Classifications of Culture Media: Selective Medium
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Questions and Answers

Which classification of culture media is based on the presence or absence of yeast, plant, or animal tissue?

  • Synthetic Media (correct)
  • Non-Synthetic Media
  • Liquefiable solid media
  • Solid Media

Which type of solid media allows the growth of bacteria as colonies by streaking on the medium?

  • Non-Liquefiable solid media (correct)
  • Liquefiable solid media
  • Synthetic Media
  • Complex media

Which type of culture media is specifically used for the profuse growth of microorganisms and fermentation studies?

  • Transport media
  • Enrichment media (correct)
  • General purpose media
  • Selective media

Which classification of culture media contains ingredients that are not represented by an exact chemical formula?

<p>Non-Synthetic Media (A)</p> Signup and view all the answers

Which type of culture media does not solidify at temperatures above the freezing point and can lead to the formation of a 'surface pellicle' by certain bacteria?

<p>Liquid media (A)</p> Signup and view all the answers

Which type of culture media is entirely free of animal-derived components and useful for studying bacterial physiology and metabolic nature?

<p>Synthetic Media (B)</p> Signup and view all the answers

What is the purpose of semi-solid media prepared with lower agar concentrations?

<p>To determine bacterial motility (C)</p> Signup and view all the answers

Which classification of culture media focuses on promoting the growth of a specific group of microorganisms while inhibiting others?

<p>Selective media (C)</p> Signup and view all the answers

Which classification of culture media is utilized to identify a specific organism based on staining procedures?

<p>Differential media (D)</p> Signup and view all the answers

Which component in Thayer-Martin Agar inhibits gram-positive bacteria?

<p>Vancomycin (A)</p> Signup and view all the answers

What type of bacteria is Mannitol Salt Agar used for the isolation of?

<p>Staphylococcus aureus (A)</p> Signup and view all the answers

In which type of culture media can certain aerobic bacteria grow as a thin film called 'surface pellicle'?

<p>Liquid media (A)</p> Signup and view all the answers

What makes Lowenstein-Jensen Medium selective?

<p>Malachite green dye (D)</p> Signup and view all the answers

Which agar promotes the growth of gram-negative bacteria and inhibits gram-positive bacteria?

<p>MacConkey’s Agar (A)</p> Signup and view all the answers

What is the purpose of Saboraud’s Dextrose Agar?

<p>Promotion of fungal growth (A)</p> Signup and view all the answers

Which agar is recommended for use in qualitative procedures for the isolation of Neisseria gonorrhoeae and Neisseria meningitidis?

<p>Thayer-Martin Agar (C)</p> Signup and view all the answers

Study Notes

Classification of Culture Media by Functional Type

  • Selective Media: allows the growth of one class of bacteria while inhibiting others
    • Examples: Thayer-Martin Agar, Mannitol Salt Agar, MacConkey's Agar, Lowenstein-Jensen Medium, Saboraud's Dextrose Agar
  • Differential Media: designed to show visible differences among certain groups of microorganisms
    • Examples: MacConkey's Agar (promotes growth of gram-negative bacteria and inhibits gram-positive bacteria)
  • Culture Media: a mixture of substances that promotes and supports the growth and differentiation of microorganisms

Purpose of Culture Media

  • To obtain pure cultures
  • To grow and count microbial cells
  • To cultivate and select microorganisms
  • To identify specific organisms based on staining procedures

Classification of Culture Media by Physical State

  • Solid Media: contains a solidifying agent such as agar, allowing bacteria to form discrete colonies
    • TWO FORMS: Liquefiable solid media (reversible) and Non-Liquefiable solid media (non-reversible)
  • Liquid Media: water-based solutions that do not solidify at temperatures above the freezing point
    • Also called broths, milk, or infusions
    • Used for profuse growth of microorganisms and fermentation studies
  • Semi-Solid Media: prepared with lower agar concentrations of 0.5%
    • Used to cultivate microaerophilic bacteria or determine bacterial motility

Classification of Culture Media by Chemical Composition

  • Synthetic Media: defined media with no yeast, plant, or animal tissue present, and all chemicals are known
    • Made from pure ingredients with exact composition known
    • Useful for studying physiology, metabolic nature, and nutritional requirements of bacteria
  • Non-Synthetic Media: complex media containing at least one ingredient that is not chemically defined
    • Contain extracts of animals, plants, or yeasts
    • Support the growth of more fastidious organisms

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Learn about the functional type of culture media known as selective medium, which allows the growth of specific bacteria while inhibiting others. Explore examples such as Thayer-Martin Agar and its use in isolating Neisseria gonorrhoeae and Neisseria meningitidis.

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