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Questions and Answers
Match the following bacteria with their preferred growth temperatures:
Campylobacter jejuni = 42°C Listeria monocytogenes = cold enrichment Yersinia enterocolitica = room temperature Neisseria = 35-37°C
Match the following bacteria with their preferred growth pH:
Helicobacter pylori = acidic pH Neisseria = neutral pH (6.5-7.5) Moraxella = alkaline pH Wound bacteria = variable pH
Match the following Gram stain errors with their causes:
Overdecolorization = decolorizer sits on too long Undercolorization = decolorizer rinse off too quickly False g-ves = incorrect order of steps Damaged cell walls = cultures too old/antibiotics
Match the following bacteria growth requirements with their descriptions:
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Match the following media with their characteristics:
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Match the following bacterial cell wall types with their characteristics:
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Match the following types of media with their characteristics:
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Match the following specimens with the media used:
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Match the following microorganisms with their characteristics:
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Match the following microbes with their types:
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Match the following terms with their definitions:
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Match the following media with their uses:
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Match the following bacterial characteristics with their corresponding environments:
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Match the following bacterial shapes with their corresponding Greek meanings:
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Match the following hemolysis types with their corresponding effects on red blood cells:
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Match the following bacterial nutrition preferences with their corresponding food sources:
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Match the following bacterial identification methods with their corresponding techniques:
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Match the following bacterial species with their corresponding characteristics:
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Match the following forms of antibiotics with their routes of administration:
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Match the following terms with their definitions in the context of antibiotic sensitivity testing:
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Match the following statements with their implications on antibiotic treatment:
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Match the following challenges with their context in antibiotic development:
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Match the following statements with their context in the history of antibiotics:
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Match the following terms with their descriptions in the context of antibiotic sensitivity testing:
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Study Notes
Neisseria/Moraxella Wound MacConkey Agar (MAC)
- Selective media for lactose fermentation, with a pH indicator (neutral red) and inhibitors (crystal violet and bile salts) for Gram-positive bacteria
- Specimens include urine, blood, respiratory, genital, and wound samples
Basic Equipment
- Loops (reusable or disposable) and incinerators for aseptic technique
- Media (solid or liquid) for growth requirements
- Aspectic technique practiced to prevent contamination
Growth Requirements
- Air: aerobic, anaerobic, or microaerobic environments
- CO2 levels: some require increased CO2 (e.g., Campylobacter)
- Temperature: 35-37°C (some species require different temperatures, e.g., Listeria monocytogenes at 4°C)
- pH: most prefer neutral pH (6.5-7.5), but some are acidophiles (e.g., Helicobacter pylori)
Gram Stain
- Differential stain to identify Gram-positive and Gram-negative bacteria
- Errors can occur due to over- or under-decolorization, incorrect order of steps, or damaged cell walls
- Mixed cultures can lead to false results
Antibiotic Sensitivity Testing
- Inoculate plate with bacterial suspension and add antibiotic discs
- Clear zone around disc indicates sensitivity, while growth around the disc indicates resistance
Microbiology
- Importance of microbiology in patient care, antimicrobial resistance, and antibiotic development
- Objectives include describing microbiology plate differences, quadrant streaking, Gram stain, and the importance of microbe identification for patient care
Bacteria Cell Walls
- About 90% of bacteria have a cell wall, with two main types: Gram-positive and Gram-negative
- Gram stain identifies cell wall types, with potential errors due to over- or under-decolorization
Microbe Identification
- Importance of identification for patient care, antibiotic sensitivity testing, and antimicrobial resistance
- Rapid microbe identification using MALDI-TOF and genetic analysis for infection control and epidemiological studies
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Description
Test your knowledge of microbiology, including the importance of microbe identification, gram staining, and sensitivity testing. Learn about different types of microbes, including bacteria, viruses, fungi, and parasites, and how to identify them. Understand the significance of microbiology in patient care.