12 Questions
What is the purpose of adding anticoagulant to blood before centrifugation?
To prevent clotting
What is the difference between venepuncture and cannulation?
Venepuncture collects discrete samples, while cannulation collects serial samples over several hours.
What is the purpose of allowing blood to clot at room temperature before centrifugation?
To collect serum
Where is Myosin ATPase found?
At cross bridges
What is the purpose of magnetic resonance spectroscopy?
To measure relative abundance in large muscle volumes
What happens when there is an increase in ATP concentration?
ATP binds to allosteric inhibitory sites on PFK, reducing the affinity to fructose 6-phosphate and slowing down glycolysis.
What is the purpose of allowing blood to clot at room temperature prior to centrifuge when collecting serum?
Prevent clotting and allow separation of serum from blood cells
What is the significance of measuring single fibre metabolite concentrations in muscle biopsies?
It provides specific insights into the metabolic activity of individual muscle fibers
What is the formula for calculating the phosphorylation potential?
(ATP x Pi) / ADP
Where is LDH-M primarily found?
Type II muscle fibers
What is the function of monocarboxylate transporters?
Responsible for the uptake and release of lactate
How does an increase in ATP concentration affect glycolysis through PFK inhibition?
Reduces the affinity to fructose 6-phosphate, slowing down glycolysis
Test your knowledge on medical sample collection methods such as venepuncture and cannulation, as well as different types of ATPase and enzyme reactions. This quiz covers topics related to collecting plasma and serum, as well as the reactions involving adenylate kinase and AMP deamination.
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