Mastering Daily Controls and Sample Effects Testing

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AmiablePrudence
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40 Questions

Which step should be taken immediately upon receiving customer products?

Inspect the customer products for damage

What is the purpose of the daily control?

To assess the product's suitability for testing with the Innovate system

How many product packs are required for background and baseline checks?

Two

What should be done with the needle and syringe used for aliquoting?

Dispose of them in a sharps bin

What do background readings measure?

The total ATP in the product sample

What is the purpose of streaking out organisms prior to starting the bacterial challenge study?

To create the overnight culture

What is the recommended concentration of microorganisms to spike into each product?

10 CFU/ml

After incubating the spiked products for 24 hours, what should be done next?

Spray into a decontaminated biosafety cabinet

At which temperature should TSA plates be incubated?

37°C

Where can the Innovate results template be found?

Guildford R&D > Innovate folder on OneDrive

Which of the following should be done before starting any work?

Contact the customer about broken samples

What should be done if any consumables need to be ordered?

Order them in advance to avoid delays

Which reagents should be checked for their expiration dates?

Innovate reagents

What should be done if any samples are broken or have leaked?

Contact the customer and request more samples

When should the equipment necessary for the project be labeled?

At least 1 day in advance of starting the project

Which of the following is NOT a step in preparing the Biosafety Cabinet for Innovate work?

Aliquoting the sample from the packaging

When should the ATX and Dilutor DA bottles be opened?

Before pouring the contents of Dilutor DA to the ATX bottle

What should be done if there is not enough Cellsolver DS in the bottle for the run?

Take out a new bottle from the deli fridge

What should be done after pouring the contents of Dilutor DA to the ATX bottle?

Leave to resuspend for 2 minutes

When should the daily control be run on each Innovate?

Before any work is done on the machine

Which of the following should be done to ensure that all consumables are available before starting the project?

Order more Innovate or consumables in advance

What should be done if any samples are broken or have leaked?

Contact the customer and request more samples

When should the equipment necessary for the project be labeled?

At least 1 day in advance of starting the project

What should be done if there is not enough Innovate or any Hygiena’s consumable for the project?

Contact [email protected] to order more

What should be done with the needle and syringe used for aliquoting?

Follow proper disposal procedures

Which of the following is NOT a step in preparing the Biosafety Cabinet for Innovate work?

Reconstituting reagents on the bench

What is the purpose of streaking out organisms prior to starting the bacterial challenge study?

To separate and isolate individual bacterial colonies

What should be done after pouring the contents of Dilutor DA to the ATX bottle?

Leave it to resuspend for 2 minutes

Which reagents should be checked for their expiration dates?

Sensilux and Cellsolver DS

What do background readings measure?

The baseline readings of the empty wells of the plate

Which step should be taken immediately upon receiving customer products?

Inspect the products for damage

What is the purpose of the background readings in sample effects testing?

To measure the total ATP in the product sample

What should be done with the needle and syringe used for aliquoting?

Dispose of them in a sharps bin

When should the pH meter be calibrated?

Before each incubation period

What is the purpose of the serial halving dilutions in sample effects measurements?

To create a range of dilutions for testing

Which of the following is the correct order of steps for preparing the shoe goo seal on the product container?

Label the container, apply shoe goo seal, spray with Klericide

What is the purpose of the dilution series of microorganisms in ultrapure water?

To obtain 100 CFU/ml concentration of each microorganism

What is the recommended incubation temperature for SabDex plates?

30°C

Where should the Innovate results template be downloaded from?

The Guildford R&D > Innovate folder on OneDrive

What should be done with the spread plates after incubating them for 24 hours?

Record the results on the spreadsheet

Test your knowledge on running daily controls and sample effects testing with this quiz. Learn the proper steps to follow, from running the plate to storing it for future use. Challenge yourself with questions on plate preparation and sample effects.

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