Microbiology Anaerobic Techniques
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Questions and Answers

What primary function do anaerobic glove boxes and work stations serve in microbiology?

  • To provide an oxygen-rich environment for bacteria
  • To sterilize culture media before use
  • To process specimens without oxygen exposure (correct)
  • To speed up bacterial growth
  • Which of the following is a chemical indicator used to confirm the presence of anaerobic conditions?

  • Sodium bicarbonate
  • Sodium borohydride
  • Methylene blue (correct)
  • Palladium catalyst
  • What does the absence of growth in a culture of Pseudomonas indicate within an anaerobic culture system?

  • The presence of an obligate anaerobe
  • Complete anaerobiosis (correct)
  • Incomplete anaerobiosis
  • Complete oxygen presence
  • What is examined after overnight incubation of culture media to assess bacterial growth?

    <p>Colony morphology</p> Signup and view all the answers

    What type of biochemical test would most likely be performed initially on all colony types?

    <p>Catalase and oxidase tests</p> Signup and view all the answers

    Which enzyme's presence is detected by the oxidase test?

    <p>Cytochrome oxidase</p> Signup and view all the answers

    What characterizes β-hemolysis on a blood agar plate?

    <p>Complete clearing of blood around the colonies</p> Signup and view all the answers

    A deep purple color within 10 seconds during an oxidase test indicates what result?

    <p>A positive result, indicating presence of cytochrome oxidase</p> Signup and view all the answers

    What is the purpose of the catalase test?

    <p>To determine if a bacteria produces catalase</p> Signup and view all the answers

    Which of the following bacterial species is associated with α-hemolysis on blood agar plates?

    <p><em>Pneumococci</em></p> Signup and view all the answers

    Which gases are produced during the chemical process within the GasPak anaerobic system's envelope?

    <p>Hydrogen and carbon dioxide</p> Signup and view all the answers

    The appearance of bubbles when hydrogen peroxide is added to a bacterial colony indicates a ____ result for the catalase test.

    <p>Positive</p> Signup and view all the answers

    Which bacterial genera is primarily differentiated using the catalase test?

    <p>Staphylococcus and Streptococcus</p> Signup and view all the answers

    What enzyme activity is detected by the indole test?

    <p>Tryptophanase</p> Signup and view all the answers

    A cherry-red ring after adding Kovac's reagent to a culture indicates what result in the indole test?

    <p>A positive result, presence of indole</p> Signup and view all the answers

    Which of these bacteria is indole-negative?

    <p>Pseudomonas</p> Signup and view all the answers

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