Marker Gene Amplicon Data Analysis
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Questions and Answers

What are marker gene approaches sensitive to?

Marker gene approaches are sensitive to technical factors such as primer choice.

What is the first step in analyzing marker gene amplicon data?

The first step in analyzing marker gene amplicon data is to remove sequencing errors.

What is the rate of sequencing error in Illumina sequencing?

The rate of sequencing error in Illumina sequencing is approximately 0.1% per nucleotide.

What does OTU picking do?

<p>OTU picking clusters similar sequences into OTUs, consolidating similar sequences into single features.</p> Signup and view all the answers

What does OTU picking method miss?

<p>The OTU picking method misses subtle and real biological sequence variation, such as SNPs.</p> Signup and view all the answers

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