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Medical Microbiology: Sterilization & Disinfection

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Medical Microbiology: Sterilization & Disinfection

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Questions and Answers

What is the primary goal of sterilization in medical microbiology?

To kill vegetative bacteria only

To eliminate all pathogenic microorganisms

To remove or destroy all microorganisms including bacterial spores, viruses, and fungi (correct)

To prevent the growth of fungi

Which of the following is a characteristic of antiseptics?

They are non-toxic for superficial application on living tissue (correct)

They are effective against bacterial spores

They are toxic to human tissue

They are suitable for non-living objects only

What is the primary difference between disinfection and sterilization?

Sterilization is used for living tissue, while disinfection is used for non-living objects

Disinfection is used for living tissue, while sterilization is used for non-living objects

Disinfection eliminates all microorganisms, while sterilization does not

Sterilization eliminates all microorganisms, while disinfection does not (correct)

Which level of disinfectant is effective against vegetative bacteria, fungi, and viruses, but does not kill bacterial spores?

High-level disinfectant Signup and view all the answers

What is the most effective method of sterilization?

Heat Signup and view all the answers

Which of the following is NOT a method of sterilization?

Disinfection Signup and view all the answers

What is the mechanism of action of dry heat sterilization?

Destructive oxidation of the cell constituents Signup and view all the answers

What is the primary use of incineration in the medical microbiology laboratory?

Disposal of infectious hospital waste Signup and view all the answers

What is the temperature and time required for sterilization using a hot air oven?

160 ⁰C for 2 hours or 180 ⁰C for 1 hour Signup and view all the answers

What is the purpose of Tyndallization or fractional sterilization?

To sterilize heat-labile media Signup and view all the answers

What is the temperature used for pasteurization of milk using the holding method?

63 ⁰C for 30 minutes Signup and view all the answers

What is the purpose of red heat in sterilization?

To sterilize bacteriological loops Signup and view all the answers