Medicinal Plants Analysis of Northeast India
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Medicinal Plants Analysis of Northeast India

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Questions and Answers

Which process involves the preparation of plant extracts using hot water extraction?

  • Combining dried powder with acid
  • Mixing with iodine solution
  • Using a mechanical blender for grinding
  • Adding distilled water to powdered plant material (correct)
  • What indicated the presence of carbohydrate after adding H2SO4 in the test?

  • Production of gas bubbles
  • Appearance of a violet ring (correct)
  • Change in temperature of the solution
  • Formation of a yellow ring
  • Which of the following plants was collected from Sonitpur and Dibrugarh districts for medicinal use?

  • Ricinus communis (correct)
  • Aloe vera
  • Ginger
  • Lavender
  • What was the initial state of the plant materials before extraction?

    <p>Shade dried and moisture-free</p> Signup and view all the answers

    Which method was used for grinding the plant materials after drying?

    <p>Mechanical blender</p> Signup and view all the answers

    What is the purpose of transferring the ground plant material into airtight containers?

    <p>To prevent moisture and contamination</p> Signup and view all the answers

    Which of the following was specifically tested using an iodine solution?

    <p>Presence of carbohydrates</p> Signup and view all the answers

    What is the main step following the drying of plant materials for extraction?

    <p>Grinding the materials</p> Signup and view all the answers

    Which method was used to determine the total phenolic contents in the plant extracts?

    <p>Folins-Ciocalteus reagent method</p> Signup and view all the answers

    What type of plant parts were analyzed from Ricinus communis in the study?

    <p>Roots</p> Signup and view all the answers

    Which of the following plants had the highest total flavonoid content?

    <p>Terminalia bellerica</p> Signup and view all the answers

    What was the purpose of the study conducted on the seven medicinal plants?

    <p>To investigate the presence of phytochemicals</p> Signup and view all the answers

    Which solvent was NOT mentioned as being used for the organic solvent extraction?

    <p>Hexane</p> Signup and view all the answers

    What common secondary metabolites were detected in all of the plants tested?

    <p>Phenols, flavonoids, and tannins</p> Signup and view all the answers

    Which plant showed the lowest total phenolic content, according to the findings?

    <p>Bryophyllum pinnatum</p> Signup and view all the answers

    What does the study suggest about the crude aqueous and organic solvent extracts of the tested plants?

    <p>They contain multiple bioactive compounds.</p> Signup and view all the answers

    What does the appearance of a violet colour in the Ninhydrin test suggest?

    <p>Presence of amino acids and proteins</p> Signup and view all the answers

    Which test indicates the presence of reducing sugars when a brick red precipitate forms?

    <p>Fehling's test</p> Signup and view all the answers

    In the Keller-Kilani test, what indicates the presence of cardiac glycosides?

    <p>Brown ring at the interphase</p> Signup and view all the answers

    What is the main indication of a reddish brown colour forming in Salkowski’s test?

    <p>Presence of steroids</p> Signup and view all the answers

    Which reagent is involved in the Molisch's test for carbohydrate detection?

    <p>Molisch's reagent</p> Signup and view all the answers

    What signifies the presence of glycosides in Liebermann’s test?

    <p>Blue to green colour change</p> Signup and view all the answers

    Which of the following tests determines the presence of carbohydrates via a reddish brown precipitate?

    <p>Benedict's test</p> Signup and view all the answers

    What result would indicate the presence of saponins?

    <p>Stable foam formation</p> Signup and view all the answers

    What indicated the presence of carbohydrates in the water extract?

    <p>Blue or purple coloration</p> Signup and view all the answers

    Which solution was used to test for phenols and tannins?

    <p>2% FeCl3 solution</p> Signup and view all the answers

    What color change signifies the presence of flavonoids when using the Shinoda test?

    <p>Pink scarlet</p> Signup and view all the answers

    What is indicated by the formation of an intense yellow color with NaOH in the alkaline reagent test?

    <p>Presence of flavonoids</p> Signup and view all the answers

    What method was used for the crude extract preparation from powdered plant material?

    <p>Soxhlet extraction</p> Signup and view all the answers

    What is the outcome when the crude extract reacts with Millon’s reagent?

    <p>White precipitate formation</p> Signup and view all the answers

    In the test for saponins, what indicates a positive result?

    <p>Froth formation upon shaking</p> Signup and view all the answers

    How long was the crude extract heated continuously during the carbohydrate test?

    <p>20 minutes</p> Signup and view all the answers

    What was used as the standard for measuring the steroid test?

    <p>Gallic acid</p> Signup and view all the answers

    What indicates the presence of steroids in the chloroform test?

    <p>A red color in the lower chloroform layer</p> Signup and view all the answers

    Which method was utilized to determine the flavonoid content?

    <p>Aluminium chloride colorimetric method</p> Signup and view all the answers

    What indicates the presence of terpenoids in the chloroform test?

    <p>A grayish color</p> Signup and view all the answers

    How were the results for both steroids and terpenoids expressed?

    <p>As gallic acid equivalent (mg/g)</p> Signup and view all the answers

    What was the incubation period for the flavonoid content test?

    <p>30 minutes</p> Signup and view all the answers

    Which wavelength was used to measure the steroid test results?

    <p>765 nm</p> Signup and view all the answers

    What was included in the flavonoid content test besides the plant extract?

    <p>Methanol</p> Signup and view all the answers

    Study Notes

    Medicinal plants analysis

    • The study aimed to investigate the presence of phytochemicals and measure total phenolic and flavonoid content in seven medicinal plants from Northeast India.
    • Plant material selection included Bryophyllum pinnatum, Ipomea aquatica, Oldenlandia corymbosa, Ricinus communis, Terminalia bellerica, Tinospora cordifolia, and Xanthium strumarium.
    • The plants were dried, ground into powder, and extracted with water, methanol, ethanol, and acetone for further analysis.
    • The study used different methods to determine the presence of specific phytochemicals, including proteins, carbohydrates, phenols, tannins, flavonoids, saponins, glycosides, steroids, and terpenoids.

    Phytochemicals Identified

    • All seven plants exhibited a positive test for proteins, carbohydrates, phenols, tannins, flavonoids, and saponins.
    • The study did not specify which glycosides or steroids, just indicated their presence within each plant.
    • Each plant showed variability in its phytochemical concentration.

    Phenolic Content

    • The highest total phenolic content was found in the aqueous extract of Xanthium strumarium, followed by Tinospora cordifolia stem, Ricinus communis roots, and Terminalia bellerica leaves.
    • The lowest phenolic content was found in Oldenlandia corymbosa.

    Flavonoid Content

    • The highest total flavonoid content was found in the aqueous extract of Ipomea aquatica, followed by Terminalia bellerica, Tinospora cordifolia leaves, and Xanthium strumarium.
    • The lowest flavonoid content was found in Oldenlandia corymbosa and Tinospora cordifolia stem.

    Conclusion

    • The study concluded that the tested plants contain medically important bioactive compounds, supporting their traditional uses in medicine.
    • The findings highlight the potential of these plants for further research on pharmaceutical applications.

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    Description

    This quiz explores the analysis of phytochemicals in seven medicinal plants from Northeast India. It covers plant selection, extraction methods, and the identification of various phytochemicals such as flavonoids and phenols. Test your knowledge on the medicinal properties and chemical content of these plants.

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