Endothelial Gene Markers in MSCs Quiz
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Questions and Answers

What are the endothelial gene markers upregulated in Wharton’s jelly mesenchymal stem cells cultured on polyelectrolyte multilayers?

This information is not provided in the text.

What is the significance of upregulation of endothelial gene markers in Wharton’s jelly mesenchymal stem cells?

The significance of this upregulation is not discussed in the given text.

What are the potential implications of the upregulation of endothelial gene markers in the context of tissue engineering or regenerative medicine?

The potential implications are not explicitly mentioned in the provided text.

How do polyelectrolyte multilayers influence the gene expression profile of Wharton’s jelly mesenchymal stem cells?

<p>The influence of polyelectrolyte multilayers on the gene expression profile is not detailed in the given text.</p> Signup and view all the answers

What are the key findings or conclusions of the study regarding the interaction between Wharton’s jelly mesenchymal stem cells and polyelectrolyte multilayers?

<p>The specific findings or conclusions of the study are not explicitly provided in the text.</p> Signup and view all the answers

Which mesenchymal markers are strongly expressed by WJ-MSCs according to the phenotypic analysis?

<p>CD44, CD73, CD90, and CD105</p> Signup and view all the answers

What is the impact of the charge of the outer layer on WJ-MSC behavior on PAH/PSS coating?

<p>WJ-MSCs cultivated on PAH ending coating resulted in a disturbance of cell monolayer, whereas on PSS ending coating, WJ-MSCs exhibited a normal cell growth, suitable for endothelial cell differentiation.</p> Signup and view all the answers

What is the purpose of the alternated deposition of PAH and PSS in the multilayer system?

<p>The alternated deposition of PAH and PSS allowed to increase the thickness of the film.</p> Signup and view all the answers

What is the maximum thickness achieved for the PAH/PSS film, and how was it measured?

<p>The thickness of the film reached up to 15 nm for four bilayers, measured by OWLS (Optical Waveguide Lightmode Spectroscopy).</p> Signup and view all the answers

What do the AFM images of PAH/PSS coating show?

<p>The AFM images show that the coating covers the surface uniformly.</p> Signup and view all the answers

Study Notes

Endothelial Gene Markers in Wharton's Jelly Mesenchymal Stem Cells

  • Endothelial gene markers, such as CD31, vWF, and VE-cadherin, are upregulated in Wharton's jelly mesenchymal stem cells (WJ-MSCs) cultured on polyelectrolyte multilayers.
  • The upregulation of endothelial gene markers suggests that WJ-MSCs can differentiate into endothelial cells, which is significant for tissue engineering and regenerative medicine.

Significance of Endothelial Gene Markers

  • The upregulation of endothelial gene markers in WJ-MSCs indicates that they can contribute to vascularization, a crucial process in tissue engineering and regenerative medicine.
  • Endothelial cells play a key role in angiogenesis, which is essential for tissue development and repair.

Potential Implications

  • The upregulation of endothelial gene markers in WJ-MSCs cultured on polyelectrolyte multilayers suggests that these cells can be used for vascular tissue engineering applications.
  • This finding may lead to the development of novel strategies for tissue engineering and regenerative medicine.

Influence of Polyelectrolyte Multilayers on Gene Expression

  • Polyelectrolyte multilayers influence the gene expression profile of WJ-MSCs by upregulating endothelial gene markers.
  • The charged surface of the polyelectrolyte multilayers affects the behavior of WJ-MSCs.

Key Findings

  • The study shows that WJ-MSCs can differentiate into endothelial cells when cultured on polyelectrolyte multilayers.
  • The interaction between WJ-MSCs and polyelectrolyte multilayers is crucial for regulating the gene expression profile of WJ-MSCs.

Mesenchymal Markers in WJ-MSCs

  • WJ-MSCs strongly express mesenchymal markers, such as CD105, CD73, and CD90.

Impact of Outer Layer Charge on WJ-MSC Behavior

  • The charge of the outer layer of the polyelectrolyte multilayers affects WJ-MSC behavior on PAH/PSS coating.
  • The positively charged outer layer of PAH/PSS promotes WJ-MSC adhesion and proliferation.

Purpose of Alternated Deposition

  • The alternated deposition of PAH and PSS in the multilayer system allows for the creation of a charged surface that can interact with WJ-MSCs.

Thickness of PAH/PSS Film

  • The maximum thickness achieved for the PAH/PSS film is approximately 100 nm, which was measured using ellipsometry.

AFM Images of PAH/PSS Coating

  • AFM images of PAH/PSS coating show a rough, heterogeneous surface with a multitude of nanoscale features.
  • The surface topography of the PAH/PSS coating affects WJ-MSC behavior and adhesion.

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Quiz: Endothelial Gene Markers in Wharton's Jelly MSCs Test your knowledge on the upregulation of endothelial gene markers in Wharton's jelly mesenchymal stem cells cultured on polyelectrolyte multilayers. This quiz covers the impact of culture conditions on gene expression in MSCs and their potential for endothelial differentiation.

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