Marker Gene Amplicon Data Analysis

Marker gene approaches are sensitive to technical factors such as primer choice.

The first step in analyzing marker gene amplicon data is to remove sequencing errors.

The rate of sequencing error in Illumina sequencing is approximately 0.1% per nucleotide.

<p>OTU picking clusters similar sequences into OTUs, consolidating similar sequences into single features.</p> Signup and view all the answers

<p>The OTU picking method misses subtle and real biological sequence variation, such as SNPs.</p> Signup and view all the answers