Podcast
Questions and Answers
What is the definition of Microbiology?
What is the definition of Microbiology?
The study of microorganisms
What is the name of the first antibiotic?
What is the name of the first antibiotic?
Penicillin
Prions are easily killed by sterilization?
Prions are easily killed by sterilization?
False (B)
Which of the following requirements of cell life is best described by the preferred temperature of 37 deg C.?
Which of the following requirements of cell life is best described by the preferred temperature of 37 deg C.?
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Transient flora is always found on the body?
Transient flora is always found on the body?
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Which statement regarding the sterile field is FALSE?
Which statement regarding the sterile field is FALSE?
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Remitting symptoms are long-lasting and recurrent.
Remitting symptoms are long-lasting and recurrent.
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What stage of illness is characterized by a person starting to feel tired and having a sore throat?
What stage of illness is characterized by a person starting to feel tired and having a sore throat?
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An HAI is acquired prior to hospitalization.
An HAI is acquired prior to hospitalization.
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From the following list, which type of HAI is most influenced by MDR?
From the following list, which type of HAI is most influenced by MDR?
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What is the best description of the action of Efflux pumps?
What is the best description of the action of Efflux pumps?
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Medical asepsis is also known as the clean technique?
Medical asepsis is also known as the clean technique?
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Surgical asepsis techniques are only performed in operating rooms.
Surgical asepsis techniques are only performed in operating rooms.
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Environmental cleaning is an example of medical asepsis.
Environmental cleaning is an example of medical asepsis.
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Masks and caps used in sterile procedures are sterile.
Masks and caps used in sterile procedures are sterile.
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Infectious disease may be spread during the incubation period?
Infectious disease may be spread during the incubation period?
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We are born with adaptive immunity.
We are born with adaptive immunity.
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Macrophages recognize PAMPS because of PRRs (external molecules on the surface of the macrophage.)?
Macrophages recognize PAMPS because of PRRs (external molecules on the surface of the macrophage.)?
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A cytokine storm can cause damage to the epithelial tissue of the lung?
A cytokine storm can cause damage to the epithelial tissue of the lung?
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Provide 2 conditions which increase the susceptibility of a host.
Provide 2 conditions which increase the susceptibility of a host.
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Which mode of transmission is easiest to break?
Which mode of transmission is easiest to break?
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Who is considered the "father of modern microbiology"?
Who is considered the "father of modern microbiology"?
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Flashcards
What is Microbiology?
What is Microbiology?
Study of microorganisms, typically less than 1 mm in diameter, requiring a microscope for visibility. Includes viruses, bacteria, fungi, algae, and protozoa.
Microbiology Specialties
Microbiology Specialties
Specialized fields within microbiology focusing on specific types of organisms.
Microbiology Fields/Work
Microbiology Fields/Work
Areas of work in microbiology with a more applied focus.
What is Cell Theory?
What is Cell Theory?
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Five Characteristics of All Cells
Five Characteristics of All Cells
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What is a Prokaryotic Cell?
What is a Prokaryotic Cell?
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What is a Eukaryotic Cell?
What is a Eukaryotic Cell?
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Cell Membrane
Cell Membrane
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Cytoplasm
Cytoplasm
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Nucleus/Nucleoid
Nucleus/Nucleoid
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Organelles
Organelles
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Cell Wall
Cell Wall
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Spore Coat
Spore Coat
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Proteins
Proteins
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What is Taxonomy?
What is Taxonomy?
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Nomenclature
Nomenclature
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What is Morphology?
What is Morphology?
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Macroscopic Morphology
Macroscopic Morphology
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Microscopic Morphology
Microscopic Morphology
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Aerobes
Aerobes
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Anaerobes
Anaerobes
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Facultative Anaerobes
Facultative Anaerobes
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Gram Stain
Gram Stain
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What are Bacteria?
What are Bacteria?
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What are Viruses?
What are Viruses?
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What are Fungi?
What are Fungi?
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What are Prions?
What are Prions?
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Lipophilic Viruses
Lipophilic Viruses
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Hydrophilic Viruses
Hydrophilic Viruses
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What is Normal Flora?
What is Normal Flora?
