3 Questions
Which of the following statements about restriction enzymes is true?
They recognize the same sequence in all organisms.
What is the advantage of using plasmids with only 1 to 10 sites of cutting in cloning?
They are more valuable
What can be done if common restriction sites are not available?
Alternative strategies can be employed
Study Notes
- Restriction enzymes cut DNA at specific sequences.
- Some restriction enzymes cut non-palindromic sequences.
- Fragments produced by the same enzyme on different organisms have the same cohesive ends.
- Most restriction enzymes recognize a unique sequence.
- The number of cuts made in DNA by a particular enzyme is limited.
- Bacterial chromosome DNA is cut into several-hundred-to-several-thousand fragments.
- Mammalian nuclear DNA is cut into more than a million fragments.
- Plasmids with only 1 to 10 sites of cutting are valuable in cloning.
- Cleaved DNAs can be ligated using cohesive, compatible ends.
- Alternative strategies can be employed if common restriction sites are not available.
Test your knowledge of restriction enzymes and their role in molecular biology with this quiz. Learn about the unique properties of these enzymes, including their ability to recognize specific DNA sequences and cut them into fragments with cohesive ends. Discover how these fragments are used in cloning and other molecular techniques. Explore alternative strategies for DNA manipulation if common restriction sites are not available. Keywords: restriction enzymes, DNA sequences, cohesive ends, cloning, molecular techniques.
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