18 Questions
What is a colony in microbiology?
A visible collection of millions of bacteria from a single bacterial cell
Who discovered agar as a solidifying agent in culture media?
Frau Hesse
Why is gelatin not considered satisfactory for use in culture media?
It liquefies at 24ºC
What is the purpose of synthetic or defined media in microbiology?
To provide trace elements and vitamins required by microorganisms
Which of the following is often used as an inorganic nitrogen source in culture media?
Ammonium salts
What is the main purpose of using liquid (broth) culture media?
To promote profuse growth
What is the purpose of an autoclave in a laboratory setting?
To sterilize equipment and objects by using steam
How does an autoclave work to achieve sterilization?
By maintaining a high pressure and allowing steam to enter
What is the function of an incubator in a microbiology laboratory?
To maintain optimal conditions for growing microbiological cultures
Why might a microwave be used in a microbiology laboratory for media preparation?
To save time and ensure media quality
What is the purpose of a laminar flow hood in a laboratory setting?
To bathe the work area with positive pressure HEPA-filtered air
How can a refrigerator be utilized in a laboratory?
To preserve certain chemical reagents or media
Which specialized tool is commonly used for spreading bacteria in the plate?
L-Shape Glass Rods
What is the main purpose of using an Inoculating Needle?
To determine the shape of the hole digested by bacteria
Which specialized tool is NOT recommended for culturing bacteria due to its wide mouth?
Beaker
What is the function of Mechanical Pipettes?
To extract or deliver small amounts of liquid
Which specialized tool is used for incineration to avoid contaminants?
Alcohol Lamp
For what purpose would you use a Cotton Swab instead of an Inoculating Loop?
During sampling
Test your knowledge on culture media used in microbiology for growing bacteria and preserving cultures. Learn about the history of solid mediums like agar and gelatin. Explore the concept of bacterial colonies and their origins.
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