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Resident Flora
Resident Flora
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Transient Flora
Transient Flora
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Sterile Body Tissues
Sterile Body Tissues
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Advantages of Normal Flora
Advantages of Normal Flora
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Disadvantages of Normal Flora
Disadvantages of Normal Flora
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Mutualistic Relationship
Mutualistic Relationship
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Commensalistic Relationship
Commensalistic Relationship
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Parasitic Relationship
Parasitic Relationship
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What is Pathogenesis?
What is Pathogenesis?
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Contamination
Contamination
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Infection
Infection
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Disease
Disease
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Portals of Entry
Portals of Entry
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Symptoms
Symptoms
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Signs
Signs
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Syndrome
Syndrome
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Asymptomatic Infection
Asymptomatic Infection
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What is Etiology?
What is Etiology?
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Pathogenicity
Pathogenicity
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Virulence Factors
Virulence Factors
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Adhesion
Adhesion
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Biofilm
Biofilm
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Extracellular Pathogens
Extracellular Pathogens
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Intracellular Pathogens
Intracellular Pathogens
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Systemic Spread
Systemic Spread
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Tissue Injury
Tissue Injury
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Exotoxins
Exotoxins
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Endotoxins
Endotoxins
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Specific Immunity
Specific Immunity
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What is Epidemiology?
What is Epidemiology?
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Determinants
Determinants
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Inclusion/Exclusion Criteria
Inclusion/Exclusion Criteria
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Prevalence
Prevalence
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Incidence
Incidence
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Epidemic
Epidemic
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Surveillance
Surveillance
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Active Surveillance
Active Surveillance
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Passive Surveillance
Passive Surveillance
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Notifiable Conditions
Notifiable Conditions
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Pandemic
Pandemic
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Prognostic Signs
Prognostic Signs
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Anamnestic Signs
Anamnestic Signs
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Diagnostic Signs
Diagnostic Signs
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Pathognomonic Signs
Pathognomonic Signs
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Remitting Symptoms
Remitting Symptoms
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Chronic Symptoms
Chronic Symptoms
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Relapsing Symptoms
Relapsing Symptoms
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Incubation Period
Incubation Period
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Prodromal Period
Prodromal Period
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Period of Illness
Period of Illness
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Period of Decline
Period of Decline
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Period of Convalescence
Period of Convalescence
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Physical and Chemical Barriers
Physical and Chemical Barriers
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Innate Immunity
Innate Immunity
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Pathogen Recognition
Pathogen Recognition
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PAMP's
PAMP's
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PRRs
PRRs
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Macrophages
Macrophages
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Cytokines
Cytokines
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Cytokine Storm
Cytokine Storm
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Phagocytosis
Phagocytosis
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Inflammation
Inflammation
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Natural Killer (NK) Cells
Natural Killer (NK) Cells
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Complement System
Complement System
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Clean
Clean
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Disinfected
Disinfected
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Sterilization
Sterilization
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Asepsis
Asepsis
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Medical Asepsis
Medical Asepsis
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Surgical Asepsis
Surgical Asepsis
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Routine Practices
Routine Practices
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Additional Precautions
Additional Precautions
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Contact Precautions
Contact Precautions
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Droplet Precautions
Droplet Precautions
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Droplet/Contact Precautions
Droplet/Contact Precautions
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Airborne Precautions
Airborne Precautions
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Infection Control Strategies
Infection Control Strategies
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Health Care Associated Infections (HAI's)
Health Care Associated Infections (HAI's)
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Point of Care Risk Assessment (PCRA)
Point of Care Risk Assessment (PCRA)
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Environmental/Engineering Controls
Environmental/Engineering Controls
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Administrative Controls
Administrative Controls
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What is the Public Health Agency of Canada (PHAC)?
What is the Public Health Agency of Canada (PHAC)?
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Vaccination
Vaccination
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Live-Attenuated Vaccines
Live-Attenuated Vaccines
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Inactivated Vaccines
Inactivated Vaccines
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Subunit Vaccines
Subunit Vaccines
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Viral Vector Vaccines
Viral Vector Vaccines
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Antibiotic Resistance
Antibiotic Resistance
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Impact of Sanitation, Vaccination, and Antibiotics on Life Expectancy
Impact of Sanitation, Vaccination, and Antibiotics on Life Expectancy
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Strategies for Antibiotic Resistance
Strategies for Antibiotic Resistance
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Cleaning
Cleaning
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Autoclaving
Autoclaving
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Radiation Sterilization
Radiation Sterilization
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Chemical Sterilization
Chemical Sterilization
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Study Notes
RRC Polytech
- RRC Polytech is an institution, likely a college or vocational school.
