly resistant to heat, harsh chemicals, and radiation?
Their differentiation into a dormant stage
Why are airborne contaminants a common problem in microbiology laboratories?
Because the dust in laboratory air contains microorganisms
Which statement best describes the life cycle of endospore-forming bacteria?
Vegetative cell -> endospore -> vegetative cell
What triggers endospore formation in bacteria?
Exhaustion of an essential nutrient
Where are endospore-forming bacteria commonly found?
In soil
Learn about enriched, selective, and differential culture media used in microbiology to culture different types of microorganisms. Discover how these media help in growing fastidious organisms and detecting specific chemical reactions during growth.
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