Mendel's Experiments: Monohybrid Cross

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Questions and Answers

Consider a scenario where two genes, A and B, are located on the same chromosome but are far enough apart to allow for occasional recombination. If a plant with genotype AaBb is self-pollinated, what phenotypic ratio would you expect to observe in the progeny if complete linkage were to occur?

  • 3:1, reflecting the dominance of A and B alleles.
  • 1:1, indicating only parental phenotypes. (correct)
  • 9:3:3:1, representing independent assortment.
  • 1:2:1, suggesting incomplete dominance.

In a trihybrid cross involving three independently assorting genes, the expected proportion of offspring showing all three recessive traits is 1/64, assuming each gene exhibits complete dominance

True (A)

Define the concept of a testcross and explain its utility in determining the genotype of an organism displaying a dominant phenotype. Assume single-gene inheritance with complete dominance.

A testcross involves crossing an individual with a dominant phenotype but unknown genotype with a homozygous recessive individual. If any recessive offspring appear, the dominant individual must be heterozygous.

Mendel's law of ______ states that during gamete formation, allele pairs segregate randomly and each gamete receives only one allele of each gene.

<p>segregation</p> Signup and view all the answers

Match the following terms with their correct definitions in the context of Mendelian genetics:

<p>Genotype = The genetic makeup of an organism, typically referring to the specific alleles present. Phenotype = The observable physical or biochemical characteristics of an organism, resulting from the interaction of its genotype with the environment. Homozygous = Having two identical alleles for a particular gene. Heterozygous = Having two different alleles for a particular gene.</p> Signup and view all the answers

Consider a population of plants where flower color is determined by a single gene with two alleles: $R$ (red) and $r$ (white). Red is dominant to white. If the frequency of the $R$ allele is 0.7, what is the expected frequency of heterozygotes ($Rr$) in the population, assuming the population is in Hardy-Weinberg equilibrium?

<p>0.42 (D)</p> Signup and view all the answers

The law of independent assortment always holds true for genes located on the same chromosome, regardless of the distance between them.

<p>False (B)</p> Signup and view all the answers

Explain how epistasis can modify the expected phenotypic ratios in a dihybrid cross, providing a specific example and expected ratio.

<p>Epistasis occurs when one gene masks or modifies the expression of another gene. For example, in Labrador Retrievers coat color, the E gene (E/e) determines whether pigment is deposited (E) or not (e), regardless of the B gene (B/b) that determines black or brown color. An ee individual will be yellow, regardless of their B allele, resulting in a modified 9:3:4 ratio instead of the typical 9:3:3:1.</p> Signup and view all the answers

The phenomenon where a single gene affects multiple traits is called ______.

<p>pleiotropy</p> Signup and view all the answers

In a plant species, the height is controlled by three genes, each with two alleles (A/a, B/b, C/c) that have additive effects. The shortest plant is 30 cm, and the tallest is 60 cm. If a plant with genotype AABBCC is crossed with a plant with genotype aabbcc, what is the expected height of the F1 generation?

<p>45 cm (D)</p> Signup and view all the answers

Consider a scenario where Gregor Mendel, faced with limited resources and a sudden aphid infestation in his primary pea plant population, decides to perform a modified dihybrid cross using only F1 generation plants heterozygous for both seed color (Yy) and seed shape (Rr). Given that aphid feeding preferentially damages ovules carrying the dominant 'R' allele, leading to a 20% reduction in their viability. What would be the expected phenotypic ratio of the F2 generation?

<p>A ratio skewed towards recessive traits, such as 6:2:2:1, reflecting the decreased representation of the dominant 'R' allele due to aphid-induced ovule damage. (B)</p> Signup and view all the answers

Assume that Mendel had access to modern molecular techniques, and further discovered that the gene controlling plant height in his pea plants was actually located on a non-recombining region, otherwise known as a holocentric chromosome. Would Mendel's Law of Independent Assortment still hold true for plant height?

<p>False (B)</p> Signup and view all the answers

Imagine Mendel discovered a novel epigenetic modification that affected gene expression based on parental origin in pea plants, contrary to his findings. How would he explain the observed patterns of inheritance if the expression of a certain trait depended on whether it was inherited from the maternal or paternal line?

<p>Mendel would explore possible mechanisms of parent-specific gene silencing or modifications during gametogenesis that influence the expression of alleles, as such epigenetic phenomena defy Mendel's original postulates of equal allelic contribution.</p> Signup and view all the answers

In a hypothetical scenario where Mendel encountered a case of incomplete dominance in flower color, where a homozygous red-flowered plant crossed with a homozygous white-flowered plant produces all pink-flowered offspring, and the F2 generation shows a 1:2:1 ratio of red:pink:white flowers, this would be considered a violation fo the Law of ______.

<p>Dominance</p> Signup and view all the answers

Match the modern genetic concepts with their closest analogous element in Mendel's original experiments and postulates:

<p>Gene = Factor Allele = Trait variants Homozygous = True-breeding lines Segregation = Separation of factors during gamete formation</p> Signup and view all the answers

Suppose Mendel's pea plants had a gene that exhibited overdominance (heterozygote advantage) for disease resistance, where heterozygous individuals show higher resistance than either homozygous type. If a farmer consistently crosses two true-breeding lines, one highly susceptible (rr) and the other with low yield but moderate resistance (RR), what would be the long-term implications for the genetic diversity and overall yield of the farmer's pea crop?