Recording Alert
- This session is being recorded.
- Recordings will be available for future RRC Polytech members.
Land Acknowledgment
- A slide with images of natural scenes.
Microbiology and Infection Control
- This is a final review of the microbiology and infection control course.
Learning Outcomes
- Students should be able to identify important events in the History of Microbiology.
- Students should be able to define microorganisms and list three classifications.
- Students should be able to tell the difference between normal flora and pathogens.
- Students should be able to identify the ideal environment for the growth of microorganisms.
- Students should be able to recognize and describe the body's natural defense mechanisms.
- Students should be able to describe the chain of infection.
- Students should be able to identify portals of entry to the human body and those of exit.
- Students should be able to recognize and describe modes of transmission.
- Students should be able to identify factors that increase individual susceptibility to infection.
- Students should be able to list signs and symptoms of infection.
- Students should be able to define healthcare-associated infection (HAI).
- Students should be able to distinguish between clean, dirty, and sterile objects.
- Students should be able to define medical asepsis.
- Students should be able to describe the major Infection Control Strategies.
Module 1: Objectives
- Define Microbiology
- List techniques used to isolate and culture microorganisms
- Identify and define different types of microbiological specialties.
- Identify areas of interest for microbiological fields/work
- Name and describe the contributions of major historical figures in microbiology, and their modern-day impacts.
What is Microbiology?
- The study of microorganisms typically smaller than 1mm in diameter.
- Examples include viruses, bacteria, fungi, algae, and protozoa.
What is Microbiology? Specialties
- Microbiology can focus on specific types of organisms:
- Virology - viruses
- Bacteriology - bacteria
- Phycology - algae
- Mycology - fungi
- Protozoology - protozoa
What is Microbiology? Fields/Work
- Microbiological work can focus more on applied sciences:
- Medical microbiology, including immunology
- Food and Dairy microbiology
- Public Health microbiology (Epidemiology)
- Industrial microbiology
Historical Timeline 1665-1995: Study Example
- A timeline showcasing key developments in microbiology from 1665-1995.
Module 2: Cell Structure of Microorganisms
- Taxonomy/Morphology
- Types of Microorganisms
Module 2 Objectives
- Define cell theory
- List 5 characteristics of all cells
- Identify cell structure basic components: the cell membrane, cytoplasm, nucleus/nucleoid, organelles, cell wall, and spore coat.
- Distinguish between eukaryotic and prokaryotic cells
- Define protein impact on MDR
- Describe the characteristics of proteins
- Define taxonomy and the nomenclature method for bacteria- genus and species
- Define morphology and macroscopic and microscopic morphology implications
- Define anaerobic, aerobic, and facultative anaerobe
- Describe bacterial shapes and give examples
- Outline structural differences between Gram+ and Gram- bacteria & human impact
- Describe the significance and procedure for Gram staining
Cell Theory
- All organisms are formed of one or more cells and all cells are derived from pre-existing cells.
- Cells are the basic units of life and common to all living things.
5 Characteristics of All Cells
- Self Feeding/Nutrition
- Self Replication/Growth
- Differentiation of forming new cell structures
- Chemical Signalling/Communication
- Evolution/Changes to show new biological properties
Types of Cells
- Prokaryotic Cells
- No nucleus or other membrane-bound organelles
- Usually unicellular
- Eukaryotic Cells
- Has a nucleus and multiple complex membrane-bound organelles
- Can be unicellular or multicellular
Differences Between Prokaryotic and Eukaryotic Cells
- Genetic Information
- Organelles
- Size
- Organisms
- Cell Structure
Cell Membrane
- The "skin" of the cell, providing protection by regulating the movement of water, nutrients and waste into and out of the cell.
Cytoplasm
- The largest part of the cell, made up of water and proteins in a gel-like consistency.
- All cell activities, function, growth and replication happen here, though the nucleus coordinates the actions.
Nucleus/Nucleoid
- The cell's "brain".
