Medical Sample Collection and Enzyme Types Quiz

What is the purpose of adding anticoagulant to blood before centrifugation?

What is the difference between venepuncture and cannulation?

What is the purpose of allowing blood to clot at room temperature before centrifugation?

Where is Myosin ATPase found?

What is the purpose of magnetic resonance spectroscopy?

What happens when there is an increase in ATP concentration?

What is the purpose of allowing blood to clot at room temperature prior to centrifuge when collecting serum?

What is the significance of measuring single fibre metabolite concentrations in muscle biopsies?

What is the formula for calculating the phosphorylation potential?

Where is LDH-M primarily found?

What is the function of monocarboxylate transporters?

How does an increase in ATP concentration affect glycolysis through PFK inhibition?

Blood Collection and Processing

Anticoagulant is added to blood before centrifugation to prevent blood clotting and facilitate analysis of plasma or whole blood components.
Venepuncture involves a single puncture of a vein for blood collection, whereas cannulation involves the insertion of a cannula (a small tube) into a vein for continuous blood sampling or infusion.
Allowing blood to clot at room temperature before centrifugation helps to obtain serum, which is the liquid portion of blood that remains after clotting.

Enzymes and Metabolic Pathways

Myosin ATPase is an enzyme found in muscle cells that catalyzes the hydrolysis of ATP to generate mechanical energy for muscle contraction.

Spectroscopy and Metabolite Analysis

Magnetic resonance spectroscopy (MRS) is a non-invasive technique used to measure the concentration of certain metabolites, such as ATP, in tissues.
When ATP concentration increases, it inhibits phosphofructokinase (PFK), a key enzyme in glycolysis, thereby reducing glycolytic flux.

Muscle Biopsy and Metabolite Analysis

Measuring single fibre metabolite concentrations in muscle biopsies helps to understand the metabolic profiles of different fibre types and their responses to exercise or disease.
The phosphorylation potential can be calculated using the formula: phosphorylation potential = [ATP]/[ADP][Pi]

Enzymes and Transporters

LDH-M (lactate dehydrogenase M) is primarily found in muscles and catalyzes the interconversion of pyruvate and lactate.
Monocarboxylate transporters (MCTs) are proteins that facilitate the transport of monocarboxylates, such as lactate, across cell membranes.

Test your knowledge on medical sample collection methods such as venepuncture and cannulation, as well as different types of ATPase and enzyme reactions. This quiz covers topics related to collecting plasma and serum, as well as the reactions involving adenylate kinase and AMP deamination.