<p>Stabilization of genetic diversity at a point where heterozygotes are maintained, but overall yield remains limited due to the constant presence of the low-yielding RR homozygotes. (D)</p> Signup and view all the answers

Assume Mendel repeated his monohybrid cross experiments with pea plants that are tetraploid (4n) instead of diploid (2n). The law of segregation would still hold true, with the only difference being a higher number of possible allelic combinations in the gametes.

<p>False (B)</p> Signup and view all the answers

Imagine Mendel discovered that certain crosses in pea plants consistently produced offspring with new, stable phenotypes not present in either parent. Formulate a hypothesis based on modern genetic mechanisms to explain how these novel phenotypes could arise and become stably inherited.

<p>The new, stable phenotypes could result from epigenetic modifications, such as alterations in DNA methylation or histone acetylation patterns, that are induced by the cross and then stably inherited across generations, leading to altered gene expression and novel traits.</p> Signup and view all the answers

If Mendel had studied a trait governed by a gene located on the mitochondrial DNA of pea plants, he would have observed a pattern of inheritance known as ______ inheritance, where the trait is exclusively passed down from the maternal parent.

<p>Maternal</p> Signup and view all the answers

Match each scenario with the most relevant Mendelian law or principle it violates or extends:

<p>A gene influencing multiple seemingly unrelated traits = Pleiotropy (extension) Alleles that blend their effects in heterozygotes = Incomplete Dominance (violation of Dominance) Two genes on the same chromosome that are almost always inherited together = Linkage (violation of Independent Assortment) The expression of a gene varies depending on whether it was inherited from the mother or father = Genomic Imprinting (violation of Independent Assortment)</p> Signup and view all the answers

Flashcards

Inheritance

The process where offspring receive genetic information from their parents, leading to similar characteristics within families.

Gregor Mendel

Scientist who formulated laws of inheritance by experimenting with pea plants in the mid-19th century.

True-breeding pea lines

Pea lines that consistently show the same traits after self-pollination.

Genes

Units of inheritance passed down in a stable manner from parents to offspring.

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Monohybrid cross

An experiment where Mendel crossed pea plants with opposite traits (e.g., tall and short).

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F1 cross

A cross of the first generation offspring (F1) from a monohybrid cross.

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Law of Segregation

The principle that during gamete formation, allele pairs separate so that each gamete receives only one allele.

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Law of Dominance

The principle that in a heterozygote, one allele (the dominant one) masks the effect of the other allele (the recessive one).

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Dihybrid cross

An experiment where Mendel considered two traits, each with two alleles.

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Phenotype

The observable physical characteristics of an organism.

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Genotype

The genetic makeup of an organism (the specific allele combination).

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Allele

Alternative forms of a gene that arise by mutation and are found at the same place on a chromosome.

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Homozygous Alleles

Having two identical alleles for a particular gene.

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Heterozygous Alleles

Having two different alleles for a particular gene.

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Dominant Trait

The allele that is expressed in the phenotype when present in a heterozygous condition.

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Recessive Trait

The allele that is masked in the phenotype by a dominant allele.

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Law of Independent Assortment

Genes for different traits assort independently of one another during gamete formation.

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Study Notes

  • Inheritance is how offspring receive genetic information from parents.
  • Heredity depends on inheritance, causing family members to share traits.
  • Understanding inheritance emerged in the mid-19th century.
  • Gregor Mendel developed laws of inheritance.

Mendel's Experiments

  • Mendel hybridized garden peas between 1856-1863.
  • He chose distinct pea characteristics, employing cross-pollination and artificial pollination. True-breeding pea lines showed stable inheritance.
  • Mendel selected pea plants because they grow easily, self-pollinate (but can cross-pollinate), are annual, and have contrasting traits.
  • Genes: factors transferred to offspring.
  • Mendel performed 2 main experiments to determine inheritance laws.

Monohybrid Cross

  • Mendel crossed pea plants with opposite traits (tall and short).
  • The first generation offspring (F1) were tall.
  • F1 cross produced tall and short plants in a 3:1 ratio.
  • Similar results occurred with green vs. yellow peas, round vs. wrinkled.
  • Mendel formulated the laws of Segregation and Dominance from these results.

Dihybrid Cross

  • Mendel considered two traits, each with two alleles.
  • He crossed wrinkled-green and round-yellow seeds and observed that F1 progeny were round-yellow (dominant traits).
  • Self-pollinating F1 produced wrinkled-yellow, round-yellow, wrinkled-green, and round-green traits in a 9:3:3:1 ratio.
  • Mendel formulated the Law of Independent Assortment from similar results with other traits.

Conclusions from Mendel's Experiments

  • Genotype: genetic makeup of a plant.
  • Phenotype: physical appearance of a plant.
  • Alleles: gene pairs transferred from parents to offspring.
  • Gametogenesis halves chromosomes, giving a 50% chance of either allele fusing with the other parent.
  • Homozygous alleles: alleles are the same.
  • Heterozygous alleles: alleles are different.

Mendel's Laws

  • Law of Dominance (Mendel's first law): hybrid offspring inherit the dominant phenotype trait.
  • Recessive traits are suppressed while alleles that determine the trait are known as dormant traits.
  • Law of Segregation: during gamete production, each hereditary factor's copies segregate so that offspring acquire one factor from each parent; allele pairs segregate during gamete formation and reunite during fertilization.
  • Law of Independent Assortment (Mendel's second law): trait pairs segregate independently during gamete formation, allowing traits equal opportunity to occur together.

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