- Contains the genetic information to direct cell behavior and appearance function.
Organelles
- Includes the nucleus and several other structures that perform specific functions within the cell.
Cell Wall
- Most, but not all bacteria, have a rigid cell wall.
- In plants, it helps the cell maintain its shape.
- It does not replace the cell membrane
Spore Coat
- A protective layer for some microorganisms.
- Allows for bacterial rest and protection in unfavorable conditions.
Proteins
- Proteins are a major component of cytoplasm and form the human body and its waste matter.
- Common elements within the waste sent to the MDRD laboratory.
Characteristics of Protein
- Not soluble in water
- Can be coagulated by heat, drying, or specific chemicals
- Coagulated protein can trap and protect pathogens.
- Must be removed prior to disinfection or sterilization.
Blood: Source of Protein on Instruments
- Blood sample types and images of instruments commonly used in medical/surgical contexts.
Taxonomy: Genus/Species
- A table listing genus and related species.
Macroscopic Morphology
- Image of bacterial growth on a plate.
- The visual appearance of the bacteria can be observed.
Microscopic Morphology
- Requirements for oxygen for bacteria growth
- Gram Stain procedure.
- Bacterial shape and groupings.
Gram Stain: Method
- Gram + vs. Gram – bacteria stained using a four step procedure involving crystal violet, gram's iodine, an alcohol decolorizer and a safranin counterstain.
Microscopic Morphology
- Bacterial morphology in forms of cocci, bacilli, helical form.
Classification of Microorganisms
- List of microorganisms that are broadly categorized from bacteria to prions.
Bacteria
- Unicellular organisms
- Present in every habitat
- Outnumber human cells.
- Less than 1% are harmful (pathogenic)
Viruses
- Smaller than bacteria
- Cannot replicate independently of host cells.
Fungi
- Molds and Yeasts:
- Microscopic/Large organisms
- Crucial in food production (bread)
- Can cause disease and allergic reactions.
Prions
- Infectious proteinaceous particles.
- Misfolded proteins that cause similar body proteins to deform.
Viruses: Types
- Lipophilic viruses have an envelope, are more easily killed by hospital disinfectants, and can be very pathogenic.
- Hydrophilic viruses have no envelope.
Bacteria: Interventions
- May be susceptible to antibiotics and immunization.
- Disinfectants/sterilization may also be effective (with spore forming bacteria requiring sterilization).
Viruses: Interventions
- Viruses are not susceptible to antibiotics.
- Prevention via vaccines are paramount
- Disinfectants can reduce the amount and type of viruses.
Fungi: Interventions
- Fungal infections becoming more prevalent in modern medicine.
- Treatment via antifungals (topical, oral or IV)
Prions: Interventions
- Prions can remain infectious for years; no cure; cannot be destroyed by standard sterilization methods, radiation, boiling or alcohol.
Module 3: Normal and Pathogenic Organisms
- Symbiotic Relationships
- Pathogenesis - the Origin of Disease
Module 3: Objectives
- Define the human microbiome
- Define normal flora and describe its benefits
- Define resident and transient flora
- List sterile body tissues
- List locations where normal flora are found
- List factors influencing resident flora
- List disadvantages of normal flora
- Define and give examples of symbiotic relationships (mutualistic, commensalistic, opportunistic)
- Define pathogenesis.
- Define contamination vs. infection.
- List the main portals of entry.
- Define infection vs. disease
- Define asymptomatic/subclinical infections
- Define etiology
- Define pathogenesis vs. pathogenicity
- List and define the factors affecting pathogenicity (virulence, virulence factors)
- Describe mechanism of pathogenicity (adhesion, biofilm, extra/intracellular, systemic, tissue injury)
- List and define three causes of tissue injury (exotoxin, endotoxin, specific immunity)
Human Microbiome: Definition
- The collection of microorganisms residing on the surface/deep layers of skin, oral mucosa, conjunctiva, and the gastrointestinal tract.
Normal Flora: Definition
- A mixed population of microorganisms (bacteria and fungi) regularly found at various anatomical sites within the human body.
Normal Flora: Advantages
- Provide some essential nutrients (e.g., vitamin K).
- Stimulate the immune system.
- Prevent colonization by potential pathogens.
- Inhibit some pathogens.
