Mastering Daily Controls and Sample Effects Testing

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Which step should be taken immediately upon receiving customer products?

Perform sample effects testing

Label all products with logical codes

Perform the daily control protocol

Inspect the customer products for damage (correct)

What is the purpose of the daily control?

To check the pH, sterility, and RLU levels of the customer product sample

To flag any likely issues with the customer products

To ensure no damage occurred during shipping

To assess the product's suitability for testing with the Innovate system (correct)

How many product packs are required for background and baseline checks?

One

Two (correct)

Three

Four

What should be done with the needle and syringe used for aliquoting?

Dispose of them in a sharps bin Signup and view all the answers

What do background readings measure?

The total ATP in the product sample Signup and view all the answers

What is the purpose of streaking out organisms prior to starting the bacterial challenge study?

To create the overnight culture Signup and view all the answers

What is the recommended concentration of microorganisms to spike into each product?

10 CFU/ml Signup and view all the answers

After incubating the spiked products for 24 hours, what should be done next?

Spray into a decontaminated biosafety cabinet Signup and view all the answers

At which temperature should TSA plates be incubated?

37°C Signup and view all the answers

Where can the Innovate results template be found?

Guildford R&D > Innovate folder on OneDrive Signup and view all the answers

Which of the following should be done before starting any work?

Contact the customer about broken samples Signup and view all the answers

What should be done if any consumables need to be ordered?

Order them in advance to avoid delays Signup and view all the answers

Which reagents should be checked for their expiration dates?

Innovate reagents Signup and view all the answers

What should be done if any samples are broken or have leaked?

Contact the customer and request more samples Signup and view all the answers

When should the equipment necessary for the project be labeled?

At least 1 day in advance of starting the project Signup and view all the answers

Which of the following is NOT a step in preparing the Biosafety Cabinet for Innovate work?

Aliquoting the sample from the packaging Signup and view all the answers

When should the ATX and Dilutor DA bottles be opened?

Before pouring the contents of Dilutor DA to the ATX bottle Signup and view all the answers

What should be done if there is not enough Cellsolver DS in the bottle for the run?

Take out a new bottle from the deli fridge Signup and view all the answers

What should be done after pouring the contents of Dilutor DA to the ATX bottle?

Leave to resuspend for 2 minutes Signup and view all the answers

When should the daily control be run on each Innovate?

Before any work is done on the machine Signup and view all the answers

Which of the following should be done to ensure that all consumables are available before starting the project?

Order more Innovate or consumables in advance Signup and view all the answers

What should be done if any samples are broken or have leaked?

Contact the customer and request more samples Signup and view all the answers

When should the equipment necessary for the project be labeled?

At least 1 day in advance of starting the project Signup and view all the answers

What should be done if there is not enough Innovate or any Hygiena’s consumable for the project?

Contact <a href="mailto:[email protected]">[email protected]</a> to order more Signup and view all the answers

What should be done with the needle and syringe used for aliquoting?

Follow proper disposal procedures Signup and view all the answers

Which of the following is NOT a step in preparing the Biosafety Cabinet for Innovate work?

Reconstituting reagents on the bench Signup and view all the answers

What is the purpose of streaking out organisms prior to starting the bacterial challenge study?

To separate and isolate individual bacterial colonies Signup and view all the answers

What should be done after pouring the contents of Dilutor DA to the ATX bottle?

Leave it to resuspend for 2 minutes Signup and view all the answers

Which reagents should be checked for their expiration dates?

Sensilux and Cellsolver DS Signup and view all the answers

What do background readings measure?

The baseline readings of the empty wells of the plate Signup and view all the answers

Which step should be taken immediately upon receiving customer products?

Inspect the products for damage Signup and view all the answers

What is the purpose of the background readings in sample effects testing?

To measure the total ATP in the product sample Signup and view all the answers

What should be done with the needle and syringe used for aliquoting?

Dispose of them in a sharps bin Signup and view all the answers

When should the pH meter be calibrated?

Before each incubation period Signup and view all the answers

What is the purpose of the serial halving dilutions in sample effects measurements?

To create a range of dilutions for testing Signup and view all the answers

Which of the following is the correct order of steps for preparing the shoe goo seal on the product container?

Label the container, apply shoe goo seal, spray with Klericide Signup and view all the answers

What is the purpose of the dilution series of microorganisms in ultrapure water?

To obtain 100 CFU/ml concentration of each microorganism Signup and view all the answers

What is the recommended incubation temperature for SabDex plates?

30°C Signup and view all the answers

Where should the Innovate results template be downloaded from?

The Guildford R&D > Innovate folder on OneDrive Signup and view all the answers

What should be done with the spread plates after incubating them for 24 hours?

Record the results on the spreadsheet Signup and view all the answers

Mastering Daily Controls and Sample Effects Testing

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Questions and Answers

Which step should be taken immediately upon receiving customer products?

What is the purpose of the daily control?

How many product packs are required for background and baseline checks?

What should be done with the needle and syringe used for aliquoting?

What do background readings measure?

What is the purpose of streaking out organisms prior to starting the bacterial challenge study?

What is the recommended concentration of microorganisms to spike into each product?

After incubating the spiked products for 24 hours, what should be done next?

At which temperature should TSA plates be incubated?

Where can the Innovate results template be found?

Which of the following should be done before starting any work?

What should be done if any consumables need to be ordered?

Which reagents should be checked for their expiration dates?

What should be done if any samples are broken or have leaked?

When should the equipment necessary for the project be labeled?

Which of the following is NOT a step in preparing the Biosafety Cabinet for Innovate work?

When should the ATX and Dilutor DA bottles be opened?

What should be done if there is not enough Cellsolver DS in the bottle for the run?

What should be done after pouring the contents of Dilutor DA to the ATX bottle?

When should the daily control be run on each Innovate?

Which of the following should be done to ensure that all consumables are available before starting the project?

What should be done if any samples are broken or have leaked?

When should the equipment necessary for the project be labeled?

What should be done if there is not enough Innovate or any Hygiena’s consumable for the project?

What should be done with the needle and syringe used for aliquoting?

Which of the following is NOT a step in preparing the Biosafety Cabinet for Innovate work?

What is the purpose of streaking out organisms prior to starting the bacterial challenge study?

What should be done after pouring the contents of Dilutor DA to the ATX bottle?

Which reagents should be checked for their expiration dates?

What do background readings measure?

Which step should be taken immediately upon receiving customer products?

What is the purpose of the background readings in sample effects testing?

What should be done with the needle and syringe used for aliquoting?

When should the pH meter be calibrated?

What is the purpose of the serial halving dilutions in sample effects measurements?

Which of the following is the correct order of steps for preparing the shoe goo seal on the product container?

What is the purpose of the dilution series of microorganisms in ultrapure water?

What is the recommended incubation temperature for SabDex plates?

Where should the Innovate results template be downloaded from?

What should be done with the spread plates after incubating them for 24 hours?

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