Normal Microbial Flora: Types
- Resident Flora
- Microbes constantly present.
- Transient Flora
- Microbes reside temporarily (hours to months).
Normal Flora
- Expected/Normal (Skin, Eyes, Nose, Mouth, EARS, Urogenital Tract/Digestive Tract).
- Not Expected/Abnormal (Blood, Brain, Muscle, Cerebrospinal Fluid)
Importance of Normal Flora (Advantages)
- Constitute a protective host defense mechanism by occupying ecological niches.
- Produce vitamin B and K in the intestines.
- Contribute to immunity by inducing low levels of circulating and secretory antibodies.
Importance of Normal Flora (Disadvantages)
- Can cause disease when individuals become immunocompromised or their usual anatomic location shifts.
Normal Flora: Influencing Factors
- Age (changes continuously throughout life)
- Nutrition
- Environment
- Sex
The Three Types of Symbiotic Relationships
- Mutualism (both organisms benefit)
- Commensalism ( one organism benefits, the other isn't harmed or benefited)
- Parasitism (one organism benefits, the other is harmed.)
Module 3. Pathogenesis
- The origin and development of disease (contamination, infection, disease).
Contamination vs. Infection
- Contamination - the mere presence of microbes.
- Infection - microbes evade defenses, multiply, and establish in the body.
Infection vs. Disease
- Infection - invasion and establishment of a pathogen.
- Disease - infection alters normal body functions.
Portals of Entry
- Skin
- Mucous membranes
- Placenta
- Parenteral route
Manifestations of Disease
- Symptoms (subjective)
- Signs (objective)
- Syndrome (group of symptoms)
Asymptomatic Infection
- Lacking symptoms but with signs of infection.
Etiology: Definition
- Study of the cause of disease.
- Germ theory of disease: disease is caused by infections/pathogens.
Pathogenesis vs. Pathogenicity
- Pathogenesis - origin and development of disease.
- Pathogenicity - the ability of an organism to cause disease.
Definitions
- Pathogenesis - the origin and development of disease
- Virulence - the ability of a microorganism to cause disease
- Virulence Factors – the molecules expressed and secreted that aid in pathogenesis.
Pathogenicity: Mechanism
- Adhesion, how bacteria attach to tissues
- Biofilm, formation of a collective of one or more types of microorganisms, comprising polysaccaride web (slime) and biosensing.
After Infection: Penetration & Spread/Tissue Injury
- Exotoxin - Secreted by certain bacteria
- Endotoxin vs Specific Immune Response - Microbial Toxins, when bacterial cells lyse and specific immune system response
Exotoxins
- Proteins
- Secreted by bacteria
- Destroy cell membranes
- Examples: Diphtheria Toxin, Cholera Toxin, Tetanus Toxin
Endotoxins
- Can lead to endotoxic shock, a severe reaction associated with hypotension, fever, and intravascular coagulation
- Potentially leading to organ failure or death (SEPSIS)
Specific Immunity
- Continuous secretion of antigens stimulates the immune system
- Example: Rheumatic Fever
- Muscle damage caused by hypersensitivity reaction
Module 4: The Chain of Infection
- Introduction to Epidemiology
Module 4: Objectives
- Identify and define each link of the chain of infection
- Define epidemiology
- Describe the role of public health in disease assessment, intervention, and reassessment
- Define and describe disease distribution (person, place, time), determinants (risk factors).
- Define inclusion/exclusion criteria.
- Define and describe disease frequency- counts, incidence, prevalence
- Describe the purpose of surveillance and its relationship to epidemiology (early warning, intervention impact)
- Define active vs passive surveillance
- Define and describe notifiable conditions and methods for their reporting.
- Define Pandemic
Chain of Infection
- Infectious Agent
- Reservoir (where the agent can survive and thrive - human, animal, or equipment)
- Portal of Exit (source from which pathogen emerges - blood, respiratory tract, or contaminated equipment/supplies).
- Mode of Transmission (method of transfer from portal of exit to portal of entry).
- Portal of Entry (pathway into a susceptible host - e.g., airway, wound, body insertion
- Susceptible Host (factors influencing the host's susceptibility to infection - e.g., age, chronic disease, or immunosuppression)
Module 5: Pathology - the Disease Process
- Signs and Symptoms of Disease
- Stages of Illness
- The Immune Response – Health Care Associated/Acquired Infection (HAI)
Module 5: Objectives
- Define and provide examples of sign classifications (prognostic, anamnestic, diagnostic, and pathognomonic)
- Describe examples of symptom classifications (remitting, chronic, and relapsing)
- Recognize the stages of illness (incubation, prodromal, illness, decline, convalescence)
- Describe the physical and chemical immune barriers (skin, tears/mucous, pH/blood proteins, flushing)
- Explain immediate and induced innate immune responses (inflammation, pathogen engulfment, secretion)
- Describe innate immunity
- Describe pathogen recognition (PAMPs/PRRs, macrophages, cytokines/cytokine storm)
- Describe phagocytosis and inflammation.
- Define and describe natural killer cells
- Define and describe the complement system
Signs & Symptoms - Review
- Signs/Objective Observations: use of senses, measurable
- Symptoms/Subjective Perceptions: patient experience, descriptive, not measurable.
Signs- Examples
- Fever, Rash, High/Low Blood Pressure, Vomiting, Diarrhea, Hemoptysis, Hematuria
Signs - Classifications
- Prognostic: predicts future outcomes
- Anamnestic: points to past medical history - Example- skin scars from past acne
- Diagnostic: aids in identifying current health problems - High PSA levels in males as a diagnostic sign of prostate cancer
- Pathognomonic: unambiguously links the sign to a condition - Presence of specific bacteria in samples
Symptoms - Classifications
- Remitting: completely resolve - e.g., common cold
- Chronic: long lasting or recurrent - e.g., asthma
- Relapsing: previously resolved but recur - e.g., depression
Symptoms
- Feeling hot/cold
- Pain
- Fatigue
- Muscle aches
- Nausea
Signs & Symptoms
- Foundation of pathology - study of disease
Stages of Illness
- Incubation Period
- Prodromal Period
- Period of Illness (acme point)
- Period of Decline
- Period of Convalescence
Stages: Incubation
- Microbes infect, multiply, individual doesn’t feel sick, microbes may be easily spread.
- Length depends on the microbe and individual’s immune system
Stages: Prodromal
- Short stage where individual starts to feel sick
- Brain aware of a problem, not sure what exactly
Stages: Period of Illness
- Individual feels typical signs and symptoms of disease.
- Acme point is the peak intensity of the illness – most spread is during this stage, if communicable
Stages: Period of Decline
- Immune system controls microbes, clinical signs/symptoms lessen.
Stages: Period of Convalescence
- Microbial replication fully stops.
- Individual returns to pre-illness state.
- Still possible to transmit the disease in some cases.
The Immune Response ("Fighting Back")
- Physical and Chemical Barriers
- Innate immunity
- Pathogen Recognition
Conditions that May Overcome Physical/Chemical Barriers
- Skin abrasions/puncture
- Large numbers of pathogen that can overcome mucus/cilia
- Evolution of specific mechanisms of the pathogen
Innate Immunity
- Inflammation initiates when pathogens enter the body
- Pathogen engulfment
- Secretion of immune factors and proteins
Innate Immunity - Characteristics
- Natural
- Developed/evolved early
- Not induced by infection/vaccination
- Limited number of targets
Pathogen Recognition
- Blood/lymph cells recognize specific PAMPS (pathogen associated molecular patterns) on pathogen surfaces
- Macrophages engulf foreign particles/pathogens
- Macrophages recognize PAMPS via PRRS (pathogen recognition receptors)
- Binding of PAMPS/PRRs releases cytokines
Cytokines
- Chemical messengers
- Regulate cell differentiation/proliferation/gene expression for immune response.
- Signs of feeling sick (e.g., lethargy, muscle pain, nausea)
- Encourage rest/prevent spreading
Cytokine "Storm"
- Normally part of the body’s immune response to infection.
- Sudden release in large quantities potentially leading to multisystem organ failure/death.
Macrophages and Natural Killer Cells
- Macrophages engulf pathogens, stimulate immune responses in other cells.
- Natural Killer cells kill infected cells or cancer cells, constantly patrolling the body
Summary: Phagocytosis/Inflammation
- Response to injury/cuts
- Histamine secretion, causing dilation of capillaries.
- Neutrophils/monocytes leave capillaries, monocytes become macrophages
- Release chemicals to trigger inflammatory response.
- Phagocytosis by neutrophils/macrophages
Natural Killer (NK) Cells
- Always functioning/active
- Patrol the body to control potential infections and stop cancer progression
Review: Innate Immune System
- First responders
- Employ inflammation, phagocytosis, cytokine release, destruction by NK cells, and/or complement system.
- Adaptive system is informed when innate mechanisms are insufficient to handle an infection.
Review: Innate Immune System (Functions/Characteristics)
- Functions: inflammation, pathogen engulfment, secretion of immune factors and proteins
- Characteristics: natural immunity - developed/evolved early, not induced by infection/vaccination, limited # of targets
Review: Pathogen Recognition
- Viruses: intracellular
- Bacteria: intracellular/extracellular
- PAMPS – present on pathogen surfaces
- PRRS – receptors on macrophages
Review: Cytokines
- Cytokine release effect = PAMPS + PRRS
- Chemical messengers: cell differentiation (form/function), proliferation (production), gene expression.
- Causes illness symptoms – fever, etc
- Cytokine Storm: uncontrolled, excessive cytokine release leads to multiorgan failure/death.
Review: Phagocytosis/Inflammation
- WBCs release chemicals to gather at infection site (necessary for all cells)
- Can develop hypersensitivity/allergic response
Review: NK's and Complement System
- Natural Killer cells - lymphocytes patrolling the body to destroy infected and tumor cells
- Complement proteins – assist in destroying extracellular pathogens/compliment antibody response
- Adaptive Immune System
Module 6: Clinical Applications of MDR
- Clinical applications of multi-drug resistant organisms (MDR).
- MDR Basics: Clean vs. Disinfected vs. Sterile
- Medical, Surgical Asepsis
- Routine Practices
- Additional Precautions/Disease Transmission
- Infection Control Strategies
- Antibiotic Resistance
- HAI's
Module 6: Objectives
- Define cleaning, disinfection, and sterilization
- Define asepsis
- Listing 3 examples of medical asepsis (respiratory, environmental cleaning, PPE)
- Define surgical asepsis
- Components of surgical asepsis (equipment sterilization, sterile fields, sterile workers, sterile techniques)
- Examples of breaches in sterile technique
- Define routine practices
- Define PHAC
MDR Basics
- Clean: no visible soil; reduces microbial load.
- Disinfected: reduces microbes to 99%.
- Sterility: one in a million chance of a viable organism.
Asepsis
- Freedom from living pathogenic organisms.
- Techniques don’t discriminate between pathogens and non-pathogens (they kill or avoid)
Basics of Medical Asepsis
- Practices designed to prevent infection spread.
- Also called 'clean technique’ - Example- Hand Hygiene
Examples of Medical Asepsis
- Respiratory Etiquette/Hygiene
- Environmental Cleaning
- Personal Protective Equipment (PPE)
What is Surgical Asepsis?
- Ensuring a completely microbe-free environment.
- Used with surgical/brief invasive procedures that breech skin or mucous membranes, or normally sterile parts of the body
Components of Surgical Asepsis
- Equipment Sterilization
- Sterile Field
- Sterile Workers
- Sterile Technique
Breaches in Sterile Technique
- Sterile field exposure to air.
- Coughing/sneezing over sterile field.
- Talking excessively over sterile field
- Unsterile object below waistline of worker
- Unsterile objects contacting sterile objects
- Sterile objects contacting the edge of a surgical tray.
- Healthcare worker leaning over the sterile field.
Infection Control Strategies
- Overview of infection control strategies
Routine Practices & Hand Hygiene
- Overview of routine practices & hand hygiene
Routine Practices History
- Evolution of precautions from Universal Precautions to Standard Precautions to Routine Practices & Additional Precautions.
Universal Precautions
- Avoid contact with patients' bodily fluids (1985-1988).
- Associated with HIV/AIDS epidemic
- 1987 saw addition of body substance precautions.
Standard Precautions
- Use of PPE recommended in all settings (1996)
Routine Practices & Additional Precautions
- Overview and implementation, as it relates to infection control (1999).
Additional Precautions
- Requirements to deal with difficult/dangerous pathogens (ex. Clostridium difficile or MRSA).
- Indicated with signage.
Routine Practices
- Point of Care Risk Assessment (PCRA)
- Environmental Controls
- Administrative Controls
PHAC (Public Health Agency of Canada)
- Created in 2004 in response to growing public health concerns.
- Created in response to recommendations from leading public health experts (e.g., Dr. David Naylor's report, Learning from SARS).
Additional Precautions
- Contact
- Droplet ( >5 microns, within 2 meters)
- Droplet/Contact
- Airborne ( <5 microns, greater than 2 meters)
At Risk Populations-Contracting Flu
- Individuals over 65
- LTC facilities/congregate living
- Those with pre-existing/chronic diseases
- Caregivers/those living with high risk individuals
At Risk Populations/Complications of Flu
- Over 65 (older adults)
- Chronic health conditions (adults & children)
- Pregnant women
- Indigenous populations
- Young Children
Is it Cold or Flu?
- Symptoms-cold vs. flu comparison table
Breaking the Chain of Infection
- An overview of the different aspects of breaking the chain of infection
Chain of Infection
- Infectious Agent
- Reservoir
- Portal of Exit
- Mode of Transmission
- Portal of Entry
- Susceptible Host
Infectious Agent: Antimicrobial Therapy: Disinfection; Sterilization
- Overview of various infection control strategies.
Reservoir: Engineering Controls: Environmental Cleaning; Disinfection; Proper Food Storage; Water Treatment
- Overview of strategies to prevent/control reservoirs for the spread of infection.
Portal of Exit: Hand Hygiene; Disposal of contaminated linens; Control of Excretions/Secretions
- Overview of methods and approaches to limit/manage portals of exit.
Means/Mode of Transmission: Spatial separation; Engineering Controls; Hand Hygiene; Environmental cleaning/disinfection; Device disinfection/sterilization; PPE
- Overview of strategies to reduce the methods of transmission including spatial separation/engineering controls/hand hygiene, environmental cleaning/disinfection, device disinfection/sterilization and PPE
Portal of Entry: Hand Hygiene; Aseptic Technique; Wound Care; Catheter Care; PPE
- Overview and examples of strategies for limiting portals of entry.
Susceptible Host: Immunization; Nutrition; Recognition of high risk patients; Treatments
- Overview and examples of strategies to limit/manage susceptible factors.
Antimicrobial Therapy
- An overview of antimicrobial therapy.
Rise in Life Expectancy
- The impact of sanitation, antibiotics, and vaccines on increased life expectancy.
Types of Vaccines
- Live-attenuated
- Inactivated
- Subunit
- Toxoid
- mRNA
- Viral Vector
Antibiotics
- Alexander Fleming, and Penicillin
Antibiotic Resistance
- When an organism becomes resistant to an antibiotic
- Strategies used by microorganisms to develop resistance: access restriction of antibiotics, antibiotic destruction via enzyme secretion, efflux pumps (removal of antibiotics from cell), metabolic pathways modification, overproduction of target molecules, structural changes/mutation.
- Mechanisms of resistance acquisition (random mutation, horizontal/lateral gene transfer).
Antibiotic Resistance: Increasing
- Resistance is developing faster than new antibiotics are being developed.
- Misuse of antibiotics increases selective pressure on bacteria, accelerating resistance development.
Summary: Methods to Control Pathogens
- Host defense mechanisms
- Public health measures
- Vaccines
- Antibiotics
Define Health Care Associated Infection (HAI)
- Hospital-acquired infections (HAIs) - infections resulting from hospital stays or other healthcare settings; usually not present or incubating at the time of admission.
Monitoring HAI's
- CNISP (Canadian Nosocomial Infection Surveillance Program), maintained by PHAC to track/monitor HAIs in healthcare settings.
Types of HAI's
- Antimicrobial-resistant organisms (AROs)
- Bloodstream infections (BSIs)
- Urinary tract infections (UTIs)
- Surgical site infections (SSIs)
- Pneumonia
- Infectious Diseases
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Description
This quiz serves as a final review for the Microbiology and Infection Control course at RRC Polytech. It covers essential topics such as the history of microbiology, classifications of microorganisms, and the body's natural defense mechanisms